Single cell RT-PCR analysis of tyrosine kinase receptor expression in adult rat retinal ganglion cells isolated by retinal sandwiching

Brain Research Protocols

Volumn 10, Issue 2, 2002, Pages 75-83

  Lindqvist, Niclas ^a   Vidal Sanz, Manuel ^b   Hallböök, Finn ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b UNIVERSITY OF MURCIA   (Spain)

Author keywords

Fluorogold (FG); Polymerase chain reaction (PCR); Retinal ganglion cell (rgc); Reverse transcription (RT)

Indexed keywords

BETA ACTIN; BRAIN DERIVED NEUROTROPHIC FACTOR RECEPTOR; EPIDERMAL GROWTH FACTOR RECEPTOR 2; FLUOROGOLD; NEUROTROPHIN 3 RECEPTOR; PROTEIN DERIVATIVE; PROTEIN RET; SCATTER FACTOR RECEPTOR; TYROSINE KINASE RECEPTOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL ISOLATION; CELL LABELING; CONTROLLED STUDY; CYTOLOGY; DISSECTION; DISSOCIATION; FEMALE; FLUORESCENCE; GENE EXPRESSION; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN EXPRESSION; RAT; RETINA; RETINA GANGLION CELL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEPARATION TECHNIQUE; SUPERIOR COLLICULUS;

ANIMALS; CELL SEPARATION; DISSECTION; DNA, COMPLEMENTARY; FEMALE; FLUORESCENT DYES; GENE EXPRESSION; MICROSCOPY, ULTRAVIOLET; ORGAN CULTURE TECHNIQUES; RATS; RATS, SPRAGUE-DAWLEY; RECEPTOR PROTEIN-TYROSINE KINASES; REPRODUCIBILITY OF RESULTS; RETINA; RETINAL GANGLION CELLS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; STILBAMIDINES; SUPERIOR COLLICULUS; VISUAL PATHWAYS;

EID: 0036808209     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(02)00184-8     Document Type: Article

References (20)

1. Bermingham-McDonogh O., McCabe K.L., Reh T.A. Effects of GGF/neuregulins on neuronal survival and neurite outgrowth correlate with erbB2/neu expression in developing rat retina. Development. 122:1996;1427-1438.
2. Cauli B., Audinat E., Lambolez B., Angulo M., Ropert N., Tsuzuki K., Hestrin S., Rossier J. Molecular and physiological diversity of cortical nonpyramidal cells. J. Neurosci. 17:1997;3894-3906.
3. Grigorenko E., Yeh H. Expression profiling of GABAA receptor beta-subunits in the rat retina. Vis. Neurosci. 11:1994;379-387.
4. Henry J., Olander K. Comparison of the effect of four viscoelastic agents on early postoperative intraocular pressure. J. Cataract Refract. Surg. 22:1996;960-966.
5. Hong Y., Thanos S. A quantitative approach to identify and isolate pure populations of fluorescently labeled adult retinal ganglion cells using a pressure-driven microaspiration technique. Neurosci. Lett. 214:1996;111-114.
6. Klöcker N., Bräunling F., Isenmann S., Bähr M. In vivo neurotrophic effects of GDNF on axotomized retinal ganglion cells. Neuroreport. 8:1997;3439-3442.
7. Koeberle P.D., Ball A.K. Effects of GDNF on retinal ganglion cell survival following axotomy. Vis. Res. 38:1998;1505-1515.
8. Maniatis T., Fritsch E., Sambrook J. Molecular Cloning: A Laboratory Manual. 1982;Cold Spring Harbor Publications, New York.
9. Ruano D., Lambolez B., Rossier J., Paternain A., Lerma J. Kainate receptor subunits expressed in single cultured hippocampal neurons: molecular and functional variants by RNA editing. Neuron. 14:1995;1009-1017.
10. Saarma M. GDNF - a stranger in the TGF-beta superfamily? Eur. J. Biochem. 267:2000;6968-6971.
11. Shiosaka S., Kiyama H., Tohyama M. A simple method for the separation of retinal sublayers from the entire retina with special reference to application for cell culture. J. Neurosci. Methods. 10:1984;229-235.
12. Simon P., Thanos S. Combined methods of retrograde staining, layer-separation and viscoelastic cell stabilization to isolate retinal ganglion cells in adult rats. J. Neurosci. Methods. 83:1998;113-124.
13. Sun W., Funakoshi H., Nakamura T. Differential expression of hepatocyte growth factor and its receptor, c-Met in the rat retina during development. Brain Res. 851:1999;46-53.
14. Suzuki A., Nomura S., Morii E., Fukuda Y., Kosaka J. Localization of mRNAs for trkB isoforms and p75 in rat retinal ganglion cells. J. Neurosci. Res. 54:1998;27-37.
15. Valenzuela D.M., Maisonpierre P.C., Glass D.J., Rojas E., Nunez L., Kong Y., Gies D.R., Stitt T.N., Ip N.Y., Yancopoulos G.D. Alternative forms of rat TrkC with different functional capabilities. Neuron. 10:1993;963-974.
16. Vidal-Sanz M., Villegas-Perez M., Bray G., Aguayo A. Persistent retrograde labeling of adult rat retinal ganglion cells with the carbocyanine dye diI. Exp. Neurol. 102:1988;92-101.
17. Villegas-Perez M., Vidal-Sanz M., Rasminsky M., Bray G., Aguayo A. Rapid and protracted phases of retinal ganglion cell loss follow axotomy in the optic nerve of adult rats. J. Neurobiol. 24:1993;23-36.
18. Yan Q., Wang J., Matheson C., Urich J. Glial cell line-derived neurotrophic factor (GDNF) promotes the survival of axotomized retinal ganglion cells in adult rats comparison to and combination with brain-derived neurotrophic factor (BDNF). J. Neurobiol. 38:1999;382-390.
19. Yang J., Tezel G., Patil R.V., Wax M.B. Flow cytometry for quantification of retrogradely labeled retinal ganglion cells by Fluoro-Gold. Curr. Eye Res. 21:2000;981-985.
20. Zangenberg G., Saiki R., Reynolds R. Multiplex PCR: optimisation guidelines. Innis M., Gelfand D., Sninsky J., PCR Applications; Protocols for Functional Genomics. 1999;73-94 Academic Press, San Diego, CA.