Residues Gating the Periplasmic Pathway of LacY

Journal of Molecular Biology

Volumn 394, Issue 2, 2009, Pages 219-225

  Zhou, Yonggang ^a   Nie, Yiling ^a   Kaback, H Ronald ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

alkylation; membrane proteins; membranes; thiol cross linking; transport

Indexed keywords

ASPARAGINE; BIOTIN; CYSTEINE; ESCHERICHIA COLI PROTEIN; ISOLEUCINE; LACTOSE PERMEASE; MALEIMIDE; PROTEIN LACY; UNCLASSIFIED DRUG;

ALKYLATION; ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; CYTOPLASM; DISULFIDE BOND; GENE MUTATION; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STRUCTURE;

ESCHERICHIA COLI;

EID: 70350553821     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2009.09.043     Document Type: Article

References (42)

1. Guan L., Weinglass A.B., and Kaback H.R. Helix packing in the lactose permease of Escherichia coli: localization of helix VI. J. Mol. Biol. 312 (2001) 69-77
2. Guan L., and Kaback H.R. Lessons from lactose permease. Annu. Rev. Biophys. Biomol. Struct. 35 (2006) 67-91
3. Viitanen P., Garcia M.L., and Kaback H.R. Purified reconstituted lac carrier protein from Escherichia coli is fully functional. Proc. Natl Acad. Sci. USA 81 (1984) 1629-1633
4. Viitanen P., Newman M.J., Foster D.L., Wilson T.H., and Kaback H.R. Purification, reconstitution, and characterization of the lac permease of Escherichia coli. Methods Enzymol. 125 (1986) 429-452
5. Sahin-Tóth M., Lawrence M.C., and Kaback H.R. Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli. Proc. Natl Acad. Sci. USA 91 (1994) 5421-5425
6. Abramson J., Smirnova I., Kasho V., Verner G., Kaback H.R., and Iwata S. Structure and mechanism of the lactose permease of Escherichia coli. Science 301 (2003) 610-615
7. Mirza O., Guan L., Verner G., Iwata S., and Kaback H.R. Structural evidence for induced fit and a mechanism for sugar/H(+) symport in LacY. EMBO J. 25 (2006) 1177-1183
8. Guan L., Mirza O., Verner G., Iwata S., and Kaback H.R. Structural determination of wild-type lactose permease. Proc. Natl Acad. Sci. USA 104 (2007) 15294-15298
9. Kaback H.R., Dunten R., Frillingos S., Venkatesan P., Kwaw I., Zhang W., and Ermolova N. Site-directed alkylation and the alternating access model for LacY. Proc. Natl Acad. Sci. USA 104 (2007) 491-494
10. Majumdar D.S., Smirnova I., Kasho V., Nir E., Kong X., Weiss S., and Kaback H.R. Single-molecule FRET reveals sugar-induced conformational dynamics in LacY. Proc. Natl Acad. Sci. USA 104 (2007) 12640-12645
11. Smirnova I., Kasho V., Choe J.Y., Altenbach C., Hubbell W.L., and Kaback H.R. Sugar binding induces an outward facing conformation of LacY. Proc. Natl Acad. Sci. USA 104 (2007) 16504-16509
12. Nie Y., Ermolova N., and Kaback H.R. Site-directed alkylation of LacY: effect of the proton electrochemical gradient. J. Mol. Biol. 374 (2007) 356-364
13. Nie Y., Sabetfard F.E., and Kaback H.R. The Cys154 → Gly mutation in LacY causes constitutive opening of the hydrophilic periplasmic pathway. J. Mol. Biol. 379 (2008) 695-703
14. Zhou Y., Guan L., Freites J.A., and Kaback H.R. Opening and closing of the periplasmic gate in lactose permease. Proc. Natl Acad. Sci. USA 105 (2008) 3774-3778
15. Huang Y., Lemieux M.J., Song J., Auer M., and Wang D.N. Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli. Science 301 (2003) 616-620
16. Lemieux M.J., Song J., Kim M.J., Huang Y., Villa A., Auer M., et al. Three-dimensional crystallization of the Escherichia coli glycerol-3-phosphate transporter: a member of the major facilitator superfamily. Protein Sci. 12 (2003) 2748-2756
17. Law C.J., Yang Q., Soudant C., Maloney P.C., and Wang D.N. Kinetic evidence is consistent with the rocker-switch mechanism of membrane transport by GlpT. Biochemistry 46 (2007) 12190-12197
18. Dawson R.J., and Locher K.P. Structure of a bacterial multidrug ABC transporter. Nature 443 (2006) 180-185
19. Khare D., Oldham M.L., Orelle C., Davidson A.L., and Chen J. Alternating access in maltose transporter mediated by rigid-body rotations. Mol. Cell 33 (2009) 528-536
