Real-time PCR for quantification of viable Renibacterium salmoninarum in chum salmon Oncorhynchus keta

Diseases of Aquatic Organisms

Volumn 74, Issue 3, 2007, Pages 209-223

  Suzuki, Kunio ^a   Sakai, D K ^b  

^a Eastern Hokkaido Inland Water Fisheries Section   (Japan)

^b TOKYO UNIVERSITY OF AGRICULTURE   (Japan)

Author keywords

Bacterial kidney disease; Chum salmon; msa gene mRNA; Real time PCR; Renibacterium salmoninarum; Viability

Indexed keywords

BACTERIAL DISEASE; GENE; POLYMERASE CHAIN REACTION; REAL TIME; SALMONID; VIABILITY;

BACTERIA (MICROORGANISMS); ONCORHYNCHUS KETA; ONCORHYNCHUS MASOU; ONCORHYNCHUS NERKA; PISCES; RENIBACTERIUM SALMONINARUM; SALMONIDAE;

EID: 34247490678     PISSN: 01775103     EISSN: None     Source Type: Journal    
DOI: 10.3354/dao074209     Document Type: Article

References (66)

1. Alifano P, Bruni CB, Calromango MS (1994) Control of mRNA processing and decay in prokaryotes. Genetica 94:157-172
2. Armstrong RD, Martin SW, Evelyn TPT, Hicks B, Dorward WJ, Ferguson HW (1989) A field evaluation of an indirect fluorescent antibody-based broodstock screening test used to control the vertical transmission of Renibacterium salmoninarum in chinook salmon (Oncorhynchus tshawytscha). Can J Vet Res 53:385-389
3. Arraiano CM, Yancey SD, Kushner SR (1988) Stabilization of discrete mRNA breakdown products in ams pnp rnb multiple mutants of Escherichia coli K-12. J Bacteriol 170: 4625-4633
4. Austin B, Embley TM, Goodfellow M (1983) Selective isolation of Renibacterium salmoninarum. FEMS Microbiol Lett 17: 111-114
5. Belasco JG (1993) mRNA degradation in prokaryotic cells: an overview. In: Belasco J, Braorman G (eds) Control of messenger RNA stability. Academic Press, San Diego, CA, p 3-12
6. Belasco JG, Nilsson G, von Gabain A, Cohen SN (1986) The stability of E. coli transcripts is dependent on determinants localized to specific mRNA segments. Cell 46:245-251
7. Benediktsdóttir E, Gudmundsdóttir S, Helgason S (1991) Incubation time for the cultivation of Renibacterium salmoninarum from Atlantic salmon, Salmo salar L., broodfish. J Fish Dis 14:97-102
8. Birch L, Dawson CE, Cornett JH, Keer JT (2001) A comparison of nucleic acid amplification techniques for the assessment of bacterial viability. Lett Appl Microbiol 33:296-301
9. Brown LL, Iwama GK, Evelyn TPT, Nelson WS, Levine RP (1995) Bacterial species other than Renibacterium salmoninarum cross-react with antisera against R. salmoninarum but are negative for the p57 gene of R. salmoninarum as detected by the polymerase chain reaction. Dis Aquat Org 21:227-231.
10. Chase DM, Pascho RJ (1998) Development of a nested polymerase chain reaction for amplification of a sequence of the p57 gene of Renibacterium salmoninarum that provides a highly sensitive method for detection of the bacterium in salmonid kidney. Dis Aquat Org 34:223-229
11. Chase DM, Elliott DG, Pascho RJ (2006) Detection and quantification of Renibacterium salmoninarum DNA in salmonid tissues by real-time quantitative polymerase chain reaction analysis. J Vet Invest 18:375-380.
12. Chien MS, Gilbert TL, Huang C, Landolt ML, O'Hara PJ, Winton JR (1992) Molecular cloning and sequence analysis of the gene of the salmonid fish pathogen Renibacterium salmoninarum. FEMS Microbiol Lett 96:259-266
13. Cook M, Lynch WH (1999) A sensitive nested transcriptase PCR assay to detect viable cells of the fish pathogen Renibacterium salmoninarum in Atlantic salmon (Salmo salar L.). Appl Environ Microbiol 65:3042-3047
