Real-time polymerase chain-reaction detection of pathogens is feasible to supplement the diagnostic sequence for urinary tract infections

BJU International

Volumn 106, Issue 1, 2010, Pages 114-120

  Lehmann, Lutz E ^a   Hauser, Stefan ^a   Malinka, Thomas ^a   Klaschik, Sven ^a   Stüber, Frank ^b   Book, Malte ^b  

^a UNIVERSITY OF BONN   (Germany)

^b UNIVERSITY OF BERN   (Switzerland)

Author keywords

Bacterial DNA; Pathogen detection; Real time PCR; Urinary tract infection; Urine culture

Indexed keywords

AGAR; BACTERIAL DNA;

ADULT; AGED; ARTICLE; BACTERIAL GROWTH; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; CRITICALLY ILL PATIENT; DIAGNOSTIC VALUE; DISEASE SEVERITY; FEASIBILITY STUDY; FEMALE; HUMAN; INTENSIVE CARE UNIT; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALE; MOLECULAR PROBE; OUTPATIENT DEPARTMENT; PILOT STUDY; PRIORITY JOURNAL; PROSPECTIVE STUDY; QUALITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; SENSITIVITY AND SPECIFICITY; UNIVERSITY HOSPITAL; URINARY TRACT INFECTION; URINE CULTURE;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; DNA PROBES; DNA, BACTERIAL; EPIDEMIOLOGIC METHODS; FEMALE; HUMANS; MALE; MIDDLE AGED; REAGENT KITS, DIAGNOSTIC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; URINARY TRACT INFECTIONS; YOUNG ADULT;

EID: 77953201935     PISSN: 14644096     EISSN: 1464410X     Source Type: Journal    
DOI: 10.1111/j.1464-410X.2009.09017.x     Document Type: Article

References (27)

1. Nicolle LE. Epidemiology of urinary tract infections. Infect Med 2001 18 : 153 162
2. Brun-Buisson C. The epidemiology of the systemic inflammatory response. Intensive Care Med 2000 26 (Suppl. 1 S64 74
3. Palmqvist E, Aspevall O, Burman E, Nordin G, Svahn A, Forsum U. Difficulties for primary health care staff in interpreting bacterial findings on a device for simplified urinary culture. Scand J Clin Lab Invest 2008 68 : 312 316
4. Corless CE, Guiver M, Borrow R, Edwards-Jones V, Fox AJ, Kaczmarski EB. Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCR. J Clin Microbiol 2001 39 : 1553 1558
5. Lehmann LE, Hunfeld KP, Emrich T et al. A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med Microbiol Immunol 2008 197 : 313 324
6. Hinata N, Shirakawa T, Okada H, Shigemura K, Kamidono S, Gotoh A. Quantitative detection of Escherichia coli from urine of patients with bacteriuria by real-time PCR. Mol Diagn 2004 8 : 179 184
7. Shigemura K, Shirakawa T, Okada H et al. Rapid detection and differentiation of Gram-negative and Gram-positive pathogenic bacteria in urine using TaqMan probe. Clin Exp Med 2005 4 : 196 201
8. Kass EH. Bacteriuria and the diagnosis of infections of the urinary tract; with observations on the use of methionine as a urinary antiseptic. AMA Arch Intern Med 1957 100 : 709 714
9. Chen Q, Book M, Fang X, Hoeft A, Stuber F. Screening of copy number polymorphisms in human beta-defensin genes using modified real-time quantitative PCR. J Immunol Methods 2006 308 : 231 240
10. Takeuchi T, Katsume A, Tanaka T et al. Real-time detection system for quantification of hepatitis C virus genome. Gastroenterology 1999 116 : 636 642 (Pubitemid 29106771)
11. Zoller L, Tobler L. [Comparison of culture count determination with the uricult pour-plate]. Med Lab (Stuttg) 1969 22 : 214 217
12. Duerden BI, Moyes A. Comparison of laboratory methods in the diagnosis of urinary tract infection. J Clin Pathol 1976 29 : 286 291
13. European Confederation of Laboratory Medicine. European Urinalysis Guidelines. Scand J Clin Lab Invest 2000 60 (Suppl. 231 1 96
14. Aspevall O, Forsum U, Kjerstadius T, Hallander H. Evaluation of two methods for improving quality of diagnosis of bacteriuria by culture in primary healthcare. Scand J Clin Lab Invest 2000 60 : 387 393
15. Mignini L, Carroli G, Abalos E et al. Accuracy of diagnostic tests to detect asymptomatic bacteriuria during pregnancy. Obstet Gynecol 2009 113 : 346 352
16. Harbarth S, Garbino J, Pugin J, Romand JA, Lew D, Pittet D. Inappropriate initial antimicrobial therapy and its effect on survival in a clinical trial of immunomodulating therapy for severe sepsis. Am J Med 2003 115 : 529 535
17. Struelens MJ. The emerging power of molecular diagnostics: towards improved management of life-threatening infection. Intensive Care Med 2001 27 : 1696 1698
18. Elhanan G, Sarhat M, Raz R. Empiric antibiotic treatment and the misuse of culture results and antibiotic sensitivities in patients with community-acquired bacteraemia due to urinary tract infection. J Infect 1997 35 : 283 288
19. Kumar A, Roberts D, Wood K et al. Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med 2006 34 : 1589 1596
20. Kuti EL, Patel AA, Coleman CI. Impact of inappropriate antibiotic therapy on mortality in patients with ventilator-associated pneumonia and blood stream infection: a meta-analysis. J Crit Care 2008 23 : 91 100
21. Vromen MdV, Knols A, Stobberingh EE. Antimicrobial resistance patterns in urinary isolates from nursing home residents. Fifteen years of data reviewed. J Antimicrob Chemother 1999 44 : 113 116
22. Kahlmeter G. Prevalence and antimicrobial susceptibility of pathogens in uncomplicated cystitis in Europe. The ECO SENS Study. Int J Antimicrob Agents 2003 22 (Suppl. 2 49 52
23. Larinkari U, Rautio M. Evaluation of a new dipslide with a selective medium for the rapid detection of beta-glucuronidase-positive Escherichia coli. Eur J Clin Microbiol Infect Dis 1995 14 : 606 609
24. Aspevall O, Kjerstadius T, Lindberg L, Hallander H. Performance of Uricult Trio assessed by a comparison method and external control panels in primary healthcare. Scand J Clin Lab Invest 2000 60 : 381 386
25. Maes N, Magdalena J, De Rottiers SGY, Struelens MJ. Evaluation of a triplex PCR assay to discriminate Staphylococcus aureus from coagulase-negative Staphylococci and determine methicillin resistance from blood cultures. J Clin Microbiol 2002 40 : 1514 1517
26. Saager B, Rohde H, Timmerbeil B et al. Molecular characterisation of linezolid resistance in two vancomycin-resistant (VanB) Enterococcus faecium isolates using pyrosequencing. Eur J Clin Microbiol Infect Dis 2008 27 : 873 878
27. Westh H, Lisby G, Breysse F et al. Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis. Clin Microbiol Infect 2009 15 : 544 551