TR-FRET biochemical assays for detecting posttranslational modifications of p53

Journal of Biomolecular Screening

Volumn 15, Issue 5, 2010, Pages 569-575

  Dudek, Jeanne M ^a   Horton, Robert A ^a  

^a INVITROGEN CORPORATION   (United States)

Author keywords

Acetylation; P53; Phosphorylation; Ubiquitination

Indexed keywords

PROTEIN P53;

ACETYLATION; ARTICLE; DEACETYLATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; TIME RESOLVED FLUORESCENCE RESONANCE ENERGY TRANSFER; UBIQUITINATION;

ACETYLATION; BIOLOGICAL ASSAY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; PHOSPHORYLATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT FUSION PROTEINS; SIRTUINS; TUMOR SUPPRESSOR PROTEIN P53; UBIQUITINATION;

EID: 77953638708     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057110365898     Document Type: Article

References (13)

1. Kruse JP, Gu W.: Modes of p53 regulation. Cell 2009 ; 137: 609-622.
2. Toledo F., Wahl GM: Regulating the p53 pathway: in vitro hypotheses, in vivo veritas. Nat Rev Cancer 2006 ; 6: 909-923.
3. Lane DP, Hupp TR: Drug discovery and p53. Drug Discov Today 2003 ; 8: 347-355.
4. Brooks CL, Gu W.: Ubiquitination, phosphorylation and acetylation: the molecular basis for p53 regulation. Curr Opin Cell Biol 2003 ; 15: 164-171.
5. Lavin MF, Gueven N.: The complexity of p53 stabilization and activation. Cell Death Differ 2006 ; 13: 941-950.
6. Olsson A., Manzl C., Strasser A., Villunger A.: How important are posttranslational modifications in p53 for selectivity in target-gene transcription and tumour suppression ? Cell Death Differ 2007 ; 14: 1561-1575.
7. Borra MT, Smith BC, Denu JM: Mechanism of human SIRT1 activation by resveratrol. J Biol Chem 2005 ; 280: 17187-17195.
8. Dumaz N., Milne DM, Meek DW: Protein kinase CK1 is a p53-threonine 18 kinase which requires prior phosphorylation of serine 15. FEBS Lett 1999 ; 463: 312-316.
9. Carlson CB, Robers MB, Vogel KW, Machleidt T.: Development of LanthaScreen cellular assays for key components within the PI3K/AKT/ mTOR pathway. J Biomol Screen 2009 ; 14: 121-132.
10. Riddle SM, Vedvik KL, Hanson GT, Vogel KW: Time-resolved fluorescence resonance energy transfer kinase assays using physiological protein substrates: applications of terbium-fluorescein and terbium-green fluorescent protein fluorescence resonance energy transfer pairs. Anal Biochem 2006 ; 356: 108-116.
11. Robers MB, Horton RA, Bercher MR, Vogel KW, Machleidt T.: High-throughput cellular assays for regulated posttranslational modifications. Anal Biochem 2008 ; 372: 189-197.
12. Ahuja N., Schwer B., Carobbio S., Waltregny D., North BJ, Castronovo V.,, et al. Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase. J Biol Chem 2007 ; 282: 33583-33592.
13. North BJ, Marshall BL, Borra MT, Denu JM, Verdin E.: The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase. Mol Cell 2003 ; 11: 437-444.