Development of a real-time PCR assay for identification and quantification of the fish pathogen Francisella noatunensis subsp. orientalis

Diseases of Aquatic Organisms

Volumn 89, Issue 3, 2010, Pages 199-207

  Soto, Esteban ^a   Bowles, Kimberly ^b   Fernandez, Denise ^a   Hawke, John P ^a  

^a LOUISIANA STATE UNIVERSITY   (United States)

^b LOUISIANA STATE UNIVERSITY   (United States)

Author keywords

Francisella; QPCR; Tilapia

Indexed keywords

BACTERIAL DISEASE; BACTERIUM; CICHLID; GENETIC ANALYSIS; GENOME; IDENTIFICATION METHOD; LINEARITY; PATHOGENICITY; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REAL TIME; SENSITIVITY ANALYSIS; SIGNAL;

BACTERIA (MICROORGANISMS); FRANCISELLA; FRANCISELLA TULARENSIS; MARTES PENNANTI; NEGIBACTERIA; OREOCHROMIS; TILAPIA;

ANIMAL; ANIMAL DISEASE; ARTICLE; FISH DISEASE; FRANCISELLA; GENETICS; GRAM NEGATIVE INFECTION; ISOLATION AND PURIFICATION; MICROBIOLOGY; PHYLOGENY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TILAPIA;

ANIMALS; FISH DISEASES; FRANCISELLA; GRAM-NEGATIVE BACTERIAL INFECTIONS; PHYLOGENY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TILAPIA;

EID: 77953413377     PISSN: 01775103     EISSN: 16161580     Source Type: Journal    
DOI: 10.3354/dao02204     Document Type: Article

References (43)

