Mutations in the passenger polypeptide can affect its partitioning between mitochondria and cytoplasm: Mutations can impair the mitochondrial import of DsRed

Molecular Biology Reports

Volumn 35, Issue 2, 2008, Pages 215-223

  Pastukh, Viktoriya ^a   Shokolenko, Inna N ^a   Wilson, Glenn L ^a   Alexeyev, Mikhail F ^a,b  

^a UNIVERSITY OF SOUTH ALABAMA   (United States)

^b INSTITUTE OF MOLECULAR BIOLOGY AND GENETICS   (Ukraine)

Author keywords

DsRed express; DsRed express mCherry chimera; DsRed mutant; MCherry; Mitochondrial import; Protein maturation

Indexed keywords

DNA DIRECTED DNA POLYMERASE GAMMA; POLYPEPTIDE; RED FLUORESCENT PROTEIN; SUPEROXIDE DISMUTASE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CHIMERA; CONTROLLED STUDY; CYTOPLASM; GENE MUTATION; GENE SEQUENCE; GENE SYNTHESIS; HUMAN; HUMAN CELL; MITOCHONDRION;

AMINO ACID SEQUENCE; HELA CELLS; HUMANS; LUMINESCENT PROTEINS; MITOCHONDRIA; MOLECULAR SEQUENCE DATA; MUTATION; PEPTIDES; PROTEIN SORTING SIGNALS; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; REPRODUCIBILITY OF RESULTS; SEQUENCE ALIGNMENT; SUBCELLULAR FRACTIONS;

EID: 44049093473     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-007-9073-7     Document Type: Article

References (22)

1. Taylor SW, Fahy E, Ghosh SS (2003) Global organellar proteomics. Trends Biotechnol 21(2):82-88
2. Hurt EC, Pesold-Hurt B, Schatz G (1984) The cleavable prepiece of an imported mitochondrial protein is sufficient to direct cytosolic dihydrofolate reductase into the mitochondrial matrix. FEBS Lett 178(2):306-310
3. Horwich AL et al. (1985) A leader peptide is sufficient to direct mitochondrial import of a chimeric protein. Embo J 4(5):1129-1135
4. Wilcox AJ et al. (2005) Effect of protein structure on mitochondrial import. Proc Natl Acad Sci USA 102(43):15435-15440
5. Sato T et al. (2005) Comparison of the protein-unfolding pathways between mitochondrial protein import and atomic-force microscopy measurements. Proc Natl Acad Sci USA 102(50):17999-18004
6. Matz MV et al. (1999) Fluorescent proteins from nonbioluminescent Anthozoa species. Nat Biotechnol 17(10):969-973
7. Jakobs S. et al. (2000) EFGP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy. FEBS Lett 479(3):131-135
8. Gross LA et al. (2000) The structure of the chromophore within DsRed, a red fluorescent protein from coral. Proc Natl Acad Sci USA 97(22):11990-11995
9. Baird GS, Zacharias DA, Tsien RY (2000) Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral. Proc Natl Acad Sci USA 97(22):11984-11989
10. Shaner NC et al. (2004) Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol 22(12):1567-1572
11. Bevis BJ, Glick BS (2002) Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nat Biotechnol 20(1):83-87
12. Shaner NC, Steinbach PA, Tsien RY (2005) A guide to choosing fluorescent proteins. Nat Methods 2(12):905-909
13. Alexeyev MF, Winkler HH (1999) Gene synthesis, bacterial expression and purification of the Rickettsia prowazekii ATP/ADP translocase. Biochim Biophys Acta 1419(2):299-306
14. Kozak M (1987) At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells. J Mol Biol 196(4):947-950
15. Sambrook J, Russel DW (2001) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory Press, New York
16. Ho SN et al. (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77(1):51-59
17. Smith E et al. (2005) Leaky ribosomal scanning in mammalian genomes: significance of histone H4 alternative translation in vivo. Nucleic Acids Res 33(4):1298-1308
18. Eilers M, Schatz G (1986) Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria. Nature 322(6076):228-232
19. Rassow J et al. (1989) Translocation arrest by reversible folding of a precursor protein imported into mitochondria. A means to quantitate translocation contact sites. J Cell Biol 109(4 Pt 1):1421-1428
20. Lu B et al. (2006) Tid1 isoforms are mitochondrial DnaJ-like chaperones with unique carboxyl termini that determine cytosolic fate. J Biol Chem 281(19):13150-13158
21. Karniely S, Regev-Rudzki N, Pines O (2006) The presequence of fumarase is exposed to the cytosol during import into mitochondria. J Mol Biol 358(2):396-405
22. Strobel G et al. (2002) Competition of spontaneous protein folding and mitochondrial import causes dual subcellular location of major adenylate kinase. Mol Biol Cell 13(5):1439-1448