Comparative evaluation of three purification methods for the nucleocapsid protein of Newcastle disease virus from Escherichia coli homogenates

Journal of Microbiology

Volumn 43, Issue 3, 2005, Pages 295-300

  Yan, Peng Tan ^a   Tau, Chuan Ling ^a   Yusoff, Khatijah ^a   Wen, Siang Tan ^a   Beng, Ti Tey ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

Author keywords

Escherichia coli; Expanded bed adsorption; NDV; NP protein; Packed bed adsorption

Indexed keywords

CHELATING AGENT; NICKEL; NUCLEOPROTEIN; NUCLEOPROTEIN, NEWCASTLE DISEASE VIRUS; RECOMBINANT PROTEIN; VIRUS PROTEIN;

ADSORPTION; ARTICLE; BIOTECHNOLOGY; CHROMATOGRAPHY; COMPARATIVE STUDY; ESCHERICHIA COLI; EVALUATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; NEWCASTLE DISEASE PARAMYXOVIRUS;

ADSORPTION; BIOTECHNOLOGY; CHELATING AGENTS; CHROMATOGRAPHY; ESCHERICHIA COLI; NEWCASTLE DISEASE VIRUS; NICKEL; NUCLEOPROTEINS; RECOMBINANT PROTEINS; VIRAL PROTEINS;

ESCHERICHIA COLI; NEWCASTLE DISEASE VIRUS;

EID: 22244489361     PISSN: 12258873     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Ameskamp, N., C. Priesner, J. Lehmann, and D. Lutkemeyer. 1999. Pilot scale recovery of monoclonal antibodies by expanded bed ion exchange adsorption. Bioseparation 8, 169-88.
2. Anspach, F.B., D. Curbelo, R. Hartmann, G. Garke, and W.D. Deekwer. 1999. Expanded-bed chromatography in primary protein purification. J. Chromatogr. A 865, 129-144.
3. Batt, B.C., V.M. Yabannavar, and V. Singh. 1995. Expanded bed adsorption process for protein recovery from whole mammalian cell culture broth. Bioseparation 5, 41-52.
4. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
5. Chaga, GS. 2001. Twenty-five years of immobilized metal ion affinity chromatography: past, present and future. J. Biochem. Biophys. Methods 49, 313-334.
6. Chang, Y.K. and H.A. Chase. 1996. Ion exchange purification of G6PDH from unclarified yeast cell homogenates using expanded bed adsorption. Biotechnol. Bioeng. 49, 204-216.
7. Chase, H.A. and N.M. Draeger. 1992. Affinity purification of proteins using expanded beds. J. Chromatogr. A 597, 129-145.
8. Ersson, B., L. Ryden, and J.-C. Janson. 1998. Introduction to protein purification, p. 3-40. In J-C. Janson, L. Ryden (eds.), Protein Purification: Principles, High-Resolution Methods, and Applications. John Wiley & Sons, New York.
9. Hu, Y.C., C. T. Tsai, Y.C. Chung, J.T. Lu, and J.T. Hsu. 2003. Generation of chimeric baculovirus with histidine-tags displayed on the envelope and its purification using immobilized metal affinity chromatography. Enzyme Microb. Technol. 33, 445-452.
10. Kaufmann, M. 1997. Unstable proteins: how to subject them to chromatographic separations for purification procedures. J. Chromatogr. B 699, 347-369.
11. Kho, C.L., W.S. Tan, and K. Yusoff. 2001. Production of the nucleocapsid protein of Newcastle disease virus in Escherichia coli and its assembly into ring- and nucleocapsid-like particles. J. Microbiol. 39, 293-299.
12. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
13. Lee, S.M. 1989. The primary stages of protein recovery. J. Biotechnol. 11, 103-118.
14. Ling, T.C. 2002. Ph. D. thesis. The University of Birmingham, UK.
15. Ling, T.C., C.K. Long, W.S. Tan, B.T. Tey, A. Ariff, W.M. Wan Abdullah. 2004. Purification of filamentous bacteriophage M13 by expanded bed anion exchange chromatography. J. Micorbiol. 42, 22-232.
16. Noppe, W., I. Hanssens, and M.D. Cuyper. 1996. Simple two step procedure for the preparation of highly active pure equine milk lysozyme. J. Chromatogr. A 719, 327-331.
17. Rabu, A., W.S. Tan, C.L. Kho, A.R. Omar, and K. Yusoff. 2002. Chimeric Newcastle disease virus nucleocapsid with parts of its viral haemagglutinin-neuraminidase and fusion proteins. Acta Virol. 46, 211-217.
18. Sumi, A., K. Okuyama, K. Kobayashi, W. Ohtani, T. Ohmura, and K. Yokoyama. 1999. Purification of recombinant human serum albumin efficient purification using STREAMLINE. Bioseparation 8, 195-200.
19. Tey, B.T., K.H.Yong, H.P. Ong, T.C. Ling, S.T. Ong, Y.P. Tan, A. Ariff, and W.S. Tan. 2004. Optimal conditions for Hepatitis B core Antigen production in shaked flask fermentation. Biotechnol. Bioprocess Eng. 9, 374-378.
20. Thömmes, J., A. Bader, M. Halfar, A. Karau, and M-R Kula. 1996. Isolation of monoclonal antibodies from cell containing hybridoma broth using a protein A. coated adsorbent in expanded beds. J. Chromatogr. A 752, 111-122.
21. Thömmes, J., M. Halfar, S. Lenz, M.-R. Kula. 1995. Purification of monoclonal antibodies from whole hybridoma fermentation broth by fluidized bed adsorption. Biotechnol. Bioeng. 45, 205-211.
22. Ueda, E.K.M., P.W. Gout, and L. Morganti. 2003. Current and prospective applications of metal ion-protein binding. J. Chromatogr. A 988, 1-23.
23. Willoughby, N.A., T. Kirschner, M.P. Smith, R. Hjorth, and N.J. Titchener-Hooker. 1999. Immobilized metal affinity chromatography purification of alcohol dehydrogenase from Baker's yeast using an expanded bed adsorption system. J. Chromatogr. A 840, 195-204.
24. Xiang, H., R. Wynn, L-H. T. Nguyen, O.H. Ross, D.P. Ahrens, K.T. O'Neil, G.F. Hollis, and D.R. Patrick. 2002. Immobilized metal-ion affinity chromatography of recombinant Fab protein OPG C11 in the presence of EDTA-Mg (II). J. Chromatogr. A 978, 153-164.
25. Yusoff, K. and W.S. Tan. 2001. Newcastle disease virus: macromolecules and opportunities. Avian Pathol. 30, 439-455.