Quality control of Pulsatilla koreana based on the simultaneous determination of triterpenoidal saponins by HPLC-ELSD and principal component analysis

Phytochemical Analysis

Volumn 21, Issue 4, 2010, Pages 314-321

  Lee, Ki Yong ^a   Cho, Young Woong ^a   Park, Junghyun ^a   Lee, Dong Young ^a   Kim, Seung Hyun ^a   Kim, Young Choong ^a   Sung, Sang Hyun ^a  

^a Seoul National University   (South Korea)

Author keywords

HPLC ELSD; Principal component analysis; Pulsatilla koreana; Triterpenoidal saponins

Indexed keywords

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIGHT SCATTERING; METABOLITES; QUALITY CONTROL;

ANTI-ANGIOGENIC ACTIVITY; ANTI-TUMORS; CYTOTOXIC ACTIVITIES; HPLC-ELSD; HYPOGLYCAEMIC; NEUROPROTECTIVE ACTIVITY; PRINCIPAL-COMPONENT ANALYSIS; PULSATILLUM KOREANA; SIMULTANEOUS DETERMINATIONS; TRITERPENOIDAL SAPONIN;

PRINCIPAL COMPONENT ANALYSIS;

ACETONITRILE; ANEMOSIDE B4; CUSSOSAPONIN C; HEDERACOLCHISIDE E; PULSATILLA SAPONIN H; PULSATILLOSIDE E; SAPONIN DERIVATIVE; TRITERPENOID; UNCLASSIFIED DRUG; WATER; SAPONIN; TRITERPENE;

ACCURACY; ANALYTIC METHOD; ARTICLE; CALIBRATION; CARBON NUCLEAR MAGNETIC RESONANCE; CONCENTRATION RESPONSE; DRUG DETERMINATION; DRUG STRUCTURE; ELUTION; EVAPORATIVE LIGHT SCATTERING DETECTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PLANT ROOT; PRINCIPAL COMPONENT ANALYSIS; PROTON NUCLEAR MAGNETIC RESONANCE; PULSATILLA; PULSATILLA KOREANA; QUALITY CONTROL; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; PROCEDURES; VALIDATION STUDY;

PULSATILLA; PULSATILLA KOREANA;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; PRINCIPAL COMPONENT ANALYSIS; PULSATILLA; QUALITY CONTROL; SAPONINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRITERPENES;

EID: 77953494015     PISSN: 09580344     EISSN: 10991565     Source Type: Journal    
DOI: 10.1002/pca.1201     Document Type: Article

References (21)

1. Bang SC, Kim Y, Lee JH, Ahn BZ. 2005a. Triterpenoid saponins from the roots of Pulsatilla koreana. J Nat Prod 68: 268-272. (Pubitemid 40299822)
2. Bang SC, Lee JH, Song GY, Kim DH, Yoon MY, Ahn BZ. 2005b. Antitumor activity of Pulsatilla koreana saponins and their structure-activity relationship. Chem Pharm Bull 53: 1451-1454. (Pubitemid 41634215)
3. Chen CY, Qi LW, Li HJ, Li P, Yi L, Ma HM, Tang D. 2007. Simultaneous determination of iridoids, phenolic acids, flavonoids, and saponins in Flos Lonicerae and Flos Lonicerae Japonicae by HPLC-DAD-ELSD coupled with principal component analysis. J Sep Sci 30: 3181-3192.
4. 3. Acta Chimica Sinica 48: 501-505.
5. Dekanosidze GE, Dzhikiya OD, Vugal'ter, MM, Kemertelidze EP. 1984. Triterpene glycosides of Hedera colchica, structure of hederacholchiside E and F. Khim Prir Soedin 6: 747-750.
6. Glebko LI, Krasovskaj NP, Strigina LI, Ulanova KP, Denisenko VA, Dmitrenok PS. 2002. Triterpene glycosides from Pulsatilla chinensis. Russian Chemical Bulletin 51: 1945-1950.
7. Han CK, Choi WR, Oh KB. 2007a. Cognition-enhancing and neuroprotective effects of hederacolchiside-E from Pulsatilla koreana. Planta Med 73: 665-669.
8. Han CK, Park YH, Jin DQ, Hwang YK, Oh KB, Han JS. 2007b. SK-PC-B70M from Pulsatilla koreana improves scopolamine-induced impairments of memory consolidation and spatial working memory. Brain Res 1184: 254-259.
9. Harinantenaina L, Kasai R, Yamasaki K. 2002. Cussosaponins A-E, triterpene saponins from the leaves of Cussonia racemosa, a Malagasy endemic plant. Chem Pharm Bull 50: 1290-1293.
10. Hsu HY, Chen YP, Shen SJ, Hsu CS, Chen CC, Chang HC. 1986. Oriental Materia Medica. Oriental Healing Art Institute: China; 226-227.
11. Kang SS. 1989. Saponins from the roots of Pulsatilla koreana. Arch Pharm Res 12: 42-27.
12. Kim HJ, Kim HT, Bae CI, Oh GJ, Park SK, Chung SG, Cho EH. 1997. Studies on the hypoglycemic constituent of Pulsatillae radix (I). Yakhak Hoeji 41: 709-713.
13. Kim Y, Kim SB, You YJ, Ahn BZ. 2002. Deoxypodophyllotoxin; the cytotoxic and antiangiogenic component from Pulsatilla koreana. Planta Med 68: 271-274.
14. Lee MK, Ahn YM, Lee KR, Jung JH, Jung OS, Hong J. 2009. Development of a validated liquid chromatographic method for the quality control of Prunellae spica: determination of triterpenic acids. Anal Chim Acta 633: 271-277.
15. Li BY, Hu Y, Liang YZ, Xie PS, Du YP. 2004. Quality evaluation of fingerprints of herbal medicine with chromatographic data. Anal Chim Acta 514; 69-77. (Pubitemid 38649027)
16. Lian X, Li BG, Gao XP, Guan JF, Ding LS, Chen YZ. 2001. Bioactive triterpenoid saponins from Anemone davidii. Zhongcaoyao 32: 493-496.
17. Massart DL, Vandeginste BGM, Deming SN, Michotte Y, Kaufman L. 1998. Chemometrics: A Textbook, Vol. 2, Elsevier: Amsterdam.
18. Mimaki Y, Yokosuka A, Kuroda M, Hamanaka M, Sakuma C, Sashida Y. 2001. New bisdesmosidic triterpene saponins from the roots of Pulsatilla chinensis. J Nat Prod 64: 1226-1229.
19. Oleszek W, Bialy Z. 2006. Chromatographic determination of plant saponins - an update (2002-2005). J Chromatogr A 1112: 78-91.
20. Yan S, Luo G, Wang Y, Cheng Y. 2006. Simultaneous determination of nine components in Qingkailing injection by HPLC/ELSD/DAD and its application to the quality control. J Pharm Biomed Anal 40: 889-895.
21. Yoo HH, Lee SK, Lim SY, Kim Y, Kang MJ, Kim EJ, Park YH, Im GJ, Lee BY, Kim DH. 2008. LC-MS/MS method for determination of hederacolchiside E, a neuroactive saponin from Pulsatilla koreana extract in rat plasma for pharmacokinetic study. J Pharm Biomed Anal 48: 1425-1429.