Selection of Pichia pastoris strains expressing recombinant immunoglobulin G by cell surface labeling

Journal of Immunological Methods

Volumn 358, Issue 1-2, 2010, Pages 66-74

  Lin, Song ^a   Shen, Zheng ^a   Zha, Dongxing ^a   Sharkey, Nathan ^a   Prinz, Bianka ^a   Hamilton, Stephen ^a   Pavoor, Tej Venkatachalam ^a   Bobrowicz, Beata ^a   Shaikh, Seemab S ^a   Rittenhour, Alissa M ^a   Potgieter, Thomas I ^a   Bobrowicz, Piotr ^a   Stadheim, Terrance A ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Cell surface labeling; Fluorescence activated cell sorting (FACS); Pichia pastoris antibody expressing strain; Strain productivity improvement

Indexed keywords

RECOMBINANT ANTIBODY; RECOMBINANT IMMUNOGLOBULIN G ANTIBODY; UNCLASSIFIED DRUG;

ANTIBODY PRODUCTION; ANTIBODY TITER; ARTICLE; BIOTECHNOLOGICAL PRODUCTION; CELL LABELING; CELL SURFACE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; FERMENTATION; FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENCE ANALYSIS; FUNGAL STRAIN; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; RELIABILITY; STRAIN IMPROVEMENT;

ANIMALS; ANTIBODIES; BIOREACTORS; FLOW CYTOMETRY; GOATS; HUMANS; IMMUNOGLOBULIN G; MEMBRANE PROTEINS; MICROSCOPY, CONFOCAL; PICHIA; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; STAINING AND LABELING;

PICHIA; PICHIA PASTORIS;

EID: 77953291188     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2010.03.007     Document Type: Article

References (19)

1. Akselband Y., Moen O.T., McGrath P. Isolation of rare isotype switch variants in hybridoma cell lines using an agarose gel microdrop-based protein secretion assay. Assay Drug Dev. Technol. 2003, 1:619.
2. Borth N., Zeyda M., Kunert R., Katinger H. Efficient selection of high producing subclones during gene amplification of recombinant Chinese hamster ovary cells by flow cytometry and cell sorting. Biotechnol. Bioeng. 2000, 71(4):266.
3. Brezinsky S.C.G., Chiang G.G., Szilvasi A., Mohan S., Shapiro R.I., MacLean A., Sisk W., Thill G. A simple method for enriching populations of transfected CHO cells for cells of higher specific productivity. J. Immunol. Meth. 2003, 277:141.
4. Browne S.M., Al-Rubeai M. Selection methods for high-producing mammalian cell lines. Trends Biotechnol. 2007, 25(9):425.
5. Cherlet M., Kromenaker S.J., Sirenc F. Surface IgG content of murine hybridomas: direct evidence for variation of antibody secretion rates during the cell cycle. Biotechnol. Bioeng. 5 1995, 47(5):535.
6. Cregg J.M., Russel K.A. Transformation. Pichia protocols 1998, 27. Humana Press, Totowa, NJ. 1st edn. D.R. Higgins, J.M. Cregg (Eds.).
7. Gerngross T.U. Advances in the production of human therapeutic proteins in yeasts and filamentous fungi. Nat. Biotechnol. 2004, 22:1409.
8. Gray F., Kenny J.S., Dunne J.F. Secretion capture and report web: use of affinity derivatized agarose microdroplets for the selection of hybridoma cells. J. Immunol. Meth. 1995, 182:155.
9. Hamilton S.R., Davidson Robert C., Sethuraman Natarajan, Nett Juergen H., Jiang Youwei, Rios Sandra, Bobrowicz Piotr, Stadheim Terrance A., Li Huijuan, Choi Byung-Kwon, Hopkins Daniel, Wischnewski Harry, Roser Jessica, Mitchell Teresa, Strawbridge Rendall R., Hoopes Jack, Wildt Stefan, Gerngross Tillman U. Humanization of yeast to produce complex terminally sialylated glycoproteins. Science 2006, 313(8):1441.
10. Holmes P., Al-Rubeai M. Improved cell line development by a high throughput affinity capture surface display technique to select for high secretors. J. Immunol. Meth. 1999, 230(1-2):141.
11. Li H., Sethuraman N., Stadheim T.A., Zha D., Prinz B., Ballew N., Bobrowicz P., Choi B.K., Cook W.J., Cukan M., Houston-Cummings N.R., Davidson R., Gong B., Hamilton S.R., Hoopes J.P., Jiang Y., Kim N., Mansfield R., Nett J.H., Rios S., Strawbridge R., Wildt S., Gerngross T.U. Optimization of humanized IgGs in glycoengineered Pichia pastoris. Nat. Biotechnol. 2006, 24:210.
12. Manz R., Assenmacher M., Pflüger E., Miltenyi S., Radbruch A. Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix. PNAS 1995, 92(6):1921.
13. Marder P., Maciak R.S., Fouts R.L., Baker R.S., Starling J.L. Selective cloning of hybridoma cells for enhanced immunoglobulin production using flow cytometric cell sorting and automated laser nephelometry. Cytometry 1990, 11:498.
14. Mattanovich D., Borth N. Applications of cell sorting in biotechnology. Microb. Cell Fact. 2006, 5:12.
15. McKinney K.L., Dilwith R., Belfort G. Manipulation of heterogeneous hybridoma cultures for overproduction of monoclonal antibodies. Biotechnol. Prog. 1991, 7:445.
16. Pichler J., Hesse F., Wieser M., Kunert R., Galosy S.S., Mott E.J., Borth N. A study on the temperature dependency and time course of the cold capture antibody secretion assay. J. Biotechnol. 2009, 141(2009):80.
17. Powell K.T., Weaver J.C. Gel microdroplets and flow cytometry: rapid determination of antibody secretion by individual cells within a cell population. Nat. Biotechnol. 1990, 8(4):333.
18. Rakestraw J.A., Baskaran A.R., Wittrup K.D. A flow cytometric assay for screening improved heterologous protein secretion in yeast. Biotechnol. Prog. 2006, 22:1200.
19. Sen S., Hu W.-S., Srienc F. Flow cytometric study of hybridoma cell culture: correlation between cell surface fluorescence and IgG production rate. Enzyme Microb. Technol. 1990, 12:571.