PCR and in situ hybridization for the detection and localization of a new pathogen Francisella-like bacterium (FLB) in ornamental cichlids

Diseases of Aquatic Organisms

Volumn 75, Issue 1, 2007, Pages 29-36

  Hsieh, Chia Yu ^a   Wu, Zong Bing ^a   Tung, Ming Chen ^a   Tsai, Shinn Shoung ^a  

^a NATIONAL PINGTUNG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Taiwan)

Author keywords

Cichlid; FLB; Francisella like bacterium; In situ hybridization; Ornamental fish; PCR

Indexed keywords

BACTERIAL DISEASE; DETECTION METHOD; DNA; GENETIC ANALYSIS; HISTOPATHOLOGY; HYBRIDIZATION; ORNAMENTAL SPECIES; PERCIFORM; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); CICHLIDAE; FRANCISELLA; TILAPIA;

EID: 34248386092     PISSN: 01775103     EISSN: None     Source Type: Journal    
DOI: 10.3354/dao075029     Document Type: Article

References (25)

1. Barrett DM, Faigel DO, Metz DC, Montone K, Furth EE (1997) In situ hybridization for Helicobacter pylori in gastric mucosal biopsy specimens: quantitative evaluation of test performance in comparison with the CLO test and thiazine stain. J Clin Lab Anal 11:374-379
2. Bashir MS, Lewis FA, Quirke P, Lee A, Dixon MF (1994) In situ hybridization for the identification of Helicobacter pylori in paraffin wax embedded tissue. J Clin Pathol 47:862-864
3. Brown C (1998) In situ hybridization with riboprobes: an overview for veterinary pathologists. Vet Pathol 35:159-167
4. Campbell LA, Patton DL, Moore DE, Cappuccio AL, Mueller BA, Wang SP (1993) Detection of Chlamydia trachomatis deoxyribonucleic acid in women with tubal infertility. Fertil Steril 59:45-50
5. Chae JS, Kim MS, Madigan J (2002) Detection of Neorickettsia (Ehrlichia) in tissues of mice experimentally infected with cercariae of trematodes by in situ hybridization. Vet Microbiol 88:233-243
6. Chen RS, Chao CB (1994) Outbreak of a disease caused by rickettsia-like organism in cultured tilapias in Taiwan. Fish Pathol 29:61-7
7. Chen SC, Tung MC, Chen SP, Tsai JF, Wang PC, Chen RS, Lin SC, Adams A (1994) Systematic granulomas caused by a rickettsia-like organism in Nile tilapia, Oreochronuics niloticus (L.), from southern Taiwan. J Fish Dis 17:591-599
8. Doggett PE, Blattner FR (1986) Personal access to sequence databases on personal computers. Nucleic Acids Res 14:611-619
9. Gebhart CJ, McOrist S, Lawson GHK, Collins JE, Ward GE (1994) Specific in situ hybridization of the intracellular organism of porcine proliferative enteropathy. Vet Pathol 31:462-467
10. Gencay M (1997) Chlamydia trachomatis detected in human placenta. J Clin Pathol 50:852-855
11. Gumus B, Sengil AZ, Solak M, Fistik T, Alibey E, Cakmak EA, Yeter M (1997) Evaluation of non-invasive clinical samples in chronic Chlamydial prostatitis by using in situ hybridization. Stand J Ural Nephrol 31:449-451
12. Hayashi Y, Watanabe J, Nakata K, Fukayama M, Ikeda H (1990) A novel diagnostic method of Pneumocystis carinii. In situ hybridization of ribosomal ribonucleic acid with biotinylated oligonucleotide probes. Lab Invest 63:576-580
13. Hsieh CY, Tung MC, Tu C, Chang CD, Tsai SS (2006) Enzootics of visceral granulomas associated with Francisella-like organism infection in tilapia (Oreochromis spp.). Aquaculture 254:129-138
14. Hsieh CY, Tung MC, Liu HJ, Tsai SS (2007) Isolation and identification of a new fish pathogen, Francisella-like bacterium (FLB) causing systemic granulomas in tilapias. J Fish Dis (in press)
15. Hulten K, Karttunen TJ, El-Zimaity HMT, Naser SA, Almashhrawi A, Graham DY, El-Zaatari FAK (2000) In situ hybridization method for studies of cell wall deficient M. paratuberculosis in tissue samples. Vet Microbiol 77:513-518
16. Jantos CA, Nesseler A, Waas W, Baumgartner W, Tillmanns H, Haberbosch W (1999) Low prevalence of Chlamydia pneumoniae in atherectomy specimens from patients with coronary heart disease. Clin Infect Dis 28:988-992
17. Komminoth P, Werner M (1997) Target and signal amplification: approaches to increase the sensitivity of in situ hybridization. Histochem Cell Biol 108:325-333
18. Kwon D, Chae C (1999) Detection and localization of Mycoplasma hyopneumoniae DNA in lungs from naturally infected pigs by in situ hybridization using a digoxigenin labeled probe. Vet Pathol 36:308-313
19. Lewis FA, Wells M (1992) Detection of virus in infected human tissue by in situ hybridization. In: Wilkinson DG (ed) In situ hybridization: a practical approach. Oxford University Press, Oxford, p 125-136
20. Loy JK, Dewhirst FE, Weber W, Frelier PF, Garbar TL, Tasca SI, Templeton JW (1996) Molecular phylogeny and in situ detection of the etiologic agent of necrotizing hepatopancreatitis in shrimp. Appl Environ Microbiol 62:3439-3445
21. Mauel MJ, Miller DL, Frazier K, Liggeet AD, Styer L, Montgomery-Brock D, Brock J (2003) Characterization of a piscirickettsiosis-like disease in Hawaiian tilapia. Dis Aquat Org 53:249-255
22. Nylund A, Ottem KF, Watanabe K, Karlsbakk E, Krossøy B (2006) Francisella sp. (family Francisellaceae) causing mortality in Norwegian cod (Gadus morhua) farming. Arch Microbiol 185:383-392
23. Olsen AB, Mikalsen J, Rode M, Alfjorden A, Hoel E, Straum-Lie K, Haldorsen R, Colquhoun DJ (2006) A novel systemic granulomatous inflammatory disease in farmed Atlantic cod, Gadus morhua L., associated with a bacterium belonging to the genus Francisella. J Fish Dis 29:307-311
24. Teifke JP, Hardt M, Weiss E (1994) Detection of bovine papillomavirus DNA in formalin-fixed and paraffin-embedded equine sarcoids by polymerase chain reaction and non-radioactive in situ hybridization. Eur J Vet Pathol 1:5-10
25. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673-4680