Role of cytology and polymerase chain reaction based detection of Pneumocystis jirovecii infection in bronchoalveolar lavage fluid

Acta Cytologica

Volumn 54, Issue 3, 2010, Pages 296-302

  Gupta, Rashmi ^a   Iyer, Venkateswaran K ^a   Mirdha, Bijay Ranjan ^a   Guleria, Randeep ^a   Kumar, Lalit ^a   Agarwal, Sanjay Kumar ^a  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Bronchoalveolar lavage fluid; Pneumocystis pneumonia; Polymerase chain reaction

Indexed keywords

DNA; GLYCOPROTEIN;

ARTICLE; CELL POPULATION; CLINICAL TRIAL; CONTROLLED STUDY; CRYPTOCOCCUS; CYTOLOGY; DNA EXTRACTION; GENE; HUMAN; HUMAN CELL; INFLAMMATORY CELL; LUNG ALVEOLUS MACROPHAGE; LUNG LAVAGE; LYMPHOCYTE; MAJOR CLINICAL STUDY; MICROSCOPY; MIXED INFECTION; NEUTROPHIL; OBSERVATIONAL STUDY; PNEUMOCYSTIS JIROVECI; PNEUMONIA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROSPECTIVE STUDY; SAMPLE; SCREENING; SMEAR;

EID: 77953194840     PISSN: 00015547     EISSN: 19382650     Source Type: Journal    
DOI: 10.1159/000325038     Document Type: Article

References (28)

