Purification and characterization of an endo-polygalacturonase from Verticillium albo-atrum

Journal of Applied Microbiology

Volumn 86, Issue 1, 1999, Pages 145-156

  Huang, L K ^a   Mahoney, R R ^a  

^a UNIVERSITY OF MASSACHUSETTS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALKYLATION; ELECTROPHORESIS; ENZYME ACTIVITY; HYDROLYSIS; IONIC STRENGTH; PURIFICATION; REACTION RATES;

BASIC PROTEINS; CELL WALLS; CULTURE FILTRATE; DEGREE OF METHYLATIONS; ISO-ELECTRIC POINTS; PLANT PATHOGEN; POLYGALACTURONASE; POLYGALACTURONIC ACID; RELATIVE REACTION RATES; TOMATO CELLS;

SUBSTRATES;

FUNGAL PROTEIN; GALACTURONIC ACID; PECTIN; POLYGALACTURONASE; POLYGALACTURONIC ACID;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SUBSTRATE; FUNGUS; HYDROLYSIS; METHYLATION; NONHUMAN; VERTICILLIUM ALBO ATRUM;

EID: 0032954512     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2672.1999.00645.x     Document Type: Article

References (32)

1. Albersheim, P. and Anderson, A.J. (1971) Proteins from plant cell walls inhibit polygalacturonases secreted by plant pathogens. Proceedings of the National Academy of Science, USA 68, 1815-1819.
2. Ashworth, L.J. Jr, Huisan, O.C., Harper, D.M. and Stromberg, L.K. (1979) Verticillium wilt disease of tomato: influence of inoculum density and root extension upon disease severity. Phytopathology 69, 490-492.
3. Bateman, D.F. and Millar, R.L. (1966) Pectic enzymes in tissue degradation. Annual Review of Phytopathology 4, 119-146.
4. Bitter, T. and Muir, H.M. (1962) A modified uronic acid carbazole reaction. Analytical Biochemistry 4, 330-334.
5. Brown, A.E. and Adikaram, N.K.B. (1983) A role of pectinase and protease inhibitors in fungal rot development in tomato fruits. Phytopathologie Zeitschrift 106, 239-251.
6. Carder, J.H., Hignett, R.C. and Swinburne, T.R. (1987) Relationship between the virulence of hop isolates of Verticillium alboatrum and their in vitro secretion of cell wall degradation enzymes. Physiological and Molecular Plant Pathology 31, 441-452.
7. Cervone, F., Hahn, M.G., De Lorenzo, G., Darvill, A. and Albersheim, P. (1989) Host-pathogen interactions. XXXIII. A plant protein converts a fungal pathogenesis factor into an elicitor of plant defense responses. Plant Physiology 90, 542-548.
8. Collmer, A. and Keen, N.T. (1986) The role of pectic enzymes in plant pathogenesis. Annual Review of Phytopathology 24, 383-409.
9. Demain, A.L. and Phaff, H.J. (1954) The preparation of tetragalacturonic acid. Archives of Biochemistry and Biophysics 51, 114-121.
10. Doerner, K.C. and White, B.A. (1990) Detection of glycoproteins separated by nondenaturing polyacrylamide gel electrophoresis using the periodic acid-Schiff stain. Analytical Biochemistry 187, 147-150.
11. Dubray, G. and Bezard, G. (1982) A highly sensitive periodic acid-silver stain for 1,2-diol groups of glycoproteins and polysaccharides in polyacrylamide gels. Analytical Biochemistry 119, 325-329.
12. Hahn, M.G., Darvill, A.G. and Albersheim, P. (1981) Host-pathogen interaction. XIX. The endogenous elicitor, a fragment of a plant cell wall polysaccharide that elicits phytoalexin accumulation in soybeans. Plant Physiology 68, 1161-1169.
13. Jin, D.F. and West, C.A. (1984) Characteristics of galacturonic acid oligomers as elicitors of casbene synthetase activity in castor bean seedlings. Plant Physiology 74, 989-992.
14. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
15. Lineweaver, H. and Ballou, G.A. (1945) The effect of cations of the activity of alfalfa pectinesterase (pectase). Archives of Biochemistry 6, 373-387.
16. Lowry, O.H., Rosebrough, N.J.,Farr, A.L. and Randall, R.J. (1954) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265-275.
17. Luh, B.S., Leonard, S.J. and Phaff, H.J. (1956) Hydrolysis of pectic materials and oligouronides by tomato polygalacturonase. Food Research 21, 448-455.
18. Malca, I., Erwin, D.C., Moje, W. and Jones, B. (1966) Effect of pH and carbon and nitrogen sources on the growth of Verticillium albo-atrum. Phytopathology 56, 401-406.
19. Mussell, H.W. and Strouse, B. (1972) Characterization of two polygalacturonases produced by Verticillium albo-atrum. Canadian Journal of Biochemistry 50, 625-632.
20. Nasuno, S. and Starr, M.P. (1966) Polygalacturonase of Erminia carolovora. Journal of Biological Chemistry 241, 5298-5306.
21. Nelson, N. (1944) A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry 153, 375-380.
22. Rauschen, D. (1979) Glycoprotein detection in polyacrylamide gel with thymol and sulfuric acid. Analytical Biochemistry 99, 474-476.
23. Rexová-Benková, L. (1973) The size of the substrate-binding site of an Aspergillus niger extracellular endopolygalacturonase. European Journal of Biochemistry 39, 109-115.
24. Roboz, E., Barratt, R.W. and Tatum, E.L. (1952) Breakdown of pectic substances by a new enzyme from Neurospora. Journal of Biological Chemistry 195, 459-471.
25. Shields, R. and Burnett, W. (1960) Determination of protein-bound carbohydrate in serum by a modified anthrone method. Analytical Chemistry 32, 885-886.
26. Somogyi, M. (1952) Notes on sugar determination. Journal iof Biological Chemistry 195, 19-23.
27. Stotz, H.U., Contos, J.J.A., Powell, A.L.T., Bennett, A.B. and Labavitch, J.M. (1994) Structure and expression of an inhibitor of fungal polygalacturonases from tomato. Plant Molecular Biology 25, 607-617.
28. Talboys, P.W. (1984) Damage, symptoms and crop loss caused by vascular pathogens. In Plant Diseases : Infection, Damage and Loss ed. Wood, R.K.A. and Jellis, G.J. pp. 171-187. Oxford : Blackwell Scientific Publications.
29. Wang, M.C. and Keen, N.T. (1970) Purification and characterization of endopolygalacturonase from Verticillium albo-actrum. Archives of Biochemistry and Biophysics 141, 749-757.
30. Warrilow, A.G.S. and Jones, M.G. (1995) Different forms of tomato pectinesterase have different kinetic properties. Phytochemistry 39, 277-282.
31. Wood, R.K.S. (1961) Verticillium wilt of tomatoes - the role of pectic and cellulolytic enzymes. Annals of Applied Biology 49, 120-139.
32. Wrigley, C.W. (1971) Gel electrofocusing. Methods in Enzymology 22, 559-564.