1. Abril C, Nimmervoll H, Pilo P, Brodard I and others (2008) Rapid diagnosis and quantification of Francisella tularensis in organs of naturally infected common squirrel monkeys (Saimiri sciureus). Vet Microbiol 127:203-208
2. Balcázar JL, Vendrell D, de Blas I, Ruiz-Zarzuela I, Gironés O, Múzquiz JL (2007) Quantitative detection of Aeromonas salmonicida in fish tissue by real-time PCR using selfquenched, fluorogenic primers. J Med Microbiol 56:323-328
3. Barker JR, Klose KE (2007) Molecular and genetic basis of pathogenesis in Francisella tularensis. Ann NY Acad Sci 1105:138-159
4. Birkbeck TH, Bordevik M, Frøystad MK, Baklien Å (2007) Identification of Francisella sp. from Atlantic salmon, Salmo salar L., in Chile. J Fish Dis 30:505-507
5. Bode E, Hurtle W, Norwood D (2004) Real-time PCR assay for a unique chromosomal sequence of Bacillus anthracis. J Clin Microbiol 42:5825-5831
6. Brotcke A, Weiss DS, Kim CC, Chain P, Malfatti S, Garcia E, Monack DM (2006) Identification of MglA-regulated genes reveals novel virulence factors in Francisella tularensis. Infect Immun 74:6642-6655
7. de Bruin OM, Ludu JS, Nano FE (2007) The Francisella pathogenicity island protein IglA localizes to the bacterial cytoplasm and is needed for intracellular growth. BMC Microbiol 7:1-10
8. Espy MJ, Uhl JR, Sloan LM, Buckwalter SP and others (2006) Real-time PCR in clinical microbiology: applications for routine laboratory testing. Clin Microbiol Rev 19:165-256
9. Euzéby J (2009) List of new names and new combinations previously effectively, but not validly, published. Validation List No. 128. Int J Syst Evol Microbiol 59:1555-1556
10. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791
11. Fujita O, Tatsumi M, Tanabayashi K, Yamada A (2006) Development of a real time PCR assay for detection and quantification of Francisella tularensis. Jpn J Infect Dis 59:46-51
12. Getchell RG, Groocock GH, Schumacher VL, Grimmett SG, Wooster GA, Bowser PR (2007) Quantitative polymerase chain reaction assay for largemouth bass virus. J Aquat Anim Health 19:226-233
13. Golovliov I, Ericsson M, Sandstrom G, Tarnvik A, Sjostedt A (1997) Identification of proteins of Francisella tularensis induced during growth in macrophages and cloning of the gene encoding a prominently induced 23-kilodalton protein. Infect Immun 65:2183-2189
14. Griffin MJ, Wise DJ, Camus AC, Mauel MJ, Greenway TE, Pote LM (2008) A real-time polymerase chain reaction assay for the detection of the myxozoan parasite Henneguya ictaluri in channel catfish. J Vet Diagn Investig 20:559-566
15. Hsieh CY, Tung MC, Tu C, Chang CD, Tsai SS (2006) Enzootics of visceral granulomas associated with Francisella-like organism infection in tilapia (Oreochromis spp.). Aquaculture 254:129-138
16. Hsieh CY, Wu ZB, Tung MC, Tsai SS (2007) PCR and in situ hybridization for the detection and localization of a new pathogen Francisella-like bacterium (FLB) in ornamental cichlids. Dis Aquat Org 75:29-36
17. Kamaishi T, Fukuda Y, Nishiyama M, Kawakami H, Matsuyama T, Yoshinaga T, Oseko N (2005) Identification and pathogenicity of intracellular Francisella bacterium in three-line grunt Parapristipoma trilineatum. Fish Pathol 40:67-71
18. Kay W, Petersen BO, Duus JØ, Perry MB, Vinogradov E (2006) Characterization of the lipopolysaccharide and β-glucan of the fish pathogen Francisella victoria. FEBS J 273:3002-3013
19. Kidd L, Maggi R, Diniz P P V P, Hegarty B, Tucker M, Breitschwerdt E (2008) Evaluation of conventional and realtime PCR assays for detection and differentiation of spotted fever group Rickettsia in dog blood. Vet Microbiol 129:294-303
20. Kocagoz T, Saribas Z, Alp A (2005) Rapid determination of rifampin resistance in clinical isolates of Mycobacterium tuberculosis by real-time PCR. J Clin Microbiol 43:6015-6019 (Pubitemid 43100653)
21. Lai XH, Golovliov I, Sjostedt A (2004) Expression of IglC is necessary for intracellular growth and induction of apoptosis in murine macrophages by Francisella tularensis. Microb Pathog 37:225-230
22. Lauriano CM, Barker JR, Yoon SS, Nano FE, Arulanandam BP, Hassett DJ, Klose KE (2004) MglA regulates transcription of virulence factors necessary for Francisella tularensis intraamoebae and intramacrophage survival. Proc Natl Acad Sci USA 101:4246-4249
23. Mauel MJ, Soto E, Moralis JA, Hawke J (2007) A piscirickettsiosis-like syndrome in cultured Nile tilapia in Latin America with Francisella spp. as the pathogenic agent. J Aquat Anim Health 19:27-34
24. Mikalsen J, Colquhoun DJ (2009) Francisella asiatica sp. nov. isolated from farmed tilapia (Oreochromis sp.) and elevation of Francisella philomiragia subsp. noatunensis to species rank as Francisella noatunensis comb. nov., sp. nov. Int J Syst Evol Microbiol, doi:10.1099/ijs.0.002139-0
25. Mikalsen J, Olsen AB, Tengs T, Colquohoun DJ (2007) Francisella philomiragia subsp. noatunensis subsp. nov., isolated from farmed Atlantic cod (Gadus morhua L.). Int J Syst Evol Microbiol 57:1960-1965
26. Nano FE, Schmerk C (2007) The Francisella pathogenicity island. Ann NY Acad Sci 1105:122-137
27. Nano FE, Zhang N, Cowley SC, Klose KE and others (2004) A Francisella tularensis pathogenicity island required for intramacrophage growth. J Bacteriol 186:6430-6436
28. Nylund A, Ottem KF, Watanabe K, Karlsbakk E, Krossøy B (2006) Francisella sp. (family Francisellaceae) causing mortality in Norwegian cod (Gadus morhua) farming. Arch Microbiol 185:383-392
29. Olsen AB, Mikalsen J, Rode M, Alfjorden A and others (2006) A novel systemic granulomatous inflammatory disease in farmed atlantic cod, Gadus morhua L., associated with a bacterium belonging to the genus Francisella. J Fish Dis 29:307-311
30. Ostland VE, Stannard JA, Creek JJ, Hedrick RP, Ferguson HW, Carlberg JM, Westerman ME (2006) Aquatic Francisella-like bacterium associated with mortality of intensively cultured hybrid striped bass Morone chrysops × M. saxatilis. Dis Aquat Org 72:135-145
31. Ottem KF, Nylund A, Karlsbakk E, Friis-Møller A, Krossøy B, Knappskog D (2007) New species in the genus Francisella (Gammaproteobacteria; Francisellaceae); Francisella piscicida sp. nov. isolated from cod (Gadus morhua). Arch Microbiol 188:547-550
32. Ottem KF, Nylund A, Isaksen TE, Karlsbakk E, Bergh O (2008) Occurrence of Francisella piscicida in farmed and wild Atlantic cod, Gadus morhua L., in Norway. J Fish Dis 31:525-534
33. Ottem KF, Nylund A, Karlsbakk E, Friis-Møller A, Kamaishi T (2009) Elevation of Francisella philomiragia subsp. noatunensis Mikalsen et al. (2007) to Francisella noatunensis comb. nov. [syn. Francisella piscicida Ottem et al. (2008) syn. nov.] and characterization of Francisella noatunensis subsp. orientalis subsp. nov., two important fish pathogens. J Appl Microbiol 106:1231-1243
34. Panangala VS, Shoemaker CA, Klesius PH (2007) TaqMan real-time polymerase chain reaction assay for rapid detection of Flavobacterium columnare. Aquac Res 38:508-517
35. Petersen JM, Schriefer ME (2005) Tularemia: emergence/re-emergence. Vet Res 36:455-467
36. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406-425
37. Santic M, Molmeret M, Klose KE, Jones S, Kwaik YA (2005) The Francisella tularensis pathogenicity island protein IglC and its regulator MglA are essential for modulating phagosome biogenesis and subsequent bacterial escape into the cytoplasm. Cell Microbiol 7:969-979
38. Soto E, Hawke J, Fernandez D, Morales JA (2009a) Francisella sp., an emerging pathogen of tilapia (Oreochromis niloticus) in Costa Rica. J Fish Dis 32:713-722
39. Soto E, Fernandez D, Hawke J (2009b) Attenuation of the fish pathogen Francisella sp. by mutation of the iglC gene. J Aquat Anim Health 21:140-149
40. Suzuki K, Sakai DK (2007) Real-time PCR for quantification of viable Renibacterium salmoninarum in chum salmon Oncorhynchus keta. Dis Aquat Org 74:209-223
41. Takahashi T, Tamura M, Takahashi SN, Matsumoto K and others (2007) Quantitative nested real-time PCR assay for assessing the clinical course of tuberculous meningitis. J Neurol Sci 255:69-76
42. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596-1599
43. Tomaso H, Scholz HC, Neubauer H, Al DS and others (2007) Real-time PCR using hybridization probes for the rapid and specific identification of Francisella tularensis subspecies tularensis. Mol Cell Probes 21:12-16