A fluorescence correlation spectroscopy-based assay for fragment screening of slowly inhibiting protein-peptide interaction inhibitors

Analytical Biochemistry

Volumn 402, Issue 1, 2010, Pages 26-31

  Mikuni, Junko ^a   Kato, Miki ^a   Taruya, Shigenao ^a   Tsuganezawa, Keiko ^a   Mori, Masumi ^a   Ogawa, Naoko ^a   Honma, Teruki ^a   Yokoyama, Shigeyuki ^a,b   Kojima, Hirotatsu ^b   Okabe, Takayoshi ^b   Nagano, Tetsuo ^b   Tanaka, Akiko ^a,b  

^a RIKEN   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

FBDD; FCS; Fragment screening; Protein protein interaction inhibitor

Indexed keywords

FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; SPECTROSCOPIC ANALYSIS; SURFACE PLASMON RESONANCE;

COMPETITIVE BINDING ASSAY; DIRECT BINDINGS; FBDD; FLUORESCENCE CORRELATION SPECTROSCOPY; INTERACTION SITE; PHOSPHOPEPTIDES; PROTEIN-PEPTIDE INTERACTIONS; PROTEIN-PROTEIN INTERACTIONS;

PEPTIDES;

BIOTIN; CBL PROTEIN; EPIDERMAL GROWTH FACTOR RECEPTOR; PHOSPHOPEPTIDE; PROTEIN APS; PROTEIN KINASE ZAP 70; UNCLASSIFIED DRUG;

ARTICLE; COMPETITIVE INHIBITION; CONTROLLED STUDY; DIFFUSION; FLUORESCENCE CORRELATION SPECTROSCOPY; MOLECULAR PROBE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; SURFACE PLASMON RESONANCE;

EID: 77952744088     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.03.019     Document Type: Article

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