Purification of His-tagged hepatitis B core antigen from unclarified bacterial homogenate using immobilized metal affinity-expanded bed adsorption chromatography

Journal of Chromatography A

Volumn 1217, Issue 21, 2010, Pages 3473-3480

  Yap, Wei Boon ^a   Tey, Beng Ti ^a   Alitheen, Noorjahan Banu Mohamed ^a   Tan, Wen Siang ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

Author keywords

His tagged HBcAg; IMA EBAC; Streamline Chelating; Unclarified bacterial homogenate; Virus like particles

Indexed keywords

ANTIGENICITY; BATCH ADSORPTION EXPERIMENTS; BINDING CAPACITIES; E. COLI; ELUTION CONDITIONS; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPANDED BED ADSORPTION; HEPATITIS B CORE ANTIGENS; HEPATITIS B VIRUS INFECTION; IMMOBILIZED METALS; OPTIMAL BINDING; PURIFICATION FACTORS; PURIFICATION PROCESS; VIRUS-LIKE PARTICLES;

ADSORPTION; ANTIGENS; BACTERIOLOGY; CHELATION; CHROMATOGRAPHY; COMPUTER VIRUSES; ESCHERICHIA COLI; METAL RECOVERY; OPTIMIZATION; PURIFICATION; SCANNING ELECTRON MICROSCOPY; SODIUM CHLORIDE; VIRUSES;

METAL REFINING;

ADSORBENT; AGAROSE; HEPATITIS B CORE ANTIGEN; HISTIDINE; IMIDAZOLE;

ADSORPTION; ANTIGEN PURIFICATION; ANTIGENICITY; ARTICLE; BINDING KINETICS; CONTROLLED STUDY; ELECTRON MICROSCOPY; ELUTION; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EXPANDED BED CHROMATOGRAPHY; HEPATITIS B VIRUS; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN ASSEMBLY; VIRUS LIKE AGENT;

ADSORPTION; CENTRIFUGATION, DENSITY GRADIENT; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HEPATITIS B CORE ANTIGENS; HISTIDINE; HYDROGEN-ION CONCENTRATION; IMIDAZOLES; MICROSCOPY, ELECTRON, TRANSMISSION; PARTICLE SIZE; RECOMBINANT PROTEINS; SODIUM CHLORIDE; SUCROSE; VISCOSITY;

BACTERIA (MICROORGANISMS); HEPATITIS B VIRUS;

EID: 77952553509     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2010.03.012     Document Type: Article

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