Redifferentiation of dedifferentiated bovine articular chondrocytes in alginate culture under low oxygen tension

Osteoarthritis and Cartilage

Volumn 10, Issue 1, 2002, Pages 13-22

  Domm, C ^a   Schunke M ^a   Christesen, K ^a   Kurz, B ^a  

^a UNIVERSITY OF KIEL   (Germany)

Author keywords

Alginate; Articular cartilage; Collagen; Dedifferentiation; Glycosaminoglycans; Oxygen tension; Redifferentiation

Indexed keywords

ALGINIC ACID; CHONDROITIN 4 SULFATE; CHONDROITIN 6 SULFATE; COLLAGEN TYPE 1; COLLAGEN TYPE 2; COLLAGEN TYPE 9; KERATAN SULFATE; OXYGEN; PROLINE; TRITIUM;

AMINO ACID METABOLISM; ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CARTILAGE CELL; CARTILAGE MATRIX; CATTLE; CELL ACTIVITY; CELL DIFFERENTIATION; CELL DIVISION; CELL ISOLATION; CELL VIABILITY; COLLAGEN SYNTHESIS; CONTROLLED STUDY; ENZYMATIC ASSAY; EXTRACELLULAR MATRIX; IMMUNOCYTOCHEMISTRY; MONOLAYER CULTURE; NONHUMAN; OXYGEN TENSION; PRIORITY JOURNAL; PROTEIN EXPRESSION; WESTERN BLOTTING;

EID: 0036158388     PISSN: 10634584     EISSN: None     Source Type: Journal    
DOI: 10.1053/joca.2001.0477     Document Type: Article

References (40)

1. The biological effect of continious passive motion on the healing of full-thickness defects in articular cartilage. An experimental investigation in the rabbit
2. Spongiolization: A new treatment for diseased patellae
3. A method of resurfacing osteoarthritic knee joints
4. Biologic resurfacing of a major joint defect with cryopreserved allogenetic periosteum under the influence of continuous passive motion in a rabbit model
5. Bone and cartilage allotransplantation. A review of 14 years of research and clinical studies
6. Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation
7. Proliferative potential of nasal septum chondrocytes for in vitro culture of cartilage transplants
8. Expression of mRNAs for collagens and other matrix components in dedifferentiating and redifferentiating human chondrocytes in culture
9. Collagen expression in embryonic chicken chondrocytes treated with phorbol myristate acetate
10. Changes in type of collagen synthesized as clones of chick chondrocytes grow and eventually lose division capacity
11. The progeny of rabbit articular chondrocytes synthesize collagen types I and II and type I trimer, but not type II. Verification by cyanogen bromide peptide analysis
12. Independent regulation of collagen types by chondrocytes during the loss of dedifferentiated function in culture
13. Transforming growth factor-beta 1 stimulates type II collagen expression in cultured periosteum-derived cells
14. Transforming growth factor-beta 1 stimulates chondrogenesis and inhibits osteogenesis in high density culture of periosteum-derived cells
15. Differentiation of mesenchymal limb bud cells to chondrocytes in alginate beads
16. Characterization of crosslinked collagens synthesized by mature articular chondrocytes cultured in alginate beads: Comparison of two distinct matrix compartments
17. Expression of a stable articular cartilage phenotype without evidence of hypertrophy by adult human articular chondrocytes in vitro
18. Re-expression of differentiated proteoglycan phenotype by dedifferentiated human chondrocytes during culture in alginate beads
19. Dedifferentiated chondrocytes cultured in alginate beads: Restoration of the differentiated phenotype and of the metabolic responses to interleukin-1 beta
20. Reexpression of cartilage-specific genes by dedifferentiated human articular chondrocytes cultured in alginate beads
21. Metabolism of Cartilage
22. Measurement of pH and ionic composition of pericellular sites
23. Transport through articular cartilage
24. Bovine articular chondrocyte function in vitro depends upon oxygen tension
25. Control of cartilage differentiation
26. Effects of thyroxine and low oxygen tension on chondrogenic expression in cell culture
27. Influence of oxygen concentration and mechanical factors on differentiation of connective tissues in vitro
28. Adaptation of chondrocytes to low oxygen tension: Relationship between hypoxia and cellular metabolism
29. Slowing down ageing of cultured embryonal chick chondrocytes by maintenance under low oxygen tension
30. Studies on synthesis and assembly of cartilage proteoglycan
31. Effects of alginate composition on the metabolic, secretory, and growth characteristics of entrapped beta TC3 mouse insulinoma cells
32. Effects of donors age on growth kinetics of rabbit articular chondrocytes in culture
33. Observations on the senescence of cells derived from articular cartilage
34. Age-related changes in the turnover of proteoglycans from explants of bovine articular cartilage
35. Age-related changes in the synthesis of matrix macromolecules by bovine articular cartilage
36. Effect of seeding density on stability of the dedifferentiated phenotype of pig articular chondrocytes in culture
37. Measurement of the intracellular concentration of oxygen in a cell perfusion system
38. Oxygen diffusivity in calcium alginate gel beads containing Gluconobacter suboxydans
39. Phenotypic stability of bovine articular chondrocytes after long-term culture in alginate beads
40. Synthesis of collagen by bovine chondrocytes cultured in alginate; posttranslational modifications and cell-matrix interaction