Mutations on aromatic residues of the active site to alter selectivity of the Sulfolobus solfataricus maltooligosyltrehalose synthase

Journal of Agricultural and Food Chemistry

Volumn 54, Issue 10, 2006, Pages 3585-3590

  Fang, Tsuei Yun ^a   Tseng, Wen Chi ^b   Chung, Yao Te ^a   Pan, Ching Hsing ^a  

^a NATIONAL TAIWAN OCEAN UNIVERSITY   (Taiwan)

^b NATIONAL TAIWAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Taiwan)

Author keywords

Maltooligosyltrehalose synthase; Selectivity; Site directed mutagenesis; Substrate specificity; Sulfolobus; Trehalose

Indexed keywords

GLUCOSYLTRANSFERASE; MALTOOLIGOSYL TREHALOSE SYNTHASE; TREHALOSE;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; GLYCOSYLATION; HYDROLYSIS; METABOLISM; MUTATION; SULFOLOBUS SOLFATARICUS;

AMINO ACID SEQUENCE; BINDING SITES; CATALYTIC DOMAIN; GLUCOSYLTRANSFERASES; GLYCOSYLATION; HYDROLYSIS; MUTATION; SUBSTRATE SPECIFICITY; SULFOLOBUS SOLFATARICUS; TREHALOSE;

SULFOLOBUS; SULFOLOBUS SOLFATARICUS;

EID: 33744924721     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf060152z     Document Type: Article

References (29)

