Cloning, expression, and characterization of a thermostable glucoamylase from Thermoanaerobacter tengcongensis MB4

Applied Microbiology and Biotechnology

Volumn 87, Issue 1, 2010, Pages 225-233

  Zheng, Yingying ^a,b   Xue, Yanfen ^a   Zhang, Yueling ^c   Zhou, Cheng ^a   Schwaneberg, Ulrich ^d   Ma, Yanhe ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^d RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

Glucoamylase; Recombinant expression; Thermoanaerobacter tengcongensis; Thermostable

Indexed keywords

AMINO ACIDS; CALCIUM; CLONING; ENZYMES; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GELS; GENE ENCODING; GLUCOSE; LEAD; MANGANESE; MANGANESE COMPOUNDS; ORGANIC ACIDS; POLYSACCHARIDES; ZINC;

ACTIVITY LOSS; GEL-FILTRATION CHROMATOGRAPHY; GLUCANS; GLUCOAMYLASE; GLYCOSIDIC LINKAGES; KINETIC STUDY; MALTOOLIGOSACCHARIDES; MATURE PROTEINS; RECOMBINANT ENZYMES; RECOMBINANT EXPRESSION; SIGNAL PEPTIDE; SPECIFIC ACTIVITY;

ENZYME ACTIVITY;

CALCIUM; COBALT; GLUCAN 1,4 ALPHA GLUCOSIDASE; MAGNESIUM; MALTOSE; MANGANESE; RECOMBINANT ENZYME; BACTERIAL PROTEIN; RECOMBINANT PROTEIN;

AMINO ACID; CATION; CLONE; COLIFORM BACTERIUM; DDT; ENZYME ACTIVITY; GENE EXPRESSION; HOMOGENEITY; INCUBATION; PEPTIDE; POLYSACCHARIDE; RECOMBINATION; THERMOPHILIC BACTERIUM;

ARTICLE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; GENE EXPRESSION; HEAT TREATMENT; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PROTEIN EXPRESSION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; THERMOANAEROBACTER; THERMOANAEROBACTER TENGCONGENSIS; THERMOSTABILITY; AMINO ACID SEQUENCE; CHEMISTRY; CONFORMATION; ENZYME STABILITY; ENZYMOLOGY; GENETICS; HEAT; KINETICS; METABOLISM; MOLECULAR GENETICS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; THERMOANAEROBACTER TENGCONGENSIS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CLONING, MOLECULAR; ENZYME STABILITY; GENE EXPRESSION; GLUCAN 1,4-ALPHA-GLUCOSIDASE; HOT TEMPERATURE; KINETICS; MOLECULAR CONFORMATION; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; THERMOANAEROBACTER;

EID: 77952747139     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2439-0     Document Type: Article

References (34)