20. Bogdanov M., Heacock P.N., and Dowhan W. A polytopic membrane protein displays a reversible topology dependent on membrane lipid composition. EMBO J. 21 (2002) 2107-2116
21. le Coutre J., Narasimhan L.R., Patel C.K., and Kaback H.R. The lipid bilayer determines helical tilt angle and function in lactose permease of Escherichia coli. Proc. Natl Acad. Sci. USA 94 (1997) 10167-10171
22. le Coutre J., Kaback H.R., Patel C.K., Heginbotham L., and Miller C. Fourier transform infrared spectroscopy reveals a rigid alpha-helical assembly for the tetrameric Streptomyces lividans K+ channel. Proc. Natl Acad. Sci. USA 95 (1998) 6114-6117
23. Nie Y., Smirnova I., Kasho V., and Kaback H.R. Energetics of ligand-induced conformational flexibility in the lactose permease of Escherichia coli. J. Biol. Chem. 281 (2006) 35779-35784
24. Jensen M.O., Yin Y., Tajkhorshid E., and Schulten K. Sugar transport across lactose permease probed by steered molecular dynamics. Biophys. J. 93 (2007) 92-102
25. Pelis R.M., Zhang X., Dangprapai Y., and Wright S.H. Cysteine accessibility in the hydrophilic cleft of human organic cation transporter 2. J. Biol. Chem. 281 (2006) 35272-35280
26. Karlin A., and Akabas M.H. Substituted-cysteine accessibility method. Methods Enzymol. 293 (1998) 123-145
27. Kwaw I., Zen K.C., Hu Y., and Kaback H.R. Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: helices IV and V that contain the major determinants for substrate binding. Biochemistry 40 (2001) 10491-10499
28. Nie Y., Zhou Y., and Kaback H.R. Clogging the periplasmic pathway in LacY. Biochemistry 48 (2009) 738-743
29. Guan L., and Kaback H.R. Binding affinity of lactose permease is not altered by the H+ electrochemical gradient. Proc. Natl Acad. Sci. USA 101 (2004) 12148-12152
30. Sahin-Tóth M., Gunawan P., Lawrence M.C., Toyokuni T., and Kaback H.R. Binding of hydrophobic d-galactopyranosides to the lactose permease of Escherichia coli. Biochemistry 41 (2002) 13039-13045
31. Frillingos S., and Kaback H.R. Cysteine-scanning mutagenesis of helix VI and the flanking hydrophilic domains in the lactose permease of Escherichia coli. Biochemistry 35 (1996) 5333-5338
32. Wolin C.D., and Kaback H.R. Thiol cross-linking of transmembrane domains IV and V in the lactose permease of Escherichia coli. Biochemistry 39 (2000) 6130-6135
33. Law C.J., Almqvist J., Bernstein A., Goetz R.M., Huang Y., Soudant C., et al. Salt-bridge dynamics control substrate-induced conformational change in the membrane transporter GlpT. J. Mol. Biol. 378 (2008) 826-837
34. Widdas W.F. Inability of diffusion to account for placental glucose transfer in the sheep and consideration of the kinetics of a possible carrier transfer. J. Physiol. 118 (1952) 23-39
35. Mitchell P. Molecule, group and electron transport through natural membranes. Biochem. Soc. Symp. 22 (1963) 142-168
36. Jardetzky O. Simple allosteric model for membrane pumps. Nature 211 (1966) 969-970
37. Akabas M.H., Stauffer D.A., Xu M., and Karlin A. Acetylcholine receptor channel structure probed in cysteine-substitution mutants. Science 258 (1992) 307-310
38. Carrasco N., Viitanen P., Herzlinger D., and Kaback H.R. Monoclonal antibodies against the lac carrier protein from Escherichia coli. 1. Functional studies. Biochemistry 23 (1984) 3681-3687
39. Teather R.M., Müller-Hill B., Abrutsch U., Aichele G., and Overath P. Amplification of the lactose carrier protein in Escherichia coli using a plasmid vector. Mol. Gen. Genet. 159 (1978) 239-248
40. Kaback H.R. Bacterial membranes. In: Kaplan N.P., Jakoby W.B., and Colowick N.P. (Eds). Methods in Enzymology XXII (1971), Elsevier, New York, NY 99-120
41. Short S.A., Kaback H.R., and Kohn L.D. Localization of d-lactate dehydrogenase in native and reconstituted Escherichia coli membrane vesicles. J. Biol. Chem. 250 (1975) 4291-4296
42. Carrasco N., Herzlinger D., Mitchell R., DeChiara S., Danho W., Gabriel T.F., and Kaback H.R. Intramolecular dislocation of the COOH terminus of the lac carrier protein in reconstituted proteoliposomes. Proc. Natl Acad. Sci. USA 81 (1984) 4672-4676