The Longus type IV pilus of enterotoxigenic Escherichia coli (ETEC) mediates bacterial self-aggregation and protection from antimicrobial agents

Microbial Pathogenesis

Volumn 48, Issue 6, 2010, Pages 230-238

  Clavijo, Andrea P ^a   Bai, Jing ^a   Gómez Duarte, Oscar G ^a  

^a Korea National Institute of Health   (South Korea)

Author keywords

Diarrhea; Enterotoxigenic E. coli; Longus; Mutagenesis; Pili

Indexed keywords

GENE PRODUCT; PROTEIN LNGA; UNCLASSIFIED DRUG;

AGGLUTINATION TEST; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL COLONIZATION; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM LONGUS TYPE PILUS; BACTERIUM PILUS; CONTROLLED STUDY; ELECTRON MICROSCOPY; ENTEROTOXIGENIC ESCHERICHIA COLI; GENE EXPRESSION; IMMUNOBLOTTING; IN VITRO STUDY; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; SURVIVAL RATE; WILD TYPE;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; BACTERIAL ADHESION; DNA, BACTERIAL; ENTEROTOXIGENIC ESCHERICHIA COLI; FIMBRIAE, BACTERIAL; MICROBIAL VIABILITY; MICROSCOPY, ELECTRON, TRANSMISSION; MOLECULAR SEQUENCE DATA; MURAMIDASE; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; VIBRIO CHOLERAE;

EID: 77952241054     PISSN: 08824010     EISSN: 10961208     Source Type: Journal    
DOI: 10.1016/j.micpath.2010.03.006     Document Type: Article

References (32)

