Rapid monitoring of recombinant antibody production by mammalian cell cultures using Fourier transform infrared spectroscopy and chemometrics

Biotechnology and Bioengineering

Volumn 106, Issue 3, 2010, Pages 432-442

  Sellick, Christopher A ^a   Hansen, Rasmus ^a   Jarvis, Roger M ^a   Maqsood, Arfa R ^a   Stephens, Gill M ^b   Dickson, Alan J ^a   Goodacre, Royston ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Chemometrics; Fourier transform infrared spectroscopy; Mammalian cell culture; Partial least squares regression; Principal components analysis; PyChem; Recombinant antibody production

Indexed keywords

CHEMOMETRICS; MAMMALIAN CELL CULTURE; PARTIAL LEAST SQUARES REGRESSION; PRINCIPAL COMPONENTS ANALYSIS; RECOMBINANT ANTIBODY PRODUCTION;

AMIDES; ANIMAL CELL CULTURE; ANTIBODIES; CELL CULTURE; DISCRIMINANT ANALYSIS; FORECASTING; FOURIER TRANSFORM INFRARED SPECTROSCOPY; FOURIER TRANSFORMS; GLUCOSE; MAMMALS; MONITORING; MULTIVARIANT ANALYSIS; REGRESSION ANALYSIS; SPECTRUM ANALYSIS; SUGAR (SUCROSE); WAVELET TRANSFORMS;

PRINCIPAL COMPONENT ANALYSIS;

AMIDE; GLUCOSE; LACTIC ACID; POLYSACCHARIDE; RECOMBINANT ANTIBODY; RECOMBINANT PROTEIN;

ACCURACY; ANTIBODY PRODUCTION; ANTIBODY TITER; ARTICLE; BIOREACTOR; CELL CULTURE; CELL CULTURE MONITORING; CHEMOMETRICS; CHO CELL; CULTURE MEDIUM; DISCRIMINANT ANALYSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; INFRARED SPECTROSCOPY; MAMMAL CELL; NONHUMAN; PARTIAL LEAST SQUARES REGRESSION; PRINCIPAL COMPONENT ANALYSIS;

ANIMALS; ANTIBODIES; BIOTECHNOLOGY; CELL LINE; CRICETINAE; CRICETULUS; CULTURE MEDIA; ENZYME-LINKED IMMUNOSORBENT ASSAY; MICE; RECOMBINANT PROTEINS; SPECTROSCOPY, FOURIER TRANSFORM INFRARED;

CRICETULUS GRISEUS; MAMMALIA; MURINAE;

EID: 77953598931     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22707     Document Type: Article

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