Evaluation of cartilage specific matrix synthesis of human articular chondrocytes after extended propagation on microcarriers by image analysis

International Journal of Artificial Organs

Volumn 33, Issue 4, 2010, Pages 204-218

  Goepfert, Christiane ^a   Lutz, Vivien ^a   Lünse, Svenja ^a   Kittel, Sabrina ^a   Wiegandt, Katharina ^a   Kammal, Michael ^b   Püschel, Klaus ^b   Pörtner, Ralf ^a  

^a HAMBURG UNIVERSITY OF TECHNOLOGY   (Germany)

^b UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

Author keywords

Bead to bead transfer; Cartilage repair; Image analysis; Microcarriers; Redifferentiation of chondrocytes

Indexed keywords

BODY FLUIDS; CARTILAGE; CELLS; COLLAGEN; CYTOLOGY; MATRIX ALGEBRA; REPAIR;

BEAD-TO-BEAD TRANSFER; CARTILAGE REPAIR; CHONDROCYTES; COLLAGEN TYPE I; HUMAN ARTICULAR CHONDROCYTES; IMAGE-ANALYSIS; MATRIX SYNTHESIS; MICROCARRIERS; REDIFFERENTIATION OF CHONDROCYTE; TYPE II;

IMAGE ANALYSIS;

ALGINIC ACID; COLLAGEN TYPE 1; COLLAGEN TYPE 2; OSTEOGENIC PROTEIN 1; SOMATOMEDIN C; TRANSFORMING GROWTH FACTOR BETA;

ARTICLE; CARTILAGE CELL; CARTILAGE MATRIX; CELL DEDIFFERENTIATION; CELL EXPANSION; CELL GROWTH; CELL PROLIFERATION; CHONDROGENESIS; COLLAGEN SYNTHESIS; CONTROLLED STUDY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMAGE ANALYSIS; MICROCARRIER CULTURE; MONOLAYER CULTURE; OXYGEN CONCENTRATION;

EID: 77952417756     PISSN: 03913988     EISSN: None     Source Type: Journal    
DOI: 10.1177/039139881003300405     Document Type: Article

References (53)

