Enzymatic properties of native and deglycosylated hybrid aspen (Populus tremula x tremuloides) xyloglucan endotransglycosylase 16A expressed in Pichia pastoris

Biochemical Journal

Volumn 390, Issue 1, 2005, Pages 105-113

  Kallas, Åsa M ^a   Piens, Kathleen ^a   Denman, Stuart E ^a,c   Henriksson, Hongbin ^a   Fäldt, Jenny ^a   Johansson, Patrik ^b   Brumer III, Harry ^a   Teeri, Tuula T ^a  

^a ALBANOVA UNIVERSITY CENTER   (Sweden)

^b UPPSALA UNIVERSITY   (Sweden)

^c CSIRO   (Australia)

Author keywords

Glycoside hydrolase family 16 (GH16); Hybrid aspen (populus tremula x tremuloides); Pichia pastoris; Protein glycosylation; Protein MS; Xyloglucan endotransglycosylase (XET)

Indexed keywords

CATALYSIS; ENZYMES; HYDROLYSIS; YEAST;

GLYCOSIDE HYDROLASE FAMILY 16; PROTEIN GLYCOSYLATION; PROTEIN MS;

DNA;

COMPLEMENTARY DNA; GLUCAN; GLYCOSIDASE; HYDROLASE; SIGNAL PEPTIDE; UNCLASSIFIED DRUG; XYLOGLUCAN ENDOTRANSGLYCOSYLASE;

ARTICLE; ASPEN; CATALYSIS; DEGLYCOSYLATION; ENZYME ISOLATION; GLYCOSYLATION; METHYLOTROPHIC BACTERIUM; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; WILD TYPE; YEAST;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BINDING SITES; ENZYME STABILITY; GENE EXPRESSION; GLYCOSYLTRANSFERASES; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; MUTATION; PICHIA; POPULUS; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN ISOFORMS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; TEMPERATURE;

PICHIA PASTORIS; POPULUS TREMULOIDES;

EID: 23944474570     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20041749     Document Type: Article

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