14. Cvitanich JD (1994) Improvements in the direct fluorescent antibody technique for the detection, identification, and quantification of Renibacterium salmoninarum in salmonid kidney smears. J Aquat Anim Health 6:1-12
15. Deere D, Porter J, Pickup RW, Edwards C (1996) Survival of cells and DNA of Aeromonas salmonicida released into aquatic microcosms. J Appl Bacteriol 81:309-318
16. Elliott DG, Barila TV (1987) Membrane filtration-fluorescent antibody staining procedure for detecting and quantifying Renibacterium salmoninarum in coelomic fluid of chinook salmon (Oncorhynchus tshawytscha). Can J Fish Aquat Sci 44:206-210
17. Evelyn TPT (1977) An improved growth medium for the kidney disease bacterium and some notes on using the medium. Bull Off Int Epiz 87:511-513
18. Evelyn TPT, Ketcheson JE, Prosperi-Porta L (1981) The clinical significance of immunofluorescent-based diagnoses of the bacterial kidney disease carrier. Fish Pathol 15:293-300
19. Evenden AJ, Grayson TH, Gilpin ML, Munn CB (1993) Renibacterium salmoninarum and bacterial kidney disease - the unfinished jigsaw. Annu Rev Fish Dis 3:87-104
20. Fryer JL, Sanders JE (1981) Bacterial kidney disease of salmonid fish. Annu Rev Microbiol 35:273-298
21. Grayson TH, Cooper LF, Atienzar FA, Knowles MR, Gilpin ML (1999) Molecular differentiation of Renibacterium salmoninarum isolates from worldwide locations. Appl Environ Microbiol 65:961-968
22. Grayson TH, Cooper LF, Wrathmell AB, Roper J, Evenden AJ, Gilpin ML (2002) Host responses to Renibacterium salmoninarum and specific components of the pathogen reveal the mechanisms of immune suppression and activation. Immunology 106:273-283
23. Griffiths SG, Olivier G, Fildes J, Lynch WH (1991) Comparison of western blot, direct fluorescent antibody and dropplate culture methods for the detection of Renibacterium salmoninarum in Atlantic salmon {Salmo salar L.). Aquaculture 97:117-129
24. Griffiths SG, Liska K, Lynch WH (1996) Comparison of kidney tissue and ovarian fluid from broodstock Atlantic salmon for detection of Renibacterium salmoninarum, and use of SKDM broth culture with Western blotting to increase detection in ovarian fluid. Dis Aquat Org 24:3-9
25. Gudmundsdóttir S, Benediktsdóttir E, Helgason S (1993) Detection of Renibacterium salmoninarum in salmonid kidney samples: a comparison of results using double-sandwich ELISA and isolation on selective medium. J Fish Dis 16:185-195
26. Gueimonde M, Tölkkö S, Korpimäki T, Salminen S (2004) New real-time PCR procedure for quantification of bifidobacteria in human fecal samples. Appl Environ Microbiol 70: 4165-4169
27. Hein I, Lehner A, Rieck P, Klein K, Brandl E, Wagner M (2001) Comparison of different approaches to quantify Staphylococcus aureus cells by real-time quantitative PCR and application of this technique for examination of cheese. Appl Environ Microbiol 67:3122-3126
28. Hellyer TJ, DesJardin LE, Hehman GL, Cave MD, Eisennach KD (1999) Quantitative analysis of mRNA as a marker for viability of Mycobacterium tuberculosis. J Clin Microbiol 37:290-295.
29. Izumi S, Wakabayashi H (2000) Sequencing of gyrB and their application in the identification of Flavobacterium psychrophilum by PCR. Fish Pathol 35:93-94
30. Josephson KL, Gerba CP, Pepper IL (1993) Polymerase chain reaction of nonviable bacterial pathogens. Appl Environ Microbiol 59:3513-3515
31. Jou NT, Yoshimori RB, Mason GR, Louie JS (1997) Single-tube, nested, reverse transcriptase PCR for detection of viable Mycobacterium tuberculosis. J Clin Microbiol 35: 1161-1165