1. AE Aleshin A Golubev LM Firsov RB Honzatko 1992 Crystal structure of glucoamylase from Aspergillus awamori var. X100 to 2.2-A resolution J Biol Chem 267 27 19291 19298 1:CAS:528:DyaK38XmtVOrtbw%3D
2. AE Aleshin PH Feng RB Honzatko PJ Reilly 2003 Crystal structure and evolution of a prokaryotic glucoamylase J Mol Biol 327 61 73 10.1016/S0022-2836(03)00084-6 1:CAS:528:DC%2BD3sXhsVCgurk%3D (Pubitemid 36293302)
3. SF Altschul W Gish W Miller EW Myers DJ Lipman 1990 Basic local alignment search tool J Mol Biol 215 403 410 1:CAS:528:DyaK3MXitVGmsA%3D%3D
4. ACMM Aquino JA Jorge HF Terenzi MLT Polizeli 2001 Thermostable glucose-tolerant glucoamylase produced by the thermophilic fungus Scytalidium thermophilum Folia Microbiol 46 1 11 16 10.1007/BF02825876 1:CAS:528: DC%2BD3MXjs1WhsLc%3D
5. K Arnold L Bordoli J Kopp T Schwede 2006 The SWISSMODEL workspace: a web-based environment for protein structure homology modeling Bioinformatics 22 195 201 10.1093/bioinformatics/bti770 1:CAS:528:DC%2BD28XovVCltw%3D%3D (Pubitemid 43205406)
6. Q Bao Y Tian W Li Z Xu Z Xuan S Hu W Dong J Yang Y Chen Y Xue Y Xu X Lai L Huang X Dong Y Ma L Ling H Tan R Chen J Wang J Yu H Yang 2002 A complete sequence of the T. tengcongensis genome Genome Res 12 689 700 10.1101/gr.219302 1:CAS:528:DC%2BD38XjvFSgt7g%3D (Pubitemid 34546457)
7. H Bender 1981 A bacterial glucoamylase degrading cyclodextrins. Partial purification and properties of the enzyme from a Flavobacteikn species Eur J Biochem 115 287 291 10.1111/j.1432-1033.1981.tb05236.x 1:CAS:528: DyaL3MXhvVOmsb8%3D (Pubitemid 11103631)
8. JD Bendtsen H Nielsen G Heijne S Brunak 2004 Improved prediction of signal peptides: SignalP 3.0 J Mol Biol 340 783 795 10.1016/j.jmb.2004.05.028 (Pubitemid 38829638)
9. PM Coutinho PJ Reilly 1994 Structure-function relationships in the catalytic and starch binding domains of glucoamylase Protein Eng 7 3 393 400 10.1093/protein/7.3.393 1:CAS:528:DyaK2cXjtFyhsLY%3D
10. C Dock M Hess G Antranikian 2008 A thermoactive glucoamylase with biotechnological relevance from the thermoacidophilic euryarchaeon Thermoplasma acidophilum Appl Microbiol Biotechnol 78 105 114 10.1007/s00253-007-1293-1 1:CAS:528:DC%2BD1cXot1KksQ%3D%3D
11. A Ducki O Grundmann L Konermann F Mayer M Hoppert 1998 Glucoamylase from Thermoanaerobacterium thermosaccharolyticum: sequence studies and analysis of the macromolecular architecture of the enzyme J Gen Appl Microbiol 44 327 335 10.2323/jgam.44.327 1:CAS:528:DyaK1MXhsFWntrY%3D (Pubitemid 29060423)
12. T Fagerstrom N Kalkkinen 1995 Characterization, subsite mapping and partial amino acid sequence of glucoamylase from the filamentous fungus Trichoderma reesei Biotechnol Appl Bioc 21 223 231
13. P Feng S Berensmeier K Buchholz PJ Reilly 2002 Production, purification, and characterization of Thermoanaerobacterium thermosaccharolyticum glucoamylase Starch 54 328 337 10.1002/1521-379X(200208)54:8<328::AID-STAR328>3.0. CO;2-7 1:CAS:528:DC%2BD38XmslSru7c%3D (Pubitemid 34963618)
14. WM Fogarty CP Benson 1983 Purification and properties of a thermophilic amyloglucosidase from Aspergillus niger Eur J Appl Microbiol Biotechnol 19 271 278 10.1007/BF00500491
15. HH Hyun JG Zeikus 1985 General biochemical characterization of thermostable pullulanase and glucoamylase from Clostridium thermohydrosulfuricum Appl Environ Microbiol 49 5 1168 1173 1:CAS:528:DyaL2MXktValtLo%3D
16. JA James JL Berger BH Lee 1997 Purification of glucoamylase from Lactobacillus amylovorus ATCC 33621 Curr Microbiol 34 186 191 10.1007/s002849900166 1:CAS:528:DyaK2sXhsVKlur4%3D (Pubitemid 27085951)
17. MS Kim JT Park YW Kim HS Lee R Nyawira HS Shin CS Park SH Yoo YR Kim TW Moon KH Park 2004 Properties of a novel thermostable glucoamylase from the hyperthermophilic archaeon Sulfolobus solfataricus in relation to starch processing Appl Environ Microbiol 70 7 3933 3940 10.1128/AEM.70.7.3933-3940.2004 1:CAS:528:DC%2BD2cXmtFWntr8%3D (Pubitemid 38969731)
