Image analysis of two-dimensional gel electrophoresis for comparative proteomics of transgenic and non-transgenic soybean seeds

Journal of Proteomics

Volumn 73, Issue 8, 2010, Pages 1433-1440

  Brandao A R ^a   Barbosa, H S ^a   Arruda, M A Z ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

2D PAGE; Biomarkers; Genetically modified organisms; Soybean

Indexed keywords

ACTIN; ALLERGEN GLYCOPROTEIN; BETA CONGLYCININ ALPHA CHAIN PRECURSOR; BETA CONGLYCININ ALPHA SUBUNIT; GLYCININ CHAIN G2 A2B1A PRECURSOR; GLYCININ G1 PRECURSOR; SUCROSE BINDING PROTEIN; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

ARTICLE; CONTROLLED STUDY; FUNCTIONAL PROTEOMICS; HISTOGRAM; IMAGE ANALYSIS; IMAGE PROCESSING; INTERMETHOD COMPARISON; ISOELECTRIC POINT; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PLANT SEED; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; SOYBEAN; TRANSGENIC PLANT; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HYDROGEN-ION CONCENTRATION; IMAGE PROCESSING, COMPUTER-ASSISTED; PLANTS, GENETICALLY MODIFIED; PROTEOMICS; SEEDS; SOYBEAN PROTEINS; SOYBEANS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

GLYCINE MAX;

EID: 77953120293     PISSN: 18743919     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jprot.2010.01.009     Document Type: Article

References (32)

