Molecular characterisation of linezolid resistance in two vancomycin-resistant (VanB) Enterococcus faecium isolates using Pyrosequencing™

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 27, Issue 9, 2008, Pages 873-878

  Saager, B ^a   Rohde, H ^b   Timmerbeil, B S ^a   Franke, G ^b   Pothmann, W ^b   Dahlke, J ^b   Scherpe, S ^b   Sobottka, I ^b   Heisig, P ^a   Horstkotte, M A ^b,c  

^a UNIVERSITY OF HAMBURG   (Germany)

^b UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^c Bioscientia Center for Human Genetics   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CILASTATIN PLUS IMIPENEM; CIPROFLOXACIN; DAPTOMYCIN; FLUCONAZOLE; GENTAMICIN; LINEZOLID; METRONIDAZOLE; PIPERACILLIN; RIBOSOME RNA; RNA 23S; SULBACTAM; TETRACYCLINE; VANCOMYCIN;

ADULT; ALLELE; ANTIBIOTIC RESISTANCE; ARTICLE; CASE REPORT; ENTEROCOCCUS FAECIUM; FEMALE; GENE MUTATION; HUMAN; HUMAN CELL; HUMAN TISSUE; MOLECULAR MODEL; PERITONITIS; PHENOTYPE; PRIORITY JOURNAL; PYROSEQUENCING; QUANTITATIVE ANALYSIS; QUANTITATIVE DIAGNOSIS; RELIABILITY; RNA GENE; SINGLE NUCLEOTIDE POLYMORPHISM; WILD TYPE;

ACETAMIDES; ADULT; ANTI-BACTERIAL AGENTS; DRUG RESISTANCE, MULTIPLE, BACTERIAL; ELECTROPHORESIS, GEL, PULSED-FIELD; ENTEROCOCCUS FAECIUM; FEMALE; GENES, BACTERIAL; GENES, RRNA; GRAM-POSITIVE BACTERIAL INFECTIONS; HUMANS; LEUKEMIA, MYELOGENOUS, CHRONIC, BCR-ABL POSITIVE; MICROBIAL SENSITIVITY TESTS; MUCOSITIS; OXAZOLIDINONES; PANCREATITIS, ACUTE NECROTIZING; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; SEQUENCE ANALYSIS, DNA; VANCOMYCIN; VANCOMYCIN RESISTANCE;

EID: 49649108541     PISSN: 09349723     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10096-008-0514-6     Document Type: Article

References (15)

1. DL Shinabarger KR Marotti RW Murray AH Lin EP Melchior SM Swaney DS Dunyak WF Demyan JM Buysse 1997 Mechanism of action of oxazolidinones: effects of linezolid and eperezolid on translation reactions Antimicrob Agents Chemother 41 10 2132 2136
2. J Brauers M Kresken D Hafner PM Shah German Linezolid Resistance Study Group 2005 Surveillance of linezolid resistance in Germany, 2001-2002 Clin Microbiol Infect 11 1 39 46
3. MA Kainer RA Devasia TF Jones BP Simmons K Melton S Chow J Broyles KL Moore AS Craig W Schaffner 2007 Response to emerging infection leading to outbreak of linezolid-resistant enterococci Emerg Infect Dis 13 7 1024 1030
4. J Prystowsky F Siddiqui J Chosay DL Shinabarger J Millichap LR Peterson GA Noskin 2001 Resistance to linezolid: characterization of mutations in rRNA and comparison of their occurrences in vancomycin-resistant enterococci Antimicrob Agents Chemother 45 7 2154 2156
5. SH Marshall CJ Donskey R Hutton-Thomas RA Salata LB Rice 2002 Gene dosage and linezolid resistance in Enterococcus faecium and Enterococcus faecalis Antimicrob Agents Chemother 46 10 3334 3336
6. S Dutka-Malen S Evers P Courvalin 1995 Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR J Clin Microbiol 33 1 24 27
7. S Dutka-Malen S Evers P Courvalin 1995 Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR J Clin Microbiol 33 5 1434 Erratum
8. R Kariyama R Mitsuhata JW Chow DB Clewell H Kumon 2000 Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin-resistant enterococci J Clin Microbiol 38 8 3092 3095
9. Clinical and Laboratory Standards Institute (CLSI) (2006) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Seventh edition. Approved Standard M7-A7. CLSI, Wayne, PA
10. Clinical and Laboratory Standards Institute (CLSI) (2007) Performance standards for antimicrobial susceptibility testing. Seventeenth informational supplement. Approved Standard M100-S17. CLSI, Wayne, PA
11. HA Elsner I Sobottka HH Feucht E Harps C Haun D Mack R Ganschow R Laufs PM Kaulfers 2000 Nosocomial outbreak of vancomycin-resistant Enterococcus faecium at a German university pediatric hospital Int J Hyg Environ Health 203 2 147 152
12. M Ronaghi 2001 Pyrosequencing sheds light on DNA sequencing Genome Res 11 1 3 11
13. A Sinclair C Arnold N Woodford 2003 Rapid detection and estimation by pyrosequencing of 23S rRNA genes with a single nucleotide polymorphism conferring linezolid resistance in Enterococci Antimicrob Agents Chemother 47 11 3620 3622
14. S Swoboda S Fritz ME Martignoni RA Feldhues T Hoppe-Tichy MW Buchler HK Geiss 2005 Varying linezolid susceptibility of vancomycin-resistant Enterococcus faecium isolates during therapy: a case report J Antimicrob Chemother 56 4 787 789
15. N Woodford L Tysall C Auckland MW Stockdale AJ Lawson RA Walker DM Livermore 2002 Detection of oxazolidinone-resistant Enterococcus faecalis and Enterococcus faecium strains by real-time PCR and PCR-restriction fragment length polymorphism analysis J Clin Microbiol 40 11 4298 4300