1. Arnold K., Bordoli L., Kopp J., Schwede T. The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics 2006, 22:195-201.
2. Bieber D., Ramer S.W., Wu C.Y., Murray W.J., Tobe T., Fernandez R., et al. Type IV pili, transient bacterial aggregates, and virulence of enteropathogenic Escherichia coli. Science 1998, 280:2114-2118.
3. Cherepanov P.P., Wackernagel W. Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene 1995, 158:9-14.
4. Costerton J.W., Stewart P.S., Greenberg E.P. Bacterial biofilms: a common cause of persistent infections. Science 1999, 284:1318-1322.
5. Craig L., Taylor R.K., Pique M.E., Adair B.D., Arvai A.S., Singh M., et al. Type IV pilin structure and assembly: X-ray and EM analyses of Vibrio cholerae toxin-coregulated pilus and Pseudomonas aeruginosa PAK pilin. Mol Cell 2003, 11:1139-1150.
6. Datsenko K.A., Wanner B.L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 2000, 97:6640-6645.
7. Folkhard W., Marvin D.A., Watts T.H., Paranchych W. Structure of polar pili from Pseudomonas aeruginosa strains K and O. J Mol Biol 1981, 149:79-93.
8. Giron J.A., Gomez-Duarte O.G., Jarvis K.G., Kaper J.B. Longus pilus of enterotoxigenic Escherichia coli and its relatedness to other type-4 pili - a minireview. Gene 1997, 192:39-43.
9. Giron J.A., Ho A.S., Schoolnik G.K. An inducible bundle-forming pilus of enteropathogenic Escherichia coli. Science 1991, 254:710-713.
10. Giron J.A., Levine M.M., Kaper J.B. Longus: a long pilus ultrastructure produced by human enterotoxigenic Escherichia coli. Mol Microbiol 1994, 12:71-82.
11. Giron J.A., Viboud G.I., Sperandio V., Gomez-Duarte O.G., Maneval D.R., Albert M.J., et al. Prevalence and association of the longus pilus structural gene (lngA) with colonization factor antigens, enterotoxin types, and serotypes of enterotoxigenic Escherichia coli. Infect Immun 1995, 63:4195-4198.
12. Gomez-Duarte O.G., Chattopadhyay S., Weissman S.J., Giron J.A., Kaper J.B., Sokurenko E.V. Genetic diversity of the gene cluster encoding longus, a type IV pilus of enterotoxigenic Escherichia coli. J Bacteriol 2007, 189:9145-9149.
13. Gomez-Duarte O.G., Ruiz-Tagle A., Gomez D.C., Viboud G.I., Jarvis K.G., Kaper J.B., et al. Identification of lngA, the structural gene of longus type IV pilus of enterotoxigenic Escherichia coli. Microbiology 1999, 145(Pt 7):1809-1816.
14. Gutierrez-Cazarez Z., Qadri F., Albert M.J., Giron J.A. Identification of enterotoxigenic Escherichia coli harboring longus type IV pilus gene by DNA amplification. J Clin Microbiol 2000, 38:1767-1771.
15. Guzman L.M., Belin D., Carson M.J., Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol 1995, 177:4121-4130.
16. Hyland R.M., Griener T.P., Mulvey G.L., Kitov P.I., Srivastava O.P., Marcato P., et al. Basis for N-acetyllactosamine-mediated inhibition of enteropathogenic Escherichia coli localized adherence. J Med Microbiol 2006, 55:669-675.
17. Hyland R.M., Sun J., Griener T.P., Mulvey G.L., Klassen J.S., Donnenberg M.S., et al. The bundlin pilin protein of enteropathogenic Escherichia coli is an N-acetyllactosamine-specific lectin. Cell Microbiol 2008, 10:177-187.
18. Knutton S., Shaw R.K., Anantha R.P., Donnenberg M.S., Zorgani A.A. The type IV bundle-forming pilus of enteropathogenic Escherichia coli undergoes dramatic alterations in structure associated with bacterial adherence, aggregation and dispersal. Mol Microbiol 1999, 33:499-509.
19. Kosek M., Bern C., Guerrant R.L. The global burden of diarrhoeal disease, as estimated from studies published between 1992 and 2000. Bull World Health Organ 2003, 81:197-204.
20. Levine M.M., Ristaino P., Marley G., Smyth C., Knutton S., Boedeker E., et al. Coli surface antigens 1 and 3 of colonization factor antigen II-positive enterotoxigenic Escherichia coli: morphology, purification, and immune responses in humans. Infect Immun 1984, 44:409-420.
21. Mundy R., Pickard D., Wilson R.K., Simmons C.P., Dougan G., Frankel G. Identification of a novel type IV pilus gene cluster required for gastrointestinal colonization of Citrobacter rodentium. Mol Microbiol 2003, 48:795-809.
22. Nishimura L.S., Giron J.A., Nunes S.L., Guth B.E. Prevalence of enterotoxigenic Escherichia coli strains harboring the longus pilus gene in Brazil. J Clin Microbiol 2002, 40:2606-2608.
23. Park H.S., Wolfgang M., van Putten J.P., Dorward D., Hayes S.F., Koomey M. Structural alterations in a type IV pilus subunit protein result in concurrent defects in multicellular behaviour and adherence to host tissue. Mol Microbiol 2001, 42:293-307.
24. Pichel M.G., Binsztein N., Qadri F., Giron J.A. Type IV longus pilus of enterotoxigenic Escherichia coli: occurrence and association with toxin types and colonization factors among strains isolated in Argentina. J Clin Microbiol 2002, 40:694-697.
25. Sambrook J., Russell D.W. Molecular cloning a laboratory manual 2001, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 3rd ed.
26. Scaletsky I.C., Silva M.L., Trabulsi L.R. Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells. Infect Immun 1984, 45:534-536.
27. Schwede T., Kopp J., Guex N., Peitsch M.C. SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Res 2003, 31:3381-3385.
28. Strom M.S., Lory S. Structure-function and biogenesis of the type IV pili. Annu Rev Microbiol 1993, 47:565-596.
29. Taniguchi T., Akeda Y., Haba A., Yasuda Y., Yamamoto K., Honda T., et al. Gene cluster for assembly of pilus colonization factor antigen III of enterotoxigenic Escherichia coli. Infect Immun 2001, 69:5864-5873.
30. Taniguchi T., Fujino Y., Yamamoto K., Miwatani T., Honda T. Sequencing of the gene encoding the major pilin of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli and evidence that CFA/III is related to type IV pili. Infect Immun 1995, 63:724-728.
31. Tobe T., Sasakawa C. Role of bundle-forming pilus of enteropathogenic Escherichia coli in host cell adherence and in microcolony development. Cell Microbiol 2001, 3:579-585.
32. Vanmaele R.P., Armstrong G.D. Effect of carbon source on localized adherence of enteropathogenic Escherichia coli. Infect Immun 1997, 65:1408-1413.