1. Wolfenbarger L.L., and Phifer P.R. The ecological risks and benefits of genetically engineered plants. Science 290 (2000) 2088-2093
2. Sinha N., and Lynch M. Fused organs in the adherent1 mutation in maize show altered epidermal walls with no perturbations in tissue identities. Planta 206 (1998) 184-195
3. Uzogara S.G. The impact of genetic modification of human foods in the 21st century: a review. Biotechnol Adv 18 (2000) 179-206
4. Kim Y., Choi S.J., Lee H., and Moon T.W. Quantitation of CP4 5-enolpyruvylshikimate-3-phosphate synthase in soybean by two-dimensional gel electrophoresis. J Microbiol Biotechnol 16 (2006) 25-31
5. Luo J., Ning T., Sun Y., Zhu J., Zhu Y., Lin Q., and Yang D. Proteomic analysis of rice endosperm cells in response to expression of hGM-CSF. J Proteome Res 8 (2009) 829-837
6. Sussulini A., Souza G.H.M.F., Eberlin M.N., and Arruda M.A.Z. Comparative metallomics for transgenic and non-transgenic soybeans. J Anal At Spectrom 22 (2007) 1501-1506
7. Görg A., Weiss W., and Dunn M.J. Current two-dimensional electrophoresis technology for proteomics. Proteomics 4 (2004) 3665-3685
8. Stessl M., Noe C.R., and Lachmann B. Influence of image-analysis software on quantitation of two-dimensional gel electrophoresis data. Electrophoresis 30 (2009) 325-328
9. Scossa F., Laudencia-Chingcuanco D., Anderson O.D., Vensel W.H., Lafiandra D., D'Ovidio R., et al. Comparative proteomic and transcriptional profiling of a bread wheat cultivar and its derived transgenic line overexpressing a low molecular weight glutenin subunit gene in the endosperm. Proteomics 8 (2008) 2948-2966
10. Chen H., Bodulovic G., Hall P.J., Moore A., Higgins T.J.V., Djordjevic M.A., et al. Unintended changes in protein expression revealed by proteomic analysis of seeds from transgenic pea expressing a bean a-amylase inhibitor gene. Proteomics 9 (2009) 4406-4415
11. Mahmood T., Jan A., and Komatsu S. Proteomic analysis of bacterial blight defence signalling pathway using transgenic rice overexpressing thaumatin-like protein. Biol Plant 53 (2009) 285-293
12. Jorrín-Novo J.V., Maldonado A.M., Echevarría-Zomeñoa S., Valledor L., Castillejo M.A., Curto M., Valero J., et al. Plant proteomics update (2007-2008): second-generation proteomic techniques, an appropriate experimental design, and data analysis to fulfill MIAPE standards, increase plant proteome coverage and expand biological knowledge. J Proteomics 72 (2009) 285-314
13. Zhu W., Venable J., Giometti C.S., Khare T., Tollaksen S., Ahrendt A.J., et al. Large-scale mu LC-MS/MS for silver- and Coomassie blue-stained polyacrylamide gels. Electrophoresis 26 (2005) 4485-4507
14. Ahsan N., Renaut J., and Komatsu S. Recent developments in the application of proteomics to the analysis of plant responses to heavy metals. Proteomics 9 (2009) 2602-2621
15. Timms J.F., and Cramer R. Difference gel electrophoresis. Proteomics 8 (2008) 4886-4897
16. Brun V., Masselon C., Garin J., and Dupuis A. Isotope dilution strategies for absolute quantitative proteomics. J Proteomics 72 (2009) 740-749
17. Moldes C.A., Médici L.O., Abrahão O.S., Tsai S.M., and Azevedo R.A. Biochemical responses of glyphosate resistant and susceptible soybean plants exposed to glyphosate. Acta Physiol Plant 30 (2008) 469-479
18. Sussulini A., Garcia J.S., Mesco M.F., Moraes D.P., Flores E.M.M., Pérez C.A., and Arruda M.A.Z. Evaluation of soybean seed protein extraction focusing on metalloprotein analysis. Microchim Acta 158 (2007) 173-180
19. Bradford M.M. Rapid and sensitive method for quantitation of microgram quantities of proteins utilizing principle of protein-dye binding. Anal Biochem 72 (1976) 248-254
20. Berkelman T., and Stenstedt T. 2-D electrophoresis-principles and methods (1998), Amersham Biosciences, Uppsala
21. Candiano G., Bruschi M., Musante L., Santucci L., Ghiggeri G.M., Carnemolla B., et al. Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis 25 (2004) 1327-1333
22. User manual, Image Master 2D Platinum 6.0, 11-0034-38, GE Healthcare, Edition AA, 2005.
23. Cutler P., Heald G., White I.R., and Ruan J. A novel approach to spot detection for two-dimensional gel electrophoresis images using pixel value collection. Proteomics 3 (2003) 392-401
24. Eravci M., Fuxius S., Broedel O., Weist S.E., Mansmann U., Schluter H., et al. Improved comparative proteome analysis based on two-dimensional gel electrophoresis. Proteomics 7 (2007) 513-523
25. Ruebelt M.C., Leimgruber N.K., Lipp M., Reynolds T.L., Nemeth M.A., Astwood J.D., et al. Application of two-dimensional gel electrophoresis to interrogate alterations in the proteome of genetically modified crops. 1. Assessing analytical validation. J Agric Food Chem 54 (2006) 2154-2161
26. Choe L.H., and Lee K.H. Quantitative and qualitative measure of intralaboratory two-dimensional protein gel reproducibility and the effects of sample preparation, sample load, and image analysis. Electrophoresis 24 (2003) 3500-3507
27. Meunier B., Bouley J., Piec I., Bernard C., Picard B., and Hocquette J.F. Data analysis methods for detection of differential protein expression in two-dimensional gel electrophoresis. Anal Biochem 340 (2005) 226-230
28. Garcia J.S., Souza G.H.M.F., Eberlin M.N., and Arruda M.A.Z. Evaluation of metal-ion stress in sunflower (Helianthus annus L.) leaves through proteomic changes. Metallomics 1 (2009) 107-113
29. Carli M.D., Villani M.E., Renzone G., Nardi L., Pasquo A., Franconi R., et al. Leaf proteome analysis of transgenic plants expressing antiviral antibodies. J Proteome Res 8 (2009) 838-848
30. Thanh V.H., and Shibasaki K. Major proteins of soybean seeds-subunit structure of beta-conglycinin. J Agric Food Chem 26 (1978) 692-695
31. Ogawa T., Samoto M., and Takahashi K. Soybean allergens and hypoallergenic soybean products. J Nutr Sci Vitaminol 46 (2000) 271-279
32. ExPASy Proteomics server, available at http://www.expasy.org.