1. Stringer JR, Beard CB, Miller RF, Wakefield AE: A new name (Pneumocystis jirovecii) for Pneumocystis from humans. Emerg Infect Dis 2002;8:891-896
2. Saito K, Nakayamada S, Nakano K, Tokunaga M, Tsujimura S, Nakatsuka K, Adachi T, Tanaka Y: Detection of Pneumocystis carinii by DNA amplification in patients with connective tissue diseases: Re-evaluation of clinical features of P. carinii pneumo nia in rheumatic diseases. Rheumatology (Oxf) 2004;43:479-485
3. Wakefield AE, Pixley FJ, Banerji S, Sinclair K, Miller RF, Moxon ER, Hopkin JM: Detection of Pneumocystis carinii with DNA amplification. Lancet 1990;336:451-453
4. Rabodonirina M, Raffenot D, Cotte L, Boibieux A, Mayençon M, Bayle G, Persat F, Rabatel F, Trepo C, Peyramond D, Piens M: Rapid detection of Pneumocystis carinii in bronchoalveolar lavage specimens from human immunodeficiency virus-infected patients: Use of a simple DNA extraction procedure and nested PCR. J Clin Microbiol 1997;35:2748-2751
5. Dahiya S, Mathur SR, Iyer VK, Kapila K, Verma K: Diagnosis of Pneumocystis pneumonia by bronchoalveolar lavage cytology: Experience at a tertiary care centre in India. Indian J Chest Dis Allied Sci 2005;47:259-265
6. Gupta R, Mirdha BR, Guleria R, Mohan A, Agarwal SK, Kumar L, Kabra SK, Samantaray JC: Improved detection of Pneumocystis jirovecii infection in a tertiary care reference hospital in India. Scand J Infect Dis 2007;39:571-576
7. Smith RL, el-Sadr WM, Lewis ML: Correlation of bronchoalveolar lavage cell populations with clinical severity of Pneumocystis carinii pneumonia. Chest 1988;93:60-64
8. Venet A, Dennewald G, Sandron D, Stern M, Jauhert F, Leibowitch J: Broncolaveolar lavage in acquired immunodeficiency syndrome (lett). Lancet 1983;2:53 (Pubitemid 13060487)
9. Tani EM, Schmitt FC, Oliveira ML, Gobetti SM, Decarls RM: Pulmonary cytology in tuberculosis. Acta Cytol 1987;31:460-463
10. Lee CH, Lu JJ, Bartlett MS, Durkin MM, Liu TH, Wang J, Jiang B, Smith JW: Nucleotide sequence variation in Pneumocystis carinii strains that infect humans. J Clin Microbiol 1993;31:754-757
11. Huang SN, Fischer SH, O'Shaughnessy E, Gill VJ, Masur H, Kovacs JA: Development of a PCR assay for diagnosis of Pneumocystis carinii pneumonia based on amplification of the multicopy major surface glycoprotein gene family. Diagn Microbiol Infect Dis 1999;35:27-32
12. Cushion MT, Beck M: Summary of Pneumocystis research presented at the 7th International Workshop on Opportunistic Protists. J Eukaryot Microbiol (suppl) 2001;48:1015-1055
13. Ognibene FP, Shelhamer J, Gill V, Macher AM, Loew D, Parker MM, Gelmann E, Fauci AS, Parrillo JE, Masur H: The diagnosis of Pneumocystis carinii pneumonia in patients with the acquired immunodeficiency syndrome using sub-segmental bronchoalveolar lavage. Am Rev Respir Dis 1984;129:929-932
14. Thomas CF, Limper AH: Pneumocystis pneumonia. N Engl J Med 2004;350:2487-2498
15. Armbruster C, Pokiesser L, Hassl A: Diagnosis of Pneumocystis pneumonia by BAL in AIDS patients: Comparison of Diff- Quik, fungi flour stain, direct IF test and PCR. Acta Cytol 1995;39:1089-1093
16. Leigh TR, Gazzard BG, Rowbottom A, Collins JV: Quantitative and qualitative comparison of DNA amplification by PCR with immunofluorescence staining for diagnosis of Pneumocystis carinii pneumonia. J Clin Pathol 1993;46:140-144
17. Cartwright CP, Nelson NA, Gill VJ: Development and evaluation of a rapid and simple procedure for detection of Pneumocystis carinii by PCR. J Clin Microbiol 1994;32:1634-1638
18. Leibovitz E, Pollack H, Moore T, Papellas J, Gallo L, Krasinski K, Borkowsky W: Comparison of PCR and standard cytological staining for detection of Pneumocystis carinii from respiratory specimens from patients with or at high risk for infection by human immunodeficiency virus. J Clin Microbiol 1995;33:3004-3007
19. Pinlaor S, Mootsikapun P, Pinlaor P, Phunmanee A, Pipitgool V, Sithithaworn P, Chumpia W, Sithithaworn J: PCR diagnosis of Pneumocystis carinii on sputum and bronchoalveolar lavage samples in immuno-compromised patients. Parasitol Res 2004;94:213-218
20. Sing A, Roggenkamp A, Autenrieth IB, Heesemann J: Pneumocystis carinii carriage in immunocompetent patients with primary pulmonary disorders as detected by single or nested PCR. J Clin Microbiol 1999;37:3409-3410
21. Mei Q, Turner RE, Sorial V, Klivington D, Angus CW, Kovacs JA: Characterization of major surface glycoprotein genes of human Pneumocystis carinii and high level expression of a conserved region. Infect Immun 1998;66:4268-4273
22. Nevez G, Raccurt C, Jounieaux V, Dei-Cas E, Mazars E: Pneumocystosis versus pulmonary Pneumocystis carinii colonization in HIV-negative and HIV-positive patients. AIDS 1999;13:535-536
23. Helweg-Larsen J, Jensen JS, Dohn B, Benfield TL, Lundgren B: Detection of Pneumocystis DNA in samples from patients suspected of bacterial pneumonia: A case-control study. BMC Infect Dis 2002;2:28
24. Maskell NA, Waine DJ, Lindley A, Pepperell JC, Wakefield AE, Miller RF, Davies RJ: Asymptomatic carriage of Pneumocystis jirovecii in subjects undergoing bronchoscopy: A prospective study. Thorax 2003;58:594-597
25. Flori P, Bellete B, Durand F, Raberin H, Cazorla C, Hafid J, Lucht F, Sung RT: Comparison between real-time PCR, conventional PCR and different staining techniques for diagnosing Pneumocystis jirovecii pneumonia from bronchoalveolar lavage specimens. J Med Microbiol 2004;53:603-607
26. Alvarez-Martínez MJ, Miró JM, Valls ME, Moreno A, Rivas PV, Solé M, Benito N, Domingo P, Muñoz C, Rivera E, Zar HJ, Wissmann G, Diehl AR, Prolla JC, de Anta MT, Gatell JM, Wilson PE, Meshnick SR, Spanish PCP Working Group: Sensitivity and specificity of nested and real-time PCR for the detection of Pneumocystis jirovecii in clinical specimens. Diagn Microbiol Infect Dis 2006;56:153-160
27. Jacobs JA, Dieleman MM, Cornelissen EI, Groen EA, Wagenaar SS, Drent M: Bronchoalveolar lavage fluid cytology in patients with Pneumocystis carinii pneumonia. Acta Cytol 2001;45:317-326
28. Sadaghdar H, Huang ZB, Eden E: Correlation of bronchoalveolar lavage findings to severity of Pneumocystis carinii pneumonia in AIDS: Evidence for the development of high-permeability pulmonary edema. Chest 1992;102:63-69