Heterologous expression of diverse barley XTH genes in the yeast Pichia pastoris

Plant Biotechnology

Volumn 27, Issue 3, 2010, Pages 251-258

  Kaewthai, Nomchit ^a   Harvey, Andrew J ^b   Hrmova, Maria ^b   Brumer, Harry ^a   Ezcurra, Ines ^a   Teeri, Tuula T ^a   Fincher, Geoffrey B ^b  

^a ALBANOVA UNIVERSITY CENTER   (Sweden)

^b UNIVERSITY OF ADELAIDE   (Australia)

Author keywords

Pichia pastoris; Plant protein expression; XEH; XET; Xyloglucan endo transglycosylase hydrolase (XTH) genes

Indexed keywords

EID: 77953215212     PISSN: 13424580     EISSN: 13476114     Source Type: Journal    
DOI: 10.5511/plantbiotechnology.27.251     Document Type: Article

References (41)

1. Arrowsmith DA, de Silva J (1995) Characterization of 2 Tomato Fruit-Expressed cDNAs Encoding Xyloglucan Endo-Transglycosylase. Plant Mol Biol 28: 391-403
2. Baumann MJ, Eklöf JM, Michel G, Kallas ÅM, Teeri TT, Czjzek M, Brumer H (2007) Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: Biological implications for cell wall metabolism. Plant Cell 19: 1947-1963
3. Becnel J, Natarajan M, Kipp A, Braam J (2006) Developmental expression patterns of Arabidopsis XTH genes reported by transgenes and Genevestigator. Plant Mol Biol 61: 451-467
4. Boettner M, Steffens C, von Mering C, Bork P, Stahl U, Lang C (2007) Sequence-based factors influencing the expression of heterologous genes in the yeast Pichia pastoris-A comparative view on 79 human genes. J Biotechnol 130: 1-10
5. Bolot S, Abrouk M, Masood-Quraishi U, Stein N, Messing J, Feuillet C, Salse J (2009) The 'inner circle' of the cereal genomes. Curr Opin Plant Biol 12: 119-125
6. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254
7. Campbell P, Braam J (1999) In vitro activities of four xyloglucan endotransglycosylases from Arabidopsis. Plant J 18: 371-382
8. Cantarel BL, Coutinho PM, Rancurel C, Bernard T, Lombard V, Henrissat B (2009) The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics. Nucleic Acids Res 37: D233-D238
9. Cao PJ, Bartley LE, Jung KH, Ronald PC (2008) Construction of a rice glycosyltransferase phylogenomic database and identification of rice-diverged glycosyltransferases. Mol Plant 1: 858-877
10. Catala C, Rose JKC, York WS, Albersheim P, Darvill AG, Bennett AB (2001) Characterization of a tomato xyloglucan endotransglycosylase gene that is down-regulated by auxin in etiolated hypocotyls. Plant Physiol 127: 1180-1192
11. Chanliaud E, de Silva J, Strongitharm B, Jeronimidis G, Gidley MJ (2004) Mechanical effects of plant cell wall enzymes on cellulose/xyloglucan composites. Plant J. 38: 27-37
12. Cosgrove DJ (2005) Growth of the plant cell wall. Nat Rev Mol Cell Biol 6: 850-861
13. Daly R, Hearn MTW (2005) Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production. J Mol Recognit 18: 119-138
14. Davies GJ, Henrissat B (2002) Structural enzymology of carbohydrate-active enzymes: implications for the post-genomic era. Biochem. Soc Trans 30: 291-297
15. de Silva J, Jarman CD, Arrowsmith DA, Stronach MS, Chengappa S, Sidebottom C, Reid JSG (1993) Molecular characterization of a xyloglucan-specific endo-(1→4)-beta-D-glucanase (xyloglucan endotransglycosylase) from nasturtium seeds. Plant J 3: 701-711
16. Farrokhi N, Hrmova M, Burton RA, Fincher GB (2008) Heterologous and Cell Free Protein Expression Systems. Meth Mol Biol 513: 175-198
17. Fry SC, Smith RC, Renwick KF, Martin DJ, Hodge SK, Matthews KJ (1992) Xyloglucan endotransglycosylase, a new wallloosening enzyme activity from plants. Biochem J 282: 821-828
18. Geisler-Lee J, Geisler M, Coutinho PM, Segerman B, Nishikubo N, Takahashi J, Aspeborg H, Djerbi S, Master E, Andersson-Gunneras S, Sundberg B, Karpinski S, Teeri TT, Kleczkowski LA, Henrissat B, Mellerowicz EJ (2006) Poplar carbohydrateactive enzymes. Gene identification and expression analyses. Plant Physiol 140: 946-962
19. Gleba Y, Klimyuk V, Marillonnet S (2007) Viral vectors for the expression of proteins in plants. Curr Opin Biotechnol 18: 134-141
20. Henriksson H, Denman SE, Campuzano IDG, Ademark P, Master ER, Teeri TT, Brumer H (2003) N-linked glycosylation of native and recombinant cauliflower xyloglucan endotransglycosylase 16A. Biochem J 375: 61-73
