A quantitative mass spectrometry-based approach for identifying protein kinase clients and quantifying kinase activity

Analytical Biochemistry

Volumn 402, Issue 1, 2010, Pages 69-76

  Huang, Yadong ^a   Houston, Norma L ^a   Tovar Mendez, Alejandro ^a   Stevenson, Severin E ^a   Miernyk, Jan A ^a,b   Randall, Douglas D ^a   Thelen, Jay J ^a  

^a University of Missouri   (United States)

^b U S DEPARTMENT OF AGRICULTURE   (United States)

Author keywords

CPK3; Mass spectrometry; Method; PDK; Peptide kinase assay; Protein phosphorylation

Indexed keywords

MASS SPECTROMETRY; PEPTIDES; PHOSPHORYLATION; PLANTS (BOTANY);

CALCIUM DEPENDENT PROTEIN KINASE; CPK3; KINASE ASSAYS; METHOD; PEPTIDE PHOSPHORYLATION; PROTEIN PHOSPHORYLATION; PYRUVATE DEHYDROGENASE KINASE; QUANTITATIVE MASS SPECTROMETRIES;

ENZYMES;

CALCIUM DEPENDENT PROTEIN KINASE 3; METHIONINE; PROTEIN KINASE; PYRUVATE DEHYDROGENASE KINASE; SYNTHETIC PEPTIDE; TYROSYLHISTIDINYLGLYCINYLHISTIDINYLSERYLMETHIONYLSERYLASPARTYLPROLYLGLYCINYLSERYLTHREONYLTYROSYLARGININE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SUBSTRATE; ENZYME SUBUNIT; IN VITRO STUDY; MASS SPECTROMETRY; OXIDATION; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SCREENING TEST; SENSITIVITY AND SPECIFICITY; VALIDATION STUDY;

ARABIDOPSIS THALIANA; HOMO SAPIENS;

EID: 77952742019     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.03.028     Document Type: Article

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