32. Keer JT, Birch T (2003) Molecular methods for the assessment of bacterial viability. J Microbiol Methods 53:175-183
33. Kimura T, Yoshimizu M (1981) Rapid method for detection of bacterial kidney disease (BKD) by coagglutination of antibody sensitized protein A-containing Staphylococci. Bull Jpn Soc Sci Fish 47:1173-1183
34. Kimura T, Yoshimizu M, Hara T (1987) Diagnostic procedures for the bacterial kidney disease of salmonids. In: Suisan-zosyoku-sousyo, 35. Jpn Resour Conserv Assoc, Tokyo (In Japanese with English summary)
35. Kushner SR (1996) mRNA decay. In: Neidhardt FC, Curtiss R, Ingraham JL, Lin ECC, Low KB, Magasanik B, Reznikoff WS, Riley R, Schaechter M, Umbarger HE (eds) Escherichia coli and Salmonella: Cellular and molecular biology, vol. 1, Am Soc Microbiol, Washington, DC, p 849-860
36. Lovely JE, Cabo C, Griffith SG, Lynch WH (1994) Detection of Renibacterium salmoninarum infection on asymptomatic Atlantic salmon. J Aquat Anim Health 6:126-132
37. Masters CI, Shallcross JA, Mackey BM (1994) Effect of stress treatments on the detection of Listeria monocytogenes and enterotoxigenic Escherichia coli by the polymerase chain reaction. J Appl Bacteriol 77:73-79
38. McIngvale SC, Elhanafi D, Drake MA (2002) Optimization of reverse transcription PCR to detect viable shiga-producing Escherichia coli. Appl Environ Microbiol. 68: 799-806.
39. McIntosh D, Meaden PG, Austin B (1996) A simplified PCR-based method for the detection of Renibacterium salmoninarum using preparations of rainbow trout (Oncorhynchus mykiss, Walbaum) lymphocytes. Appl Environ Microbiol 62:3929-3932
40. Miriam A, Griffith SG., Lovely JE, Lynch WH (1997) PCR and probe-PCR assays to monitor broodstock Atlantic salmon (Salmo salar L.) ovarian fluid and kidney tissue for presence of DNA of the fish pathogen. J Clin Microbiol 35: 1322-1326
41. Nogva HK, Rudi K, Naterstad K, Holck A, Lillehaug D (2000) Application of 5′-nuclease PCR for quantitative detection of Listeria monocytogenes in pure culture, water, skim milk, and unpasteurized whole milk. Appl Environ Microbiol 66: 4266-4271
42. O'Farrell CL, Strom MS (1999) Differential expression of the virulence-associated protein p57 and characterization of its duplicated gene msa in virulent and attenuated strains of Renibacterium salmoninarum. Dis Aquat Org 38:115-123
43. O'Hara EB, Chekanova JA, Ingle CA, Kushner ZR, Peters E, Kushner SR (1995) Polyadenylation helps regulate mRNA decay in Escherichia coli. Proc Natl Acad Sci USA 92: 1807-1811
44. Olea I, Bruno DW, Hastings TS (1993) Detection of Renibacterium salmoninarum in naturally infected Atlantic salmon, Salmo salar L., and rainbow trout, Oncorhynchus mykiss (Walbaum) using an enzyme-linked immunosorbent assay. Aquaculture 116:99-110
45. Olivier G, Griffiths SG, Fildes J, Lynch WH (1992) The use of western blot and electroimmunotransfer blot assays to monitor bacterial kidney disease in experimentally challenged Atlantic salmon, Salmo salar L. J Fish Dis 15:229-241
46. Pascho RJ, Mulcahy D (1987) Enzyme-linked immunosorbent assay for a soluble antigen of Renibacterium salmoninarum, the causative agent of salmonid bacterial kidney disease. Can J Fish Aquat Sci 44:183-191
47. Pascho RJ, Chase D, McKibben CL (1998) Comparison of the membrane-filtration fluorescent antibody test, the enzyme-linked immunosorbent assay, and the polymerase chain reaction to detect Renibacterium salmoninarum in salmonid ovarian fluid. J Vet Diagn Invest 10:60-66