21. Henrissat B, Coutinho PM, Davies GJ (2001) A census of carbohydrate-active enzymes in the genome of Arabidopsis thaliana. Plant Mol Biol 47: 55-72
22. Holmquist M, Tessier DC, Cygler M (1997) High-level production of recombinant Geotrichum candidum lipases in yeast Pichia pastoris. Protein Expr Purif 11: 35-40
23. Hrmova M, Farkaš V, Harvey AJ, Lahnstein J, Wischmann B, Kaewthai N, Ezcurra I, Teeri TT, Fincher GB (2009) Substrate specificity and catalytic mechanism of a xyloglucan xyloglucosyl transferase HvXET6 from barley (Hordeum vulgare L.). FEBS J 276: 437-456
24. Hrmova M, Farkaš V, Lahnstein J, Fincher GB (2007) A barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans. J Biol Chem 282: 12951-12962
25. Hrmova M, Fincher GB (2009) Functional genomics and structural biology in the definition of gene function. Meth Mol Biol 513: 199-227
26. Johansson P, Brumer H, Baumann MJ, Kallas ÅM, Henriksson H, Denman SE, Teeri TT, Jones TA (2004) Crystal structures of a poplar xyloglucan endotransglycosylase reveal details of transglycosylation acceptor binding. Plant Cell 16: 874-886
27. Kallas ÅM, Piens K, Denman SE, Henriksson H, Fäldt J, Johansson P, Brumer H, Teeri TT (2005) Enzymatic properties of native and deglycosylated hybrid aspen (Populus tremula x tremuloides) xyloglucan endotransglycosylase 16A expressed in Pichia pastoris. Biochem J 390: 105-113
28. Liepman AH, Wightman R, Geshi N, Turner SR, Scheller HV (2010) Arabidopsis- a powerful model system for plant cell wall research. Plant J 61: 1107-1121
29. Michel G, Chantalat L, Duee E, Barbeyron T, Henrissat B, Kloareg B, Dideberg O (2001) The kappa-carrageenase of P. carrageenovora features a tunnel-shaped active site: A novel insight in the evolution of clan-B glycoside hydrolases. Structure 9: 513-525
30. Minic Z (2008) Physiological roles of plant glycoside hydrolases. Planta 227: 723-740
31. Mohand FA, Farkaš V (2006) Screening for hetero-transglycosylating activities in extracts from nasturtium (Tropaeolum majus). Carbohyd Res 341: 577-581
32. Oh MH, Romanow WG, Smith RC, Zamski E, Sasse J, Clouse SD (1998) Soybean BRU1 encodes a functional xyloglucan endotransglycosylase that is highly expressed in inner epicotyl tissues during brassinosteroid-promoted elongation. Plant Cell Physiol 39: 124-130
33. Plesha MA, Huang TK, Dandekar AM, Falk BW, McDonald KA (2009) Optimization of the bioprocessing conditions for scale-up of transient production of a heterologous protein in plants using a chemically inducible viral amplicon expression system. Biotechnol Prog 25: 722-734
34. Saladié M, Rose JKC, Cosgrove DJ, Catala C (2006) Characterization of a new xyloglucan endotransglucosylase/hydrolase (XTH) from ripening tomato fruit and implications for the diverse modes of enzymic action. Plant J 47: 282-295
35. Schröder R, Atkinson RG, Langenkamper G, Redgwell RJ (1998) Biochemical and molecular characterisation of xyloglucan endotransglycosylase from ripe kiwifruit. Planta 204: 242-251
36. Sreenivasulu N, Graner A, Wobus U (2008) Barley Genomics: An Overview. International Journal of Plant Genomics Article ID 486258: 13 pages. doi:10.1155/2008/486258
37. Streatfield SJ (2007) Approaches to achieve high-level heterologous protein production in plants. Plant Biotechnol J 5: 2-15
38. Toikkanen JH, Niku-Paavola ML, Bailey M, Immanen J, Rintala E, Elomaa P, Helariutta Y, Teeri TH, Fagerström R (2007) Expression of xyloglucan endotransglycosylases of Gerbera hybrida and Betula pendula in Pichia pastoris. J Biotechnol 130: 161-170
39. Van Sandt VST, Guisez Y, Verbelen JP, Vissenberg K (2006) Analysis of a xyloglucan endotransglycosylase/hydrolase (XTH) from the lycopodiophyte Selaginella kraussiana suggests that XTH sequence characteristics and function are highly conserved during the evolution of vascular plants. J Exp Bot 57: 2909-2922
40. Yokoyama R, Nishitani K (2001) A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of arabidopsis. Plant Cell Physiol 42: 1025-1033
41. Yokoyama R, Rose JKC, Nishitani K (2004) A surprising diversity and abundance of xyloglucan endotransglucosylase/hydrolases in rice. Classification and expression analysis. Plant Physiol 134: 1088-1099