In vitro micronucleus assay for cigarette smoke using a whole smoke exposure system: A comparison of smoking regimens

Experimental and Toxicologic Pathology

Volumn 62, Issue 4, 2010, Pages 433-440

  Okuwa, Kosuke ^a   Tanaka, Masahiro ^a   Fukano, Yasuo ^a   Nara, Hidenori ^a   Nishijima, Yosuke ^a   Nishino, Tomoki ^a  

^a JAPAN TOBACCO INC   (Japan)

Author keywords

Air liquid interface; Cigarette smoke; CULTEX system; Direct exposure; Dosage; Gas vapor phase; Micronucleus assay; Whole smoke

Indexed keywords

CIGARETTE SMOKE;

ARTICLE; CELL CULTURE; CIGARETTE SMOKING; COMPARATIVE STUDY; CYTOTOXICITY; EXPOSURE; HAMSTER; IN VITRO STUDY; LUNG ALVEOLUS CELL; MICRONUCLEUS TEST; PARTICLE SIZE; SMOKING HABIT;

ANIMALS; CELL LINE; CELL SURVIVAL; CRICETINAE; CRICETULUS; EQUIPMENT DESIGN; LUNG; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MICRONUCLEUS TESTS; SMOKE; SMOKING; TOBACCO; TOXICITY TESTS;

CRICETULUS GRISEUS;

EID: 77953611607     PISSN: 09402993     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.etp.2009.06.002     Document Type: Article

References (32)

