NMR-based metabonomic investigation of heat stress in myotubes reveals a time-dependent change in the metabolites

Journal of Agricultural and Food Chemistry

Volumn 58, Issue 10, 2010, Pages 6376-6386

  Straadt, Ida K ^a   Young, Jette F ^a   Bross, Peter ^b   Gregersen, Niels ^b   Oksbjerg, Niels ^a   Theil, Peter K ^a   Bertram, Hanne C ^b  

^a DANISH INSTITUTE OF AGRICULTURAL SCIENCES   (Denmark)

^b AARHUS UNIVERSITY   (Denmark)

Author keywords

Acetate; Heat shock protein; Hsp70; Lactate; Metabolites; Metabonomics; Multivariate data analysis; Muscle cells; Overshoot; Proton nuclear magnetic resonance; Slaughter stress

Indexed keywords

AMINO ACID; GLUCOSE; HEAT SHOCK PROTEIN 70; LACTIC ACID; MESSENGER RNA;

ANIMAL; ARTICLE; CELL CULTURE; CELL LINE; CHEMISTRY; GENETICS; HEAT; METABOLISM; METABOLOMICS; MOUSE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PH; SKELETAL MUSCLE; SWINE; TIME;

AMINO ACIDS; ANIMALS; CELL LINE; CELLS, CULTURED; GLUCOSE; HOT TEMPERATURE; HSP70 HEAT-SHOCK PROTEINS; HYDROGEN-ION CONCENTRATION; LACTIC ACID; MAGNETIC RESONANCE SPECTROSCOPY; METABOLOMICS; MICE; MUSCLE FIBERS, SKELETAL; RNA, MESSENGER; SWINE; TIME FACTORS;

SUIDAE;

EID: 77952653783     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf904197u     Document Type: Article

References (32)

