Cytokine profiling of human peripheral blood CD4+ T lymphocytes reveals a new Th-subpopulation (Th6) characterized by IL-6

European Cytokine Network

Volumn 21, Issue 2, 2010, Pages 105-115

  Azizi Semrad, Ursula ^a   Krenbek, Dagmar ^b   Hofbauer, Günther ^a   Karanikas, Georgios ^a   Maldonado Gonzalez, Eduardo ^a   Pietschmann, Peter ^a   Willheim, Martin ^a  

^a MEDICAL UNIVERSITY OF VIENNA   (Austria)

^b Institute of Pathology and Bacteriology   (Austria)

Author keywords

Flow cytometry; GATA 3; IL 2; IL 6; Intracellular cytokine detection; TNF

Indexed keywords

CYTOKINE; GAMMA INTERFERON; INTERLEUKIN 17; INTERLEUKIN 4; INTERLEUKIN 6; TRANSCRIPTION FACTOR;

ARTICLE; CD4+ T LYMPHOCYTE; CELL DIVISION; CORRELATION ANALYSIS; HUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; T LYMPHOCYTE SUBPOPULATION;

CD4-POSITIVE T-LYMPHOCYTES; CYTOKINES; FLOW CYTOMETRY; HUMANS; INTERLEUKIN-6;

EID: 77953876743     PISSN: 11485493     EISSN: None     Source Type: Journal    
DOI: 10.1684/ecn.2010.0190     Document Type: Article

References (25)

1. Mosmann T, Cherwinski H, Bond M, Giedlin M, Coffman R. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol 1986; 136: 2348.
2. Romagnani S. Regulation of the T cell response. Clin Exp Allergy 2006; 36: 1357.
3. Liew FY. T(H)1 and T(H)2 cells: a historical perspective. Nat Rev Immunol 2002; 2: 55.
4. Gor D, Rose N, Greenspan N. TH1-TH2: a procrustean paradigm. Nat Immunol 2003; 4: 503.
5. Messi M, Giacchetto I, Nagata K, Lanzavecchia A, Natoli G, Sallusto F. Memory and flexibility of cytokine gene expression as separable properties of human T(H)1 and T(H)2 lymphocytes. Nat Immunol 2003; 4: 78. (Pubitemid 36104711)
6. Harrington L, Mangan P, Weaver C. Expanding the effector CD4 T-cell repertoire: the Th17 lineage. Curr Opin Immunol 2006; 18: 349.
7. Zhu J, Paul WE. CD4 T cells: fates, functions, and faults. Blood 2008; 112: 1557.
8. Willheim M, Thien R, Schrattbauer K, et al. Regulatory effects of 1alpha,25-dihydroxyvitamin D3 on the cytokine production of human peripheral blood lymphocytes. J Clin Endocrinol Metab 1999;84:3 739.
9. Pichler J, Gerstmayr M, Szépfalusi Z, Urbanek R, Peterlik M, Willheim M. 1 alpha,25(OH)2D3 inhibits not only Th1 but also Th2 differentiation in human cord blood T cells. Pediatr Res 2002; 52: 12. (Pubitemid 34665944)
10. Thien R, Baier K, Pietschmann P, Peterlik M, Willheim M. Interactions of 1 alpha,25-dihydroxyvitamin D3 with IL-12 and IL-4 on cytokine expression of human T lymphocytes. J Allergy Clin Immunol 2005; 116: 683. (Pubitemid 41267147)
11. Correale J, Ysrraelit MC, Gaitán MI. Immunomodulatory effects of Vitamin D in multiple sclerosis. Brain 2009; 132: 1146.
12. Smolders J, Thewissen M, Peelen E, et al. Vitamin D status is positively correlated with regulatory T cell function in patients with multiple sclerosis. PLoS One 2009; 4: e6635.
13. Ghoreishi M, Bach P, Obst J, Komba M, Fleet JC, Dutz JP. Expansion of antigen-specific regulatory T cells with the topical vitamin D analog calcipotriol. J Immunol 2009; 182: 6071.
14. Male D, Brostoff J, Roth DB, Roitt I. Immunology. Amsterdam: Mosby Elsevier, 2006: 220.
15. Huse M, Lillemeier B, Kuhns M, Chen D, Davis M. T cells use two directionally distinct pathways for cytokine secretion. Nat Immunol 2006; 7: 247.
16. Rostaing L, Tkaczuk J, Durand M, et al. Kinetics of intracytoplasmic Th1 and Th2 cytokine production assessed by flow cytometry following in vitro activation of peripheral blood mononuclear cells. Cytometry 1999; 35: 318.
17. Nitsche A, Zhang M, Clauss T, Siegert W, Brune K, Pahl A. Cytokine profiles of cord and adult blood leukocytes: differences in expression are due to differences in expression and activation of transcription factors. BMC Immunol 2007; 8: 18.
18. Hoyer KK, Dooms H, Barron L, Abbas AK. Interleukin-2 in the development and control of inflammatory disease. Immunol Rev 2008; 226: 19.
19. Pillai V, Karandikar NJ. Human regulatory T cells: a unique, stable thymic subset or a reversible peripheral state of differentiation? Immunol Lett 2007; 114: 9.
20. Langrish C, Chen Y, Blumenschein W, et al. IL-23 drives a pathogenic T cell population that induces autoimmune inflammation. J Exp Med 2005; 201: 233.
21. Nakae S, Iwakura Y, Suto H, Galli S. Phenotypic differences between Th1 and Th17 cells and negative regulation of Th1 cell differentiation by IL-17. J Leukoc Biol 2007; 81: 1258.
22. Andersson U, Matsuda T. Human interleukin 6 and tumor necrosis factor alpha production studied at a single-cell level. Eur J Immunol 1989; 19: 1157-60.
23. Kishimoto T. Interleukin-6: discovery of a pleiotropic cytokine. Arthritis Res Ther 2006; 8 (Suppl. 2): S2.
24. Workman CJ, Szymczak-Workman AL, Collison LW, Pillai MR, Vignali DA. The development and function of regulatory T cells. Cell Mol Life Sci 2009; 66: 2603.
25. Annunziato F, Cosmi L, Santarlasci V, et al. Phenotypic and functional features of human Th17 cells. J Exp Med 2007; 204: 1849.