A retro-lentiviral system for doxycycline-inducible gene expression and gene knockdown in cells with limited proliferative capacity

Molecular Biology Reports

Volumn 37, Issue 4, 2010, Pages 1987-1991

  Alexeyev, Mikhail F ^a   Fayzulin, Rafik ^a   Shokolenko, Inna N ^a   Pastukh, Viktoriya ^a  

^a UNIVERSITY OF SOUTH ALABAMA   (United States)

Author keywords

Endothelial cells; Gene knockdown; Lentiviral vector; Primary cells; Regulated expression; Retroviral vector; Tet on advanced system

Indexed keywords

BETA ACTIN; DOXYCYCLINE; LENTIVIRUS VECTOR; LUCIFERASE; RETROVIRUS VECTOR; SHORT HAIRPIN RNA; ACTIN; FLUORESCENT PROTEIN 583; PHOTOPROTEIN;

ANIMAL CELL; ARTICLE; CELL PROLIFERATION; CONTROLLED STUDY; EMBRYO; ENDOTHELIUM CELL; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; HUMAN; HUMAN CELL; LUNG ENDOTHELIUM; MOLECULAR CLONING; NONHUMAN; RAT; REPORTER GENE; RNA INTERFERENCE; TRANSGENE; ANIMAL; CELL LINE; CYTOLOGY; DRUG EFFECT; FLOW CYTOMETRY; GENE SILENCING; GENE VECTOR; GENETIC TRANSDUCTION; GENETICS; LENTIVIRINAE; LUNG; METABOLISM; METHODOLOGY; MICROVASCULATURE; MOUSE; VASCULARIZATION;

LENTIVIRUS; RATTUS;

ACTINS; ANIMALS; CELL LINE; CELL PROLIFERATION; DOXYCYCLINE; ENDOTHELIAL CELLS; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; GENE KNOCKDOWN TECHNIQUES; GENETIC VECTORS; HUMANS; LENTIVIRUS; LUCIFERASES; LUMINESCENT PROTEINS; LUNG; MICE; MICROVESSELS; RATS; TRANSDUCTION, GENETIC;

EID: 77953152856     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-009-9647-7     Document Type: Article

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