1. Petersen JP, Ruecker A, Stechow D von, et al. Present and future therapies of articular cartilage defects. Eur J Trauma 2003; 1-10. (Pubitemid 36262501)
2. Roberts S, Menage J, Sandell LJ, Evans EH, Richardson JB. Immunohistochemical study of collagen types I and II and procollagen IIA in human cartilage repair tissue following autologous chondrocyte implantation. Knee 2009; 16: 398-404.
3. Jakob M, Démarteau O, Schäfer D, et al. Specific growth factors during the expansion and redifferentiation of adult human articular chondrocytes enhance chondrogenesis and cartilaginous tissue formation in vitro. J Cell Biochem 2001; 81: 368-377 (Pubitemid 32298268)
4. Malda J, Frondoza CG. Microcarriers in the engineering of cartilage and bone. Trends Biotechnol 2006; 24: 299-304.
5. Malda J, Kreijveld E, Temenoff JS, van Blitterswijk CA, Riesle J. Expansion of human nasal chondrocytes on macroporous microcarriers enhances redifferentiation. Biomaterials 2003; 24: 153-161 (Pubitemid 37272280)
6. Malda J, van Blitterswijk CA, Grojec M, Martens DE, Tramper J, Riesle J. Expansion of bovine chondrocytes on microcarriers enhances redifferentiation. Tissue Eng 2003; 9: 39-48.
7. Malda J, van den Brink P, Meeuwse P, et al. Effect of oxygen tension on adult articular chondrocytes in microcarrier bioreactor culture. Tissue Eng 2004; 10: 87-94.
8. Martin I, Vunjak-Novakovic G, Yang J, Langer R, Freed LE. Mammalian chondrocytes expanded in the presence of fibroblast growth factor 2 maintain the ability to differentiate and regenerate three-dimensional cartilaginous tissue. Exp Cell Res 1999; 253: 81-88
9. Mandelbaum B, Browne JE, Fu F, et al. Treatment outcomes of autologous chondrocyte implantation for full-thickness articular cartilage defects of the trochlea. Am J Sports Med 2007; 35: 15-21.
10. Jakob M, Démarteau O, Schäfer D, Stumm M, Heberer M, Martin I. Enzymatic digestion of adult human articular cartilage yields a small fraction of the total available cells. Connect Tissue Res 2003; 44: 73-80.
11. Pei M, Seidel J, Vunjak-Novakovic G, Freed LE. Growth factors for sequential cellular de- and re-differentiation in tissue engineering. Biochem Biophys Res Commun 2002; 294: 49-54.
12. Martin I, Muraglia A, Campanile G, Cancedda R, Quarto R. Fibroblast growth factor-2 supports ex vivo expansion and maintenance of osteogenic precursors from human bone marrow. Endocrinology 1997; 138: 456-462
13. Pörtner R, Goepfert C, Wiegandt K, et al. Technical strategies to improve tissue engineering of cartilage-carrier-constructs. Adv Biochem Eng Biotechnol 2009; 112: 45-81.
14. Malda J, Martens DE, Tramper J, van Blitterswijk CA, Riesle J. Cartilage tissue engineering; controversy in the effect of oxygen. Crit Rev Biotechnol 2003; 23: 75-94.
15. Domm C, Schünke M, Christesen K, Kurz B. Redifferentiation of dedifferentiated bovine articular chondrocytes in alginate culture under low oxygen tension. Osteoarthr Cartil 2002; 10: 3-22.
16. O'Driscoll SW, Fitzsimmons JS, Commisso CN. Role of oxygen tension during cartilage formation by periosteum. J Orthop Res 1997; 15: 82-87
17. Wernike E, Li Z, Alini M, Grad S. Effect of reduced oxygen tension and long-term mechanical stimulation on chondrocyte-polymer constructs. Cell Tissue Res 2008; 331: 73-83.
18. Nagel-Heyer S, Goepfert C, Adamietz P, Meenen NM, Pörtner R. Cultivation of three-dimensional cartilage-carrier-constructs under reduced oxygen tension. J Biotechnol 2006; 121: 86-97.
19. Benz K, Breit S, Lukoschek M, Mau H, Richter W. Molecular analysis of expansion, differentiation, and growth factor treatment of human chondrocytes identifies differentiation markers and growth-related genes. Biochem Biophys Res Commun 2002; 293: 84-92.
20. Blunk T, Sieminski AL, Gooch KJ, et al. Differential effects of growth factors on tissue-engineered cartilage. Tissue Eng 2002; 8: 3-84.
21. Francioli S, Martin I, Sie C, et al. Growth factors for clinicalscale expansion of human articular chondrocytes; relevance for automated bioreactor systems. Tissue Eng 2007; 13: 227-234
22. Wiegandt K, Goepfert C, Pörtner R. Improving in vitro generated cartilage-carrier-constructs by optimizing growth factor combination. Open Biomed Eng J 2007; 1: 5-90.
23. Heywood HK, Bader DL, Lee DA. Glucose concentration and medium volume influence cell viability and glycosaminoglycan synthesis in chondrocyte-seeded alginate constructs. Tissue Eng 2006; 12: 487-496
24. Guerne PA, Blanco F, Kaelin A, Desgeorges A, Lotz M. Growth factor responsiveness of human articular chondrocytes in aging and development. Arthritis Rheum 1995; 38: 60-68
25. Doré S, Pelletier JP, DiBattista JA, Tardif G, Brazeau P, Martel-Pelletier J. Human osteoarthritic chondrocytes possess an increased number of insulin-like growth factor 1 binding sites but are unresponsive to its stimulation. Possible role of IGF-1-binding proteins. Arthritis Rheum 1994; 37: 53-63.
26. Hsieh-Bonassera ND, Wu I, Lin JK, et al. Expansion and redifferentiation of chondrocytes from osteoarthritic cartilage; cells for human cartilage tissue engineering. Tissue Eng Part A 2009; 15: 513-523
27. Glansbeek HL, van der Kraan PM, Vitters EL, van den Berg WB. Variable TGF-beta receptor expression regulates TGF-beta responses of articular chondrocytes. Agents Actions Suppl 1993; 39: 39-45.
28. Guerne PA, Sublet A, Lotz M. Growth factor responsiveness of human articular chondrocytes; distinct profiles in primary chondrocytes, subcultured chondrocytes, and fibroblasts. J Cell Physiol 1994; 158: 76-84.
