The sodium/galactose symporter crystal structure is a dynamic, not so occluded state

Molecular BioSystems

Volumn 6, Issue 6, 2010, Pages 1040-1046

  Zomot, Elia ^a   Bahar, Ivet ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; COTRANSPORTER; GALACTOSE; SODIUM;

ARTICLE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; CYTOPLASM; KINETICS; METABOLISM; MOLECULAR DYNAMICS; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; TRANSPORT AT THE CELLULAR LEVEL; VIBRIO PARAHAEMOLYTICUS; X RAY CRYSTALLOGRAPHY;

BACTERIAL PROTEINS; BINDING SITES; BIOLOGICAL TRANSPORT; CRYSTALLOGRAPHY, X-RAY; CYTOPLASM; GALACTOSE; KINETICS; MODELS, MOLECULAR; MOLECULAR DYNAMICS SIMULATION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SODIUM; SYMPORTERS; VIBRIO PARAHAEMOLYTICUS;

BACTERIA (MICROORGANISMS); VIBRIO PARAHAEMOLYTICUS;

EID: 77952481518     PISSN: 1742206X     EISSN: 17422051     Source Type: Journal    
DOI: 10.1039/b927492h     Document Type: Article

References (30)

1. B. I. Kanner Bioenergetics of neurotransmitter transport Biochim. Biophys. Acta 1983 726 293 316
2. B. I. Kanner S. Schuldiner Mechanism of transport and storage of neurotransmitters Crit. Rev. Biochem. Mol. Biol. 1987 22 1 38
3. E. M. Wright D. D. Loo B. A. Hirayama E. Turk Surprising versatility of Na+-glucose cotransporters: SLC5 Physiology (Bethesda) 2004 19 370 376
4. D. W. Leung D. D. Loo B. A. Hirayama T. Zeuthen E. M. Wright Urea transport by cotransporters J. Physiol. 2000 528 2 251 257
5. M. J. Coady M. H. Chang F. M. Charron C. Plata B. Wallendorff J. F. Sah S. D. Markowitz M. F. Romero J. Y. Lapointe The human tumour suppressor gene SLC5A8 expresses a Na+-monocarboxylate cotransporter J. Physiol. 2004 557 719 731
6. H. Li L. Myeroff D. Smiraglia M. F. Romero T. P. Pretlow L. Kasturi J. Lutterbaugh R. M. Rerko G. Casey J. P. Issa J. Willis J. K. V. Willson C. Plass S. D. Markowitz SLC5A8 a sodium transporter is a tumor suppressor gene silenced by methylation in human colon aberrant crypt foci and cancers Proc. Natl. Acad. Sci. U. S. A. 2003 100 8412 8417
7. S. Faham A. Watanabe G. M. Besserer D. Cascio A. Specht B. A. Hirayama E. M. Wright J. Abramson The crystal structure of a sodium galactose transporter reveals mechanistic insights into Na+/sugar symport Science 2008 321 810 814
8. P. L. Shaffer A. Goehring A. Shankaranarayanan E. Gouaux Structure and mechanism of a Na+-independent amino acid transporter Science 2009 325 1010 1014
9. S. K. Singh C. L. Piscitelli A. Yamashita E. Gouaux A competitive inhibitor traps LeuT in an open-to-out conformation Science 2008 322 1655 1661
10. S. Weyand T. Shimamura S. Yajima S. Suzuki O. Mirza K. Krusong E. P. Carpenter N. G. Rutherford J. M. Hadden J. O'Reilly P. Ma M. Saidijam S. G. Patching R. J. Hope H. T. Norbertczak P. C. J. Roach S. Iwata P. J. F. Henderson A. D. Cameron Structure and molecular mechanism of a nucleobase-cation-symport- 1 family transporter Science 2008 322 709 713
11. A. Yamashita S. K. Singh T. Kawate Y. Jin E. Gouaux Crystal structure of a bacterial homologue of Na+/Cl - dependent neurotransmitter transporters Nature 2005 437 215 223