18. RA Laskowski MW MacArthur DS Moss JM Thornton 1993 PROCHECK: a program to check the stereochemical quality of protein structures J App Cryst 26 283 291 10.1107/S0021889892009944 1:CAS:528:DyaK3sXit12lurY%3D
19. Y Li PM Coutinho C Ford 1998 Effect on thermostability and catalytic activity of introducing disulfide bonds into Aspergillus awamori glucoamylase Protein Eng 11 661 667 10.1093/protein/11.8.661 1:CAS:528:DyaK1cXmtVWqurk%3D
20. H Liu W Wang 2003 Protein engineering to improve the thermostability of glucoamylase from Aspergillus awamori based on molecular dynamics simulations Protein Eng 16 19 25 10.1093/proeng/gzg007 (Pubitemid 36407061)
21. H Liu Y Doleyres PM Coutinho C Ford PJ Reilly 2000 Replacement and deletion mutations in the catalytic domain and belt region of Aspergillus awamori glucoamylase to enhance thermostability Protein Eng 13 655 659 10.1093/protein/13.9.655 1:CAS:528:DC%2BD3cXosVSrtb0%3D
22. AL Morris MW MacArthur EG Hutchinson JM Thornton 1992 Stereochemical quality of protein structure coordinates Proteins 12 345 364 10.1002/prot.340120407 1:CAS:528:DyaK38XisFWls7o%3D
23. H Nielsen J Engelbrecht J Brunak G Heijne 1997 Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites Protein Eng 10 1 6 10.1093/protein/10.1.1 1:CAS:528:DyaK2sXhsVersrs%3D
24. H Ohnishi H Sakai T Ohta 1991 Purification and some properties of a glucoamylase from Clostridium sp. G0005 Agric Biol Chem 55 7 1901 1902 1:CAS:528:DyaK3MXltlSntbw%3D
25. H Ohnishi H Kitamura T Minowa H Sakai T Ohta 1992 Molecular cloning of a glucoamylase gene from a thermophilic Clostridium and kinetics of the cloned enzyme Eur J Biochem 207 413 418 10.1111/j.1432-1033.1992.tb17064.x 1:CAS:528:DyaK3sXkvV2rtrY%3D
26. A Oren 1983 A thermophilic amyloglucosidase from Halobacterium sodomense, a halophilic bacterium from the Dead Sea Curr Microbiol 8 225 230 10.1007/BF01579551 1:CAS:528:DyaL3sXktF2gsbY%3D (Pubitemid 13063352)
27. E Serour G Antranikian 2002 Novel thermoactive glucoamylases from the thermoacidophilic Archaea Thermoplasma acidophilum, Picrophilus torridus and Picrophilus oshimae Anton Leeuw Int J G 81 73 83 10.1023/A:1020525525490 1:CAS:528:DC%2BD38Xns1Sgs7g%3D (Pubitemid 35314598)
28. U Specka F Mayer G Antranikian 1991 Purification and properties of a thermoactive glucoamylase from Clostridium thermosaccharolyticum Appl Environ Microbiol 57 8 2317 2323 1:CAS:528:DyaK3MXlsFels7s%3D
29. LBB Spinelli ML Polizeli HF Terenzi JA Jorge 1996 Biochemical characterization of glucoamylase from the hyperproducer exo-1 mutant strain of Neurospora crassa FEMS Microbiol Lett 138 173 177 10.1111/j.1574-6968.1996. tb08152.x
30. RAK Srivastava 1984 Studies on extracellular and intracellular purified amylases from a thermophilic Bacillus stearothermophilus Enzyme Microb Tech 6 9 422 426 10.1016/0141-0229(84)90018-8 1:CAS:528:DyaL2cXmt1GntLs%3D (Pubitemid 14643424)
31. FW Studier 2005 Protein production by auto-induction in high-density shaking cultures Protein Expres Purif 41 207 234 10.1016/j.pep.2005.01.016 1:CAS:528:DC%2BD2MXjsFCktLw%3D
32. JD Thompson TJ Gibson F Plewniak F Jeanmougin DG Higgins 1997 The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools Nucleic Acids Res 25 4876 4882 10.1093/nar/25.24.4876 1:CAS:528:DyaK1cXntFyntQ%3D%3D (Pubitemid 28022245)
33. R Uotsu-Tomita T Tonozuka H Sakai Y Sakano 2001 Novel glucoamylase-type enzymes from Thermoactinomyces vulgaris and Methanococcus jannaschii whose genes are found in the flanking region of the α-amylase genes Appl Microbiol Biotechnol 56 465 473 10.1007/s002530100609 1:CAS:528:DC%2BD3MXmsFKhu7c%3D (Pubitemid 32763364)
34. Y Xue Y Xu Y Liu Y Ma P Zhou 2001 Thermoanaerobacter tengcongensis sp. nov., a novel anaerobic, saccharolytic, thermophilic bacterium isolated from a hot spring in Tengcong, China Int J Syst Evol Micr 51 1335 1341 1:CAS:528:DC%2BD3MXmtVSmurs%3D