Laser excitation power and the flow cytometric resolution of complex karyotypes

Cytometry Part A

Volumn 77, Issue 6, 2010, Pages 585-588

  Ng, Bee L ^a   Carter, Nigel P ^a  

^a WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

Author keywords

Chromosomes; Flow karyotype; Laser power; Photo saturation; Resolution

Indexed keywords

ARTICLE; CELL CULTURE; FLOW CYTOMETRY; HUMAN; KARYOTYPING; LASER; METHODOLOGY; TUMOR CELL LINE;

CELL LINE, TUMOR; CELLS, CULTURED; FLOW CYTOMETRY; HUMANS; KARYOTYPING; LASERS;

EID: 77952722695     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20904     Document Type: Article

References (12)

1. Marx J. Medicine. Fused genes may help explain the origins of prostate cancer. Science 2005;310:603.
2. Tomlins SA, Rhodes DR, Perner S, Dhanasekaran SM, Mehra R, Sun XW, Varambally S, Cao X, Tchinda J, Kuefer R, et al. Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer. Science 2005;310:644-648.
3. Yin CC, Cortes J, Barkoh B, Hayes K, Kantarjian H, Jones D. t(3;21)(q26;q22) in myeloid leukemia: An aggressive syndrome of blast transformation associated with hydroxyurea or antimetabolite therapy. Cancer 2006;106:1730-1738.
4. Gasparini P, Sozzi G, Pierotti MA. The role of chromosomal alterations in human cancer development. J Cell Biochem 2007;102:320-331.
5. Burrow AA, Williams LE, Pierce LC, Wang YH. Over half of breakpoints in gene pairs involved in cancer-specific recurrent translocations are mapped to human chromosomal fragile sites. BMC Genomics 2009;10:59.
6. Gray JW, Trask B, van den Engh G, Silva A, Lozes C, Grell S, Schonberg S, Yu LC, Golbus MS. Application of flow karyotyping in prenatal detection of chromosome aberrations. Am J Hum Genet 1988;42:49-59.
7. Lebo RV, Golbus MS, Cheung MC. Detecting abnormal human chromosome constitutions by dual laser flow cytogenetics. Am J Med Genet 1986;25:519-529.
8. Cram LS, Bartholdi MF, Ray FA, Travis GL, Jett JH, Kraemer PM. Quantitation of one aspect of karyotype instability associated with neoplastic transformation in Chinese hamster cells. Prog Nucleic Acid Res Mol Biol 1983;29:39-42.
9. Howarth KD, Blood KA, Ng BL, Beavis JC, Chua Y, Cooke SL, Raby S, Ichimura K, Collins VP, Carter NP, et al. Array painting reveals a high frequency of balanced translocations in breast cancer cell lines that break in cancer-relevant genes. Oncogene 2008;27:3345-3359.
10. Frey T, Houck DW, Shenker BJ, Hoffman RA. Bivariate flow karyotyping with aircooled lasers. Cytometry 1994;16:169-174.
11. Ng BL, Carter NP. Factors affecting flow karyotype resolution. Cytometry A 2006;69A:1028-1036.
12. Bartholdi MF, Sinclair DC, Cram LS. Chromosome analysis by high illumination flow cytometry. Cytometry 1983;3:395-401.