Large-scale production, bacterial localization assessment and immobilized metal affinity chromatography purification of a human single-chain Fv antibody against alphaIIb-beta3 integrin

International Journal of Biological Macromolecules

Volumn 39, Issue 1-3, 2006, Pages 51-59

  Robert, R ^a   Clofent Sanchez, G ^a   Hocquellet, A ^b   Jacobin Valat, M J ^a   Daret, D ^c   Noubhani, A M ^b   Santarelli, X ^b  

^a CNRS   (France)

^b UNIVERSITÉ DE BORDEAUX   (France)

^c INSERM   (France)

Author keywords

alphaIIb beta3 ( IIb 3); Fermentation; IMAC purification; ScFv

Indexed keywords

ALPHA2B BETA3 INTEGRIN; LYMPHOCYTE ANTIGEN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; ATHEROSCLEROSIS; CELL INCLUSION; CONTROLLED STUDY; CYTOPLASM; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EXPANDED BED CHROMATOGRAPHY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IMMUNOHISTOCHEMISTRY; MALE; NONHUMAN; PURIFICATION; RABBIT; THROMBUS; ULTRASOUND;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; ORYCTOLAGUS CUNICULUS; THROMBUS;

EID: 33746023963     PISSN: 01418130     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijbiomac.2006.01.014     Document Type: Article

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