29. Haart M de, Marijnissen WJ, van Osch GJ, Verhaar JA. Optimization of chondrocyte expansion in culture. Effect of TGF beta-2, bFGF and L-ascorbic acid on bovine articular chondrocytes. Acta Orthop Scand 1999; 70: 5-61.
30. Vincent TL, Hermansson MA, Hansen UN, Amis AA, Saklatvala J. Basic fibroblast growth factor mediates transduction of mechanical signals when articular cartilage is loaded. Arthritis Rheum 2004; 50: 26-33.
31. Mandl EW, van der Veen SW, Verhaar JAN, van Osch GJVM. Serum-free medium supplemented with high-concentration FGF2 for cell expansion culture of human ear chondrocytes promotes redifferentiation capacity. Tissue Eng 2002; 8: 73-80.
32. Mandl EW, Jahr H, Koevoet JLM, van Leeuwen JPTM, Weinans H, Verhaar JAN, van Osch GJVM. Fibroblast growth factor- 2 in serum-free medium is a potent mitogen and reduces dedifferentiation of human ear chondrocytes in monolayer culture. Matrix Biol 2004; 23: 31-41. (Pubitemid 39013981)
33. Barbero A, Ploegert S, Heberer M, Martin I. Plasticity of clonal populations of dedifferentiated adult human articular chondrocytes. Arthritis Rheum 2003; 48: 315-325 (Pubitemid 36554530)
34. Luyten FP, Hascall VC, Nissley SP, Morales TI, Reddi AH. Insulin-like growth factors maintain steady-state metabolism of proteoglycans in bovine articular cartilage explants. Arch Biochem Biophys 1988; 267: 16-25.
35. Matsumoto T, Gargosky SE, Iwasaki K, Rosenfeld RG. Identifi- cation and characterization of insulin-like growth factors (IGFs), IGF-binding proteins (IGFBPs), and IGFBP proteases in human synovial fluid. J Clin Endocrinol Metab 1996; 81: 50-55 (Pubitemid 26035017)
36. Nixon AJ, Saxer RA, Brower-Toland BD. Exogenous insulin-like growth factor-I stimulates an autoinductive IGF-I autocrine/ paracrine response in chondrocytes. J Orthop Res 2001; 19: 6-32.
37. Morales TI, Roberts AB. Transforming growth factor beta regulates the metabolism of proteoglycans in bovine cartilage organ cultures. J Biol Chem 1988; 263: 2828-2831
38. Morales TI. Transforming growth factor-beta 1 stimulates synthesis of proteoglycan aggregates in calf articular cartilage organ cultures. Arch Biochem Biophys 1991; 286: 9-106.
39. Morales TI, Joyce ME, Sobel ME, Danielpour D, Roberts AB. Transforming growth factor-beta in calf articular cartilage organ cultures; synthesis and distribution. Arch Biochem Biophys 1991; 288: 97-405.
40. Redini F, Galera P, Mauviel A, Loyau G, Pujol JP. Transforming growth factor beta stimulates collagen and glycosaminoglycan biosynthesis in cultured rabbit articular chondrocytes. FEBS Lett 1988; 234: 72-76
41. van Osch GJ, van der Veen SW, Buma P, Verwoerd-Verhoef HL. Effect of transforming growth factor-beta on proteoglycan synthesis by chondrocytes in relation to differentiation stage and the presence of pericellular matrix. Matrix Biol 1998; 17: 13-24.
42. van Osch GJ, van den Berg WB, Hunziker EB, Häuselmann HJ. Differential effects of IGF-1 and TGF beta-2 on the assembly of proteoglycans in pericellular and territorial matrix by cultured bovine articular chondrocytes. Osteoarthr Cartil 1998; 6: 87-95.
43. Tsukazaki T, Usa T, Matsumoto T, et al. Effect of transforming growth factor-beta on the insulin-like growth factor-I autocrine/ paracrine axis in cultured rat articular chondrocytes. Exp Cell Res 1994; 215: 9-16. (Pubitemid 24339166)
44. Yaeger PC, Masi TL, Ortiz JL de, Binette F, Tubo R, McPherson JM. Synergistic action of transforming growth factorbeta and insulin-like growth factor-I induces expression of type II collagen and aggrecan genes in adult human articular chondrocytes. Exp Cell Res 1997; 237: 18-25. (Pubitemid 28027926)
45. Flechtenmacher J, Huch K, Thonar EJ, et al. Recombinant human osteogenic protein 1 is a potent stimulator of the synthesis of cartilage proteoglycans and collagens by human articular chondrocytes. Arthritis Rheum 1996; 39: 896-904. (Pubitemid 26378582)
46. Huch K, Wilbrink B, Flechtenmacher J, et al. Effects of recombinant human osteogenic protein 1 on the production of proteoglycan, prostaglandin E2, and interleukin-1 receptor antagonist by human articular chondrocytes cultured in the presence of interleukin-1beta. Arthritis Rheum 1997; 40: 157-161 (Pubitemid 28016411)
47. Chubinskaya S, Hurtig M, Rueger DC. OP-1/BMP-7 in cartilage repair. Int Orthop 2007; 31: 73-81.
48. Chubinskaya S, Hakimiyan A, Pacione C, et al. Synergistic effect of IGF-1 and OP-1 on matrix formation by normal and OA chondrocytes cultured in alginate beads. Osteoarthr Cartil 2007; 15: 21-30. (Pubitemid 46381567)
49. Nagel-Heyer S, Goepfert C, Feyerabend F, et al. Bioreactor cultivation of three-dimensional cartilage-carrier-constructs. Bioprocess Biosyst Eng 2005; 27: 73-80.
50. Petersen JP, Ueblacker P, Goepfert C, et al. Long term results after implantation of tissue engineered cartilage for the treatment of osteochondral lesions in a minipig model. J Mater Sci Mater Med 2008; 19: 29-38. (Pubitemid 351436310)
51. Loeser RF, Todd MD, Seely BL. Prolonged treatment of human osteoarthritic chondrocytes with insulin-like growth factor-I stimulates proteoglycan synthesis but not proteoglycan matrix accumulation in alginate cultures. J Rheumatol 2003; 30: 565-570
52. Moretti M, Wendt D, Dickinson SC, et al. Effects of in vitro preculture on in vivo development of human engineered cartilage in an ectopic model. Tissue Eng 2005; 11: 421-428 (Pubitemid 41636428)
53. Im H, Pacione C, Chubinskaya S, van Wijnen AJ, Sun Y, Loeser RF. Inhibitory effects of insulin-like growth factor-1 and osteogenic protein-1 on fibronectin fragment- and interleukin- 1beta-stimulated matrix metalloproteinase-13 expression in human chondrocytes. J Biol Chem 2003; 278: 5386-5394 (Pubitemid 36835288)