1. Richards, A. B.; Krakowka, S.; Dexter, L. B.; Schmid, H.; Wolterbeek, A. P.; Waalkens-Berendsen, D. H.; Shigoyuki, A.; Kurimoto, M. Trehalose: a review of properties, history of use and human tolerance, and results of multiple safety studies. Food Chem. Toxicol. 2002, 40, 871-898.
2. Kato, M.; Miura, Y.; Kettoku, M.; Shindo, K.; Iwamatsu, A.; Kobayashi, K. Purification and characterization of new trehalose-producing enzymes isolated from the hyperthermophilic archae, Sulfolobus solfataricus KM1. Biosci., Biotechnol., Biochem. 1996, 60, 546-550.
3. Nakada, T.; Ikegami, S.; Chaen, H.; Kubota, M.; Fukuda, S.; Sugimoto, T.; Kurimoto, M.; Tsujisaka, Y. Purification and characterization of thermostable maltooligosyl trehalose synthase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius. Biosci., Biotechnol., Biochem. 1996, 60, 263-266.
4. Nakada, T.; Ikegami, S.; Chaen, H.; Kubota, M.; Fukuda, S.; Sugimoto, T.; Kurimoto, M.; Tsujisaka, Y. Purification and characterization of thermostable maltooligosyl trehalose trehalohydrolase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius. Biosci., Biotechnol., Biochem. 1996, 60, 267-270.
5. Kato, M. Trehalose production with a new enzymatic system from Sulfolobus solfataricus KM1. J. Mol. Catal. B 1999, 6, 223-233.
6. Kobayashi, K.; Komeda, T.; Miura, Y.; Kettoku, M.; Kato, M. Production of trehalose from starch by novel trehalose-producing enzymes from Sulfolobus solfataricus KM1. J. Ferment. Bioeng. 1997, 83, 296-298.
7. Mukai, K.; Tabuchi, A.; Nakada, T.; Shibuya, T.; Chaen, H.; Fukuda, S.; Kurimoto, M.; Tsujisaka, Y. Production of trehalose from starch by thermostable enzymes from Sulfolobus acidocaldarius. Starch/Staerke 1997, 49, 26-30.
8. Fang, T. Y.; Coutinho, P. M.; Reilly, P. J.; Ford, C. Mutations to alter Aspergillus awamori glucoamylase selectivity. I. Tyr48Phe49→Trp, Tyr116→Trp, Tyr175→Phe, Arg241→Lys, Ser411→Ala and Ser411-Gly. Protein Eng. 1998, 11, 119-126.
9. Fang, T. Y.; Honzatko, R. B.; Reilly, P. J.; Ford, C. Mutations to alter Aspergillus awamori glucoamylase selectivity. II. Mutation of residues 119 and 121. Protein Eng. 1998, 11, 127-133.
10. Ford, C. Improving operating performance of glucoamylase by mutagenesis. Curr. Opin. Biotechnol. 1999, 10, 353-357.
11. Fersht, A. R.; Shi, J. P.; Knill-Jones, J.; Lowe, D. M.; Wilkinson, A. J.; Blow, D. M.; Brick, P.; Carter, P.; Waye, M. M.; Winter, G. Hydrogen bonding and biological specificity analysed by protein engineering. Nature 1985, 314, 235-238.
12. Kato, M.; Takehara, K.; Kettoku, M.; Kobayashi, K.; Shimizu, T. Subsite structure and catalytic mechanism of a new glycosyltrehalose-producing enzyme isolated from the hyperthermophilic archaeum, Sulfolobus solfataricus KM1. Biosci., Biotechnol., Biochem. 2000, 64, 319-326.
13. Kobayashi, M.; Kubota, M.; Matsuura, Y. Refined structure and functional implications of trehalose synthase from Sulfolobus acidocaldarius. J. Appl. Glycosci. 2003, 50, 1-8.
14. MacGregor, E. A.; Janecek, S.; Svensson, B. Relationship of sequence and structure to specificity in the alpha-amylase family of enzymes. Biochim. Biophys. Acta 2001, 1546, 1-20.
15. Fang, T. Y.; Hung, X. G.; Shih, T. Y.; Tseng, W. C. Characterization of the trehalosyl dextrin-forming enzyme from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092. Extremophiles 2004, 8, 335-343.
16. Nelson, M.; McClelland, M. Use of DNA methyltransferase/endonuclease enzyme combinations for megabase mapping of chromosomes. Methods Enzymol. 1992, 216, 279-303.
17. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
18. Miller, G. L. Use of dinitrosalicilic acid reagent for determination of reducing sugar. Anal. Chem. 1959, 31, 426-428.
19. Rabbo, E.; Terkildsen, T. C. Enzymic determination of blood glucose. Scand. J. Clin. Lab. Invest. 1960, 12, 402-407.
20. Wilkinson, A. J.; Fersht, A. R.; Blow, D. M.; Winter, G. Site-directed mutagenesis as a probe of enzyme structure and catalysis: tyrosyl-tRNA synthetase cysteine-35 to glycine-35 mutation. Biochemistry 1983, 22, 3581-3586.
21. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254.
22. Morley, K. L.; Kazlauskas, R. J. Improving enzyme properties: when are closer mutations better? Trends Biotechnol. 2005, 23, 231-237.
23. Erlandsen, H.; Bjorgo, E.; Flatmark, T.; Stevens, R. C. Crystal structure and site-specific mutagenesis of pterin-bound human phenylalanine hydroxylase. Biochemistry 2000, 39, 2208-2217.
24. Fierke, C. A.; Calderone, T. L.; Krebs, J. F. Functional consequences of engineering the hydrophobic pocket of carbonic anhydrase II. Biochemistry 1991, 30, 11054-11063.
25. Suzukawa, K.; Yamagami, T.; Ohnuma, T.; Hirakawa, H.; Kuhara, S.; Aso, Y.; Ishiguro, M. Mutational analysis of amino acid residues involved in catalytic activity of a family 18 chitinase from tulip bulbs. Biosci., Biotechnol., Biochem. 2003, 67, 341-346.
26. Li, C. H.; Tu, S. C. Active site hydrophobicity is critical to the bioluminescence activity of Vibrio harveyi luciferase. Biochemistry 2005, 44, 12970-12977.
27. Siddiqi, F.; Bourque, J. R.; Jiang, H.; Gardner, M.; St Maurice, M.; Blouin, C.; Bearne, S. L. Perturbing the hydrophobic pocket of mandelate racemase to probe phenyl motion during catalysis. Biochemistry 2005, 44, 9013-9021.
28. Davies, G. J.; Wilson, K. S.; Henrissat, B. Nomenclature for sugar-binding subsites in glycosyl hydrolases. Biochem. J. 1997, 321, 557-559.
29. de Pascale, D.; Sasso, M. P.; Lernia, I. D.; Lazzaro, A. D.; Furia, A.; Farina, M. C.; Rossi, M.; De Rosa, M. Recombinant thermophilic enzymes for trehalose and trehalosyl dextrins production. J. Mol. Catal. B 2001, 11, 777-786.