1. van der Wal, P. G.; Engel, B.; Reimert, H. G. M. The effect of stress, applied immediately before stunning, on pork quality Meat Sci. 1999, 53, 101-106
2. Stoier, S.; Aaslyng, M. D.; Olsen, E. V.; Henckel, P. The effect of stress during lairage and stunning on muscle metabolism and drip loss in Danish pork Meat Sci. 2001, 59, 127-131
3. Alvarez, D.; Garrido, M. D.; Banon, S. Influence of Pre-Slaughter Process on Pork Quality: An Overview Food Res. Int. 2009, 25, 233-250
4. Srinongkote, S.; Smriga, M.; Nakagawa, K.; Toride, Y. A diet fortified with L-lysine and L-arginine reduces plasma cortisol and blocks anxiogenic response to transportation in pigs Nutr. Neurosci. 2003, 6, 283-289
5. Troeger, K.; Woltersdorf, W. Measuring Stress in Pigs During Slaughter Fleischwirtschaft 1989, 69, 373-376
6. DSouza, D. N.; Dunshea, F. R.; Warner, R. D.; Leury, B. J. The effect of handling pre-slaughter and carcass processing rate post-slaughter on pork quality Meat Sci. 1998, 50, 429-437
7. Bertram, H. C.; Stodkilde-Jorgensen, H.; Karlsson, A. H.; Andersen, H. J. Post mortem energy metabolism and meat quality of porcine M-longissimus dorsi as influenced by stunning method-A P-31 NMR spectroscopic study Meat Sci. 2002, 62, 113-119
8. Shen, Q. W.; Means, W. J.; Thompson, S. A.; Underwood, K. R.; Zhu, M. J.; McCormick, R. J.; Ford, S. P.; Du, M. Pre-slaughter transport, AMP-activated protein kinase, glycolysis, and quality of pork loin Meat Sci. 2006, 74, 388-395
9. Young, J. F.; Bertram, H. C.; Oksbjerg, N. Rest before slaughter ameliorates pre-slaughter stress-induced increased drip loss but not stress-induced increase in the toughness of pork Meat Sci. 2009, 83, 634-641
10. Kuchenmeister, U.; Kuhn, G.; Stabenow, B.; Ender, K. The effect of experimental stress on sarcoplasmic reticulum Ca2+ transport and meat quality in pig muscle Meat Sci. 2002, 61, 375-380
11. Kuchenmeister, U.; Kuhn, G.; Ender, K. Preslaughter handling of pigs and the effect on heart rate, meat quality, including tenderness, and sarcoplasmic reticulum Ca2+ transport Meat Sci. 2005, 71, 690-695
12. Terlouw, C. Stress reactions at slaughter and meat quality in pigs: genetic background and prior experience-A brief review of recent Livest. Prod. Sci. 2005, 94, 125-135
13. Young, J. F.; Hansen-Moller, J.; Oksbjerg, N. Effect of flavonoids on stress responses in myotube cultures J. Agric. Food Chem. 2004, 52, 7158-7163
14. Gawlitta, D.; Oomens, C. W. J.; Bader, D. L.; Baaijens, F. P. T.; Bouten, C. V. C. Temporal differences in the influence of ischemic factors and deformation on the metabolism of engineered skeletal muscle J. Appl. Physiol. 2007, 103, 464-473
15. Siu, P. M.; Wang, Y.; Alway, S. E. Apoptotic signaling induced by H2O2-mediated oxidative stress in differentiated C2C12 myotubes Life Sci. 2009, 84, 468-481
16. Ortenblad, N.; Young, J. F.; Oksbjerg, N.; Nielsen, J. H.; Lambert, I. H. Reactive oxygen species are important mediators of taurine release from skeletal muscle cells Am. J. Physiol.-Cell Ph. 2003, 284, C1362-C1373
17. Straadt, I. K.; Young, J. F.; Petersen, B. O.; Duus, J. O.; Gregersen, N.; Bross, P.; Oksbjerg, N.; Bertram, H. C. Metabolic profiling of heat or anoxic stress in mouse C2C12 myotubes using multinuclear NMR spectroscopy. Metabolism 2010, DOI: 10.1016/j.metabol.2009.09.029.
18. Lambert, I. H.; Nielsen, J. H.; Andersen, H. J.; Ortenblad, N. Cellular model for induction of drip loss in meat J. Agric. Food Chem. 2001, 49, 4876-4883
19. Teague, C. R.; Dhabhar, F. S.; Barton, R. H.; Beckwith-Hall, B.; Powell, J.; Cobain, M.; Singer, B.; Mcewen, B. S.; Lindon, J. C.; Nicholson, J. K.; Holmes, E. Metabonomic studies on the physiological effects of acute and chronic psychological stress in Sprague-Dawley rats J. Proteome Res. 2007, 6, 2080-2093
20. Karakach, T. K.; Huenupi, E. C.; Soo, E. C.; Walter, J. A.; Afonso, L. O. B. H-1-NMR and mass spectrometric characterization of the metabolic response of juvenile Atlantic salmon (Salmo salar) to long-term handling stress Metabolomics 2009, 5, 123-137
21. Bertram, H. C.; Oksbjerg, N.; Young, J. F. NMR-based metabonomics reveals relationship between pre-slaughter exercise stress, the plasma metabolite profile at time of slaughter, and water-holding capacity in pigs Meat Sci. 2010, 84, 108-113
22. Coquin, L.; Feala, J. D.; McCulloch, A. D.; Paternostro, G. Metabolomic and flux-balance analysis of age-related decline of hypoxia tolerance in Drosophila muscle tissue Mol. Syst. Biol. 2008, 4, 1-13
23. Klont, R. E.; Talmant, A.; Monin, G. Effect of Temperature on Porcine-Muscle Metabolism Studied in Isolated Muscle-Fiber Strips Meat Sci. 1994, 38, 179-191
24. Febbraio, M. A. Does Muscle Function and Metabolism Affect Exercise Performance in the Heat? Exerc. Spotr. Sci. Rev. 2000, 28, 171-176
25. Bischoff, R. Enzymatic Liberation of Myogenic Cells from Adult Rat Muscle Anat. Rec. 1974, 180, 645-661
26. Theil, P. K.; Sorensen, I. L.; Nissen, P. M.; Oksbjerg, N. Temporal expression of growth factor genes of primary porcine satellite cells during myogenesis Anim. Sci. J. 2006, 77, 330-337
27. Yaffe, D.; Saxel, O. Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle Nature 1977, 270, 725-727
28. Bustin, S. A. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems J. Mol. Endocrinol. 2002, 29
29. Young, J. F.; Christensen, L. P.; Theil, P. K.; Oksbjerg, N. The Polyacetylenes Falcarinol and Falcarindiol Affect Stress Responses in Myotube Cultures in A Biphasic Manner Dose-Response 2008, 6, 239-251
30. Mayr, M.; Liem, D.; Zhang, J.; Li, X. H.; Avliyakuiov, N. K.; Yang, J. I.; Young, G.; Vondriska, T. M.; Ladroue, C.; Madhu, B.; Griffiths, J. R.; Gomes, A.; Xu, Q. B.; Ping, P. P. Proteomic and metabolomic analysis of cardioprotection: Interplay between protein kinase C epsilon and delta in regulating glucose metabolism of murine hearts J. Mol. Cell. Cardiol. 2009, 46, 268-277
31. Klingler, W.; Baur, C.; Georgieff, M.; Lehmann-Horn, F.; Melzer, W. Detection of proton release from cultured human myotubes to identify malignant hyperthermia susceptibility Anesthesiology 2002, 97, 1059-1066
32. Jensen-Waern, M.; Nyberg, L. Valuable Indicators of Physical Stress in Porcine Plasma J. Vet. Med. A 1993, 40, 321-327