12. X. Gao F. Lu L. Zhou S. Dang L. Sun X. Li J. Wang Y. Shi Structure and mechanism of an amino acid antiporter Science 2009 324 1565 1568
13. S. Ressl A. C. Terwisscha van Scheltinga C. Vonrhein V. Ott C. Ziegler Molecular basis of transport and regulation in the Na(+)/betaine symporter BetP Nature 2009 458 47 52
14. S. K. Singh C. L. Piscitelli A. Yamashita E. Gouaux A competitive inhibitor traps LeuT in an open-to-out conformation Science 2008 322 1655 1661
15. H. Krishnamurthy C. L. Piscitelli E. Gouaux Unlocking the molecular secrets of sodium-coupled transporters Nature 2009 459 347 355
16. T. S. Ikeda E. S. Hwang M. J. Coady B. A. Hirayama M. A. Hediger E. M. Wright Characterization of a Na+/glucose cotransporter cloned from rabbit small intestine J. Membr. Biol. 1989 110 87 95
17. Z. Xie E. Turk E. M. Wright Characterization of the Vibrio parahaemolyticus Na+/Glucose cotransporter. A bacterial member of the sodium/glucose transporter (SGLT) family J. Biol. Chem. 2000 275 25959 25964
18. E. Turk O. Kim C. J. le J. P. Whitelegge S. Eskandari J. T. Lam M. Kreman G. Zampighi K. F. Faull E. M. Wright Molecular characterization of Vibrio parahaemolyticus vSGLT: a model for sodium-coupled sugar cotransporters J. Biol. Chem. 2000 275 25711 25716
19. S. Izrailev S. Stepaniants M. Balsera Y. Oono K. Schulten Molecular dynamics study of unbinding of the avidin-biotin complex Biophys. J. 1997 72 1568 1581 (Pubitemid 27133097)
20. D. Kosztin S. Izrailev K. Schulten Unbinding of retinoic acid from its receptor studied by steered molecular dynamics Biophys. J. 1999 76 188 197
21. S. Eskandari E. M. Wright M. Kreman D. M. Starace G. A. Zampighi Structural analysis of cloned plasma membrane proteins by freeze-fracture electron microscopy Proc. Natl. Acad. Sci. U. S. A. 1998 95 11235 11240
22. L. Celik B. Schiott E. Tajkhorshid Substrate binding and formation of an occluded state in the leucine transporter Biophys. J. 2008 94 1600 1612
23. D. A. Caplan J. O. Subbotina S. Y. Noskov Molecular mechanism of ion-ion and ion-substrate coupling in the Na+-dependent leucine transporter LeuT Biophys. J. 2008 95 4613 4621
24. J. Li E. Tajkhorshid Ion-releasing state of a secondary membrane transporter Biophys. J. 2009 97 L29 L31
25. H. M. Berman J. Westbrook Z. Feng G. Gilliland T. N. Bhat H. Weissig I. N. Shindyalov P. E. Bourne The Protein Data Bank Nucleic Acids Res. 2000 28 235 242
26. W. Humphrey A. Dalke K. Schulten VMD: visual molecular dynamics J. Mol. Graphics 1996 14 33 38
27. A. D. Mackerell, Jr. M. Feig C. L. Brooks, III Extending the treatment of backbone energetics in protein force fields: limitations of gas-phase quantum mechanics in reproducing protein conformational distributions in molecular dynamics simulations J. Comput. Chem. 2004 25 1400 1415
28. A. D. Mackerell, Jr. Empirical force fields for biological macromolecules: overview and issues J. Comput. Chem. 2004 25 1584 1604
29. J. C. Phillips R. Braun W. Wang J. Gumbart E. Tajkhorshid E. Villa C. Chipot R. D. Skeel L. Kale K. Schulten Scalable molecular dynamics with NAMD J. Comput. Chem. 2005 26 1781 1802
30. B. R. Brooks et al., CHARMM: the biomolecular simulation program J. Comput. Chem. 2009 30 1545 1614