48. Pascho RJ, Elliott DG, Streufert JM (1991) Brood stock segregation of spring chinook salmon Oncorhynchus tshawytscha by use of the enzyme-linked immunosorbent assay (ELISA) and the fluorescent antibody technique (FAT) affects the prevalence and levels of Renibacterium salmoninarum infection in progeny. Dis Aquat Org 12:25-40
49. Powell M, Overturf K, Hogge C, Johnson K (2005) Detection of Renibacterium salmoninarum in chinook salmon, Oncorhynchus tshawytscha (Walbaum), using quantitative PCR. J Fish Dis 28:615-622
50. Rauhut R, Klug G (1999) mRNA degradation in bacteria. FEMS Microbiol Rev 23:353-370.
51. Requena T, Burton J, Matsuki T, Munro K, Simon MA, Tanaka R, Watanabe K, Tannock GW (2002) Identification, detection, and enumeration of human bifidobacterium species by PCR targeting the transaldolase gene. Appl Environ Microbiol 68:2420-2427
52. Rhodes LD, Alison MC, Deinhand RK (2004) Identification of a third msa gene in Renibacterium salmoninarum and the associated virulence phenotype. Appl Environ Microbiol 70:6488-6494
53. Rhodes LD, Durkin C, Nance SL, Rice CA (2006) Prevalence and analysis of Renibacterium salmoninarum infection among juvenile Chinook salmon Oncorhynchus tshawytscha in North Puget Sound. Dis Aquat Org 71:179-190
54. Rockey DO, Gilkey LL, Wiens GD, Kaattari SL (1991) Monoclonal antibody-based analysis of the Renibacterium salmoninarum p57 protein in spawning chinook and coho salmon. J Aquat Anim Health 3:23-30
55. Rodríguez-Lázaro D, Hernández M, Scortti M, Esteve T, Vázquez-Boland JA, Pla M (2004) Quantitative detection of Listeria monocytogenes and Listeria innocua by real-time PCR: assessment of hly, jap, and lin02483 targets and AmpliFluor technology. Appl Environ Microbiol 70: 1366-1377
56. Sakai DK, Nagata N, Iwama T, Tamiya Y, Ito Y, Atoda M (1986) Attempt to control BKD by dietary modification and erythromycin chemotherapy in hatchery-reared masu salmon Oncorhynchus masou Brevoort. Bull Jpn Soc Sci Fish 52: 1141-1147
57. Sakai M, Atsuta S, Kobayashi M (1991) Susceptibility of five salmonid fishes to Renibacterium salmoninarum. Fish Pathol 26:159-160
58. Sheridan GE, Masters CI, Shallcross JA, Mackey BM (1998) Detection of mRNA by transcription-PCR as an indicator of viability in Escherichia coli cells. Appl Environ Microbiol 64:1313-1318
59. Starliper CE (1996) Genetic diversity of North American isolates of Renibacterium salmoninarum. Dis Aquat Org 27: 207-213
60. Steege DA (2000) Emerging features of mRNA decay in bacteria. RNA (NY) 6:1079-1090
61. Toyama T, Kita-Tsukamoto K, Wakabayashi H (1994) Identification of Cytophaga psychrophila by PCR 16S ribosomal RNA. Fish Pathol 29:271-275
62. Turuga PSD, Wiens GD, Kaattari SL (1987) Analysis of Renibacterium salmoninarum antigen production in situ. Fish Pathol 22:209-214
63. Von Gabain A, Belasco JG, Scottel JL, Chang ACY, Cohen SN (1983) Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts. Proc Natl Acad Sci USA 80:653-657
64. Wakabayashi H, Egusa S (1974) Characteristics of myxobacteria associated with some freshwater fish diseases in Japan. Bull Jpn Soc Sci Fish 13:751-757
65. Wiens GD, Kaattari SL (1989) Monoclonal antibody analysis of common surface protein(s) of Renibacterium salmoninarum. Fish Pathol 24:1-7
66. 139-to-Glu substitution in the Renibacterium salmoninarum virulence-associated protein p57 results in antigenic variation and is associated with enhanced p57 binding to Chinook salmon leukocytes. Appl Environ Microbiol 68: 3969-3977