Production of a novel pectin lyase from Bacillus pumilus (P9), Purification and characterisation and fruit juice application

Romanian Biotechnological Letters

Volumn 15, Issue 2, 2010, Pages 5167-5176

  Nadaroǧlu, Hayrunnisa ^a   Taşkin, Esen ^a   Adigüzel, Ahmet ^a   Güllüce, Medine ^a   Demir, Nazan ^a  

^a ATATÜRK UNIVERSITY   (Turkey)

Author keywords

Bacillus pumilus (P9); Juice Clarification; Pectin; Pectin lyase; Purification

Indexed keywords

BACILLUS PUMILUS;

EID: 77953355503     PISSN: 12245984     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (32)

1. Hoondal, G.S., Tiwari, R.P., Tewari, R., Dahiya, N., Beg, Q.K. 2002. Microbial alkaline pectinases and their industrial applications: a review. Appl. Microbiol. Biotechnol. 59: 409-418.
2. Alkorta, I., Garbisu, C., Llama, M.J., Serra, J.L. 1998. Industrial applications of pectic enzymes: a review. Process Biochem. 33: 21-28.
3. Kashyap, D.R., Vohra, P.K., Chopra, S., Tewari, R. 2001. Applications of pectinases in the commercial sector: a review. Bioresource Technol. 77: 215-227.
4. Takao, M., Nakaniwa, T., Yoshikawa, K., Terashita, T., Sakai, T. 2000. Purification and characterization of thermostable pectate lyase with protopectinase activity from thermophilic Bacillus sp. TS 47. Biosci. Biotechnol. Biochem. 64: 2360-2367.
5. Kapoor, M., Beg, Q.K., Bhushan, B., Dadhich, K.S., Hoondal, G.S. 2000. Production and partial purification and characterization of a thermo-alkali stable polygalacturonase from Bacillus sp. MGcp-2. Process Biochem. 36: 467-473.
6. Hayashi, K., Inoue, Y., Shiga, M., Sato, S., Takano, R., Hirayae, K., Hibi, T., Hara, S. 1997. Pectinolytic enzymes from Pseudomonas marginalis MAFF03-01173. Phytochemistry 45: 1359-1363.
7. Patil, S.R., Dayanand, A. 2006. Optimization of process for the production of fungal pectinases from deseeded sunflower head in submerged and solid-state conditions. Bioresource Technol. 97: 2340-2344.
8. Huang, L.K., Mahoney, R.R. 1999. Purification and characterization of an endo-polygalacturonase from Verticillum albo-atrum. J. Appl. Microbiol. 86: 145-156.
9. Beg, Q.K., Bhushan, B., Kapoor, M., Hoondal, G.S. 2000. Production and characterization of thermostable xylanase and pectinase from a Streptomyces sp. QG-11-3. J. Ind. Microbiol. Biotechnol. 24: 396-402.
10. Blancoa, P., Sieiroa, C., Villaa, T.G. 1999. Production of pectic enzymes in yeasts. FEMS Microbiol. Lett. 175: 1-9.
11. Jayani, R.S., Saxena, S. , Gupta, R. 2005. Microbial pectinolytic enzymes: A review. Process Biochem. 40: 2931-2944.
12. Rai, P., Majumdar, G.C., Das, Gupta, S., DE, S. 2004. Optimizing pectinase usage in pretreatment of mosambi juice for clarification by response surface methodology. J. Food Eng. 64: 397-403.
13. Sunnotel, O., Nigam, P. 2002. Pectinolytic activity of bacteria isolated from soil and two fungal strains during submerged fermentation. World J. Microbiol. Biotechnol. 18: 835-839.
14. Taragano, V.M., Pilosof, A.M.R. 1999. Application of Doehlert designs for water activity, pH, and fermentation time optimization for Aspergillus niger pectinolytic activities production in solid-state and submerged fermentation. Enzym. Microb. Tech. 25: 411-419.
15. Cao, J., Zheng, L., Chen, S. 1992. Screening of pectinase producer from alkalophilic bacteria and study on its potential application in degumming of rammie. Enzym. Microbial. Technol. 14: 1013-1016
16. Sharma HSS. 1987. Enzymatic degradation of residual non cellulosic polysaccharides present on dew retted flax fibers. Appl. Microbiol. Biotechnol. 26: 358-362.
17. Tanabe, H., Yoshihara, K., Tamura, K., Kobayashi, Y., Akamatsu, I., Niyomwan, N., Footrakul, P. 1987. Pretreatment of pectic waste water from orange canning process by an alkalophilic Bacillus sp. J. Ferm. Technol. 65: 243-246.
18. Afifi, A.F., Fawzi, E.M., Foaad, M.A. 2002. Purification and characterization of a pectin lyase produced by Curvularia inaequalis NRRL 13884 on orange peels waste, solid state culture. Ann. Microbiol. 52: 287-297
19. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-54.
20. Nedjma, M., Hoffmann, N., Belarbi, A. 2001. Selective and sensitive detection of pectin lyase activity using a colorimetric test: application to the screening of microorganisms possessing pectin lyase activity. Anal. Biochem. 291: 290-296.
21. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature 227: 680-685.
22. Whitaker, J.R. 1963. Determination of molecular weight of proteins by gel filtration on sephadex. Anal. Chem. 35: 1950-1953.
23. Soraes, M.M.C.N., DA Silva, R., Carmona, E.C., Gomes, E. 2001. Pectinolytic enzyme production by Bacillus species and their potential application on juice extraction. World J. Microbiol. Biotechnol. 17: 79-82.
24. Kim, J.C., Kim, H.Y., Choi, Y.J. 1998. Production and characterization of acid-stable pectin lyase from Bacillus sp. PN33. J. Microbiol. Biotechnol. 8: 353-360.
25. Alana, A., Llama, M.J., Serra, J.L. 1991. Purification and some properties of the pectin lyase from Penicillium italicum. FEBS Lett. 280: 335-340.
26. OBI, S.K.C., Moneke, N.A. 1985. Pectin lyase and polygalacturonase of Aspergillus niger pathogenic for yam tubers (Diascorea rotundata). Int. J. Food Microbiol. 1: 277-289.
27. Dosanjh, N., Hoondal, G.S. 1996. Production of constitutive, thermostable, hyperactive exopectinase from Bacillus GK-8. Biotechnol. Lett. 18: 1435-1438.
28. Kashyap, D.R., Chandra, S., Kaul, A., Tewari, R. 2000. Production, purifi0cation and characterization of pectinase from a Bacillus sp. DT7. World J. Microbiol. Biotechnol. 17: 277-282.
29. Hamdy, H.S. 2005. Purification and characterization of the pectin lyase produced by Rhizopus oryzae grown on orange peels. Ann. Microbiol., 55: 205-211.
30. Martin, N., De Souza, S.R., DA Silva, R., Gomae, E. 2004. Pectinase production by fungal strains in solid-state fermentation using agro-industrial bioproduct. Braz. Arch. Biol. Tech. 47: 813-819.
31. Chen, W.C., Hsieh, H.J., Tseng, T.C. 1998. Purification and characterization of a pectin lyase from Pythium splendens infected cucumber fruits. Botanical Bulletin of Academia Sinica 39: 181-186.
32. Pilnik, W., Voragen, A.G.J. 1993. Pectic enzymes in fruit juice manufacture. In: Nagodawithama, T., and Reed, G. eds., Enzymes in Food Processi+ng. New York, Academic Press, pp. 363-399.