1. Andreoli C., Gigante D., Nunziata A. A review of in vitro methods to assess the biological activity of tobacco smoke with the aim of reducing the toxicity of smoke. Toxicol In Vitro 2003, 17:587-594.
2. Aufderheide M. An efficient approach to study the toxicological effects of complex mixtures. Exp Toxicol Pathol 2008, 60:163-180.
3. Aufderheide M., Gressmann H. Mutagenicity of native cigarette mainstream smoke and its gas/vapour phase by use of different tester strains and cigarettes in a modified Ames assay. Mutat Res 2008, 656:82-87.
4. Aufderheide M., Mohr U. CULTEX - a new system and technique for the cultivation and exposure of cells at the air/liquid interface. Exp Toxicol Pathol 1999, 51:489-490.
5. Baker R.R. " Smoke Chemistry" Tobacco Production, Chemistry and Technology 1999, Blackwell Science, London, UK.
6. Baker R.R., Massey E.D., Smith G. An overview of the effects of tobacco ingredients on smoke chemistry and toxicity. Food Chem Toxicol 2004, 42S:S53-S83.
7. Bird R.P., Draper H.H., Basrur P.K. Effect of malonaldehyde and acetaldehyde on cultured mammalian cells. Production of micronuclei and chromosomal aberrations. Mutat Res 1982, 101:237-246.
8. Bombick D.W., Ayres P.H., Putnam K., Bombick B.R., Doolittle D.J. Chemical and biological studies of a new cigarette that primarily heats tobacco. Part 3. In vitro toxicity of whole smoke. Food Chem Toxicol 1998, 36:191-197.
9. Bombick D.W., Bombick B.R., Ayres P.H., Putnam K., Avalos J., Borgerding M.F., et al. Evaluation of the genotoxic and cytotoxic potential of mainstream whole smoke and smoke condensate from a cigarette containing a novel carbon filter. Fundam Appl Toxicol 1997, 39:11-17.
10. Borgerding M., Klus H. Analysis of complex mixtures - cigarette smoke. Exp Toxicol Pathol 2005, 57:43-73.
11. Chen P.X., Moldoveanu S.C. Mainstream smoke chemical analyses for 2R4F Kentucky reference cigarette. Beitr Tabakforsch 2003, 20:448-458.
12. Clunes L.A., Bridges A., Alexis N., Tarran R. In vivo versus in vitro airway surface liquid nicotine levels following cigarette smoke exposure. J Anal Toxicol 2008, 32:201-207.
13. Counts M.E., Morton M.J., Laffoon S.W., Cox R.H., Lipowicz P.J. Smoke composition and predicting relationships for international commercial cigarettes smoked with three machine-smoking conditions. Regul Toxicol Pharmacol 2005, 41:185-227.
14. Diabaté S., Mülhopt S., Paur H.R., Krug H.F. The response of a co-culture lung model to fine and ultrafine particles of incinerator fly ash at the air-liquid interface. Altern Lab Anim 2008, 36:285-298.
15. DeMarini D.M., Gudi R., Szkudlinska A., Rao M., Recio L., Kehl M., et al. Genotoxicity of 10 cigarette smoke condensates in four test systems: comparisons between assays and condensates. Mutat Res 2008, 650:15-29.
16. Foy J.W., Bombick B.R., Bombick D.W., Doolittle D.J., Mosberg A.T., Swauger J.E. A comparison of in vitro toxicities of cigarette smoke condensate from Eclipse cigarettes and four commercially available ultra low-" tar" cigarettes. Food Chem Toxicol 2004, 42:237-243.
17. Fukano Y., Ogura M., Eguchi K., Shibagaki M., Suzuki M. Modified procedure of a direct in vitro exposure system for mammalian cells to whole cigarette smoke. Exp Toxicol Pathol 2004, 55:317-323.
18. Fukano Y., Yoshimura H., Yoshida T. Heme oxygenase-1 gene expression in human alveolar epithelial cells (A549) following exposure to whole cigarette smoke on a direct in vitro exposure system. Exp Toxicol Pathol 2006, 57:411-418.
19. ISO Cigarettes - determination of nicotine in smoke condensates. Gas-chromatographic method 2000, ISO 10315, International Organization for Standardization. 2nd ed.
20. ISO Cigarettes - determination of water in smoke condensates. Part 1: Gas-chromatographic method 1999, ISO 10362-1, International Organization for Standardization. 2nd ed.
21. Massey E., Aufderheide M., Koch W., Lodding H., Pohlmann G., Windt H., et al. MN induction in V79 cells after direct exposure to whole cigarette smoke. Mutagenesis 1998, 13:145-149.
22. Matsushima T., Hayashi M., Matsuoka A., Ishidate M., Miura K.F., Shimizu H., et al. Validation study of the in vitro micronucleus test in a Chinese hamster lung cell line (CHL/IU). Mutagenesis 1999, 14:569-580.
23. Maunders H., Patwardhan S., Phillips J., Clack A., Richter A. Human bronchial epithelial cell transcriptome: gene expression changes following acute exposure to whole cigarette smoke in vitro. Am J Physiol Lung Cell Mol Physiol 2007, 292:L1248-L1256.
24. Mizusaki S., Takashima T., Tomaru K. Factors affecting mutagenic activity of cigarette smoke condensate in Salmonella typhimurium TA1538. Mutat Res 1977, 48:29-36.
25. Nakayama T., Kaneko M., Kodama M., Nagata C. Cigarette smoke induces DNA single-strand breaks in human cells. Nature 1985, 314:462-464.
26. Olivera D.S., Boggs S.E., Beenhouwer C., Aden J., Knall C. Cellular mechanisms of mainstream cigarette smoke-induced lung epithelial tight junction permeability changes in vitro. Inhal Toxicol 2007, 19(1):13-22.
27. Préfontaine D., Morin A., Jumarie C., Porter A. In vitro bioactivity of combustion products from 12 tobacco constituents. Food Chem Toxicol 2006, 445:724-738.
28. Rickert W.S., Trivedi A.H., Momin R.A., Wright W.G., Lauterbach J.H. Effect of smoking conditions and methods of collection on the mutagenicity and cytotoxicity of cigarette mainstream smoke. Toxicol Sci 2007, 96:285-293.
29. Roemer E., Stabbert R., Rustemeier K., Veltel D.J., Meisgen T.J., Reininghaus W., et al. Chemical composition, cytotoxicity and mutagenicity of smoke from US commercial and reference cigarettes smoked under two sets of machine smoking conditions. Toxicology 2004, 195:31-52.
30. Tewes F.J., Meisgen T.J., Veltel D.J., Roemer E., Patskan G. Toxicological evaluation of an electrically heated cigarette. Part 3: Genotoxicity and cytotoxicity of mainstream smoke. J Appl Toxicol 2003, 23:341-348.
31. Wolz L., Krause G., Scherer G., Aufderheide M., Mohr U. In vitro genotoxicity assay of sidestream smoke using a human bronchial epithelial cell line. Food Chem Toxicol 2002, 40:845-850.
32. Yager J.W., Eastmond D.A., Robertson M.L., Paradisin W.M., Smith M.T. Characterization of micronuclei induced in human lymphocytes by benzene metabolites. Cancer Res 1990, 50:393-399.