Ribosome display and selection of human anti-placental growth factor scFv derived from ovarian cancer patients

Protein and Peptide Letters

Volumn 17, Issue 5, 2010, Pages 585-590

  Li, Fang ^a   Su, Pingping ^a   Lin, Chao ^a   Li, Hong ^a   Cheng, Jiajing ^a   Shi, Donglu ^a,b,c  

^a TONGJI UNIVERSITY   (China)

^b UNIVERSITY OF CINCINNATI   (United States)

^c SHANGHAI JIAO TONG UNIVERSITY   (China)

Author keywords

Antibody engineering; In vitro selection; Ovarian cancer; PlGF; Ribosome display; scFv

Indexed keywords

PLACENTA PROTEIN; PLACENTAL GROWTH FACTOR; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

AGAR GEL ELECTROPHORESIS; ARTICLE; CHEMISTRY; DRUG ANTAGONISM; FEMALE; GENE LIBRARY; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MONONUCLEAR CELL; OVARY TUMOR; PROTEIN BINDING; PROTEIN ENGINEERING; RIBOSOME; WESTERN BLOTTING;

BLOTTING, WESTERN; ELECTROPHORESIS, AGAR GEL; FEMALE; GENE LIBRARY; HUMANS; LEUKOCYTES, MONONUCLEAR; OVARIAN NEOPLASMS; PREGNANCY PROTEINS; PROTEIN BINDING; PROTEIN ENGINEERING; RIBOSOMES; SINGLE-CHAIN ANTIBODIES;

EID: 77951981938     PISSN: 09298665     EISSN: None     Source Type: Journal    
DOI: 10.2174/092986610791112729     Document Type: Article

References (21)

1. Kohler, G.; Milstein, C. Continuous cultures of fused cells secreting antibody antigen interactions. Nature, 1975, 256(5517), 495-497.
2. Hanes, J.; Plückthun, A. In vitro selection and evolution of functional proteins by using ribosome display. Proc. Natl. Acad. Sci. USA, 1997, 94(10), 4937-4942.
3. Winter, G.; Griffiths, A.D.; Hawkins, R.E.; Hoogenboom, H. R. Making antibodies by phage display technology. Annu. Rev. Immunol., 1994, 12, 433-455.
4. He, M.; Menges, M.; Groves, M.A.; Corps, E.; Liu, H.; Brüggemann, M. Selection of a human anti-progesterone antibody fragment from a transgenic mouse library by ARM ribosome display. J. Immunol. Methods, 1999, 231(1-2), 105-117.
5. Amstütz, P.; Forrer, P.; Zahnd, C.; Plückthun, A. In vitro display technologies: novel developments and applications. Curr. Opin. Biotechnol., 2001, 12(4), 400-405.
6. He, M.; Taussig, M.J. Ribosome display of antibodies: expression, specificity and recovery in a eukaryotic system. J. Immunol Methods, 2005, 297(1-2), 73-82.
7. Binz, H.K.; Amstutz, P.; Kohl, A.; Stumpp, M.T.; Briand, C.; Forrer, P.; Grütter, M.G. Plückthun, A. High-affinity binders selected from designed ankyrin repeat protein libraries. Nat. Biotechnol., 2004, 22(5), 575-582.
8. He, M.; Taussig, M.J. Antibody-ribosome-mRNA (ARM) complexes as efficient selection particles for in vitro display and evolution of antibodies combining sites. Nucleic Acids Res., 1997, 25(24), 5132-5134.
9. Rothe, A.; Nathanielsz, A.; Hosse, R.J.; Oberhaüser, F.; Strandmann, E.P.; Engert, A.; Hudson, P.J.; Power, B.E. Selection of human anti-CD28 scFvs from a T-NHL related scFv library using ribosome display. J. Biotechnol, 2007, 130(4), 448-454.
10. Yan, X.; Xu, Z. Production of human single-chain variable fragment (scFv) antibody specific for digoxin by ribosome display. Indian J. Biochem. Biophys., 2005, 42(6), 350-357.
11. Fischer, C.; Jonckx, B.; Mazzone, M.; Zacchigna, S.; Loges, S.; Pattarini, L.; Chorianopoulos, E.; Liesenborghs, L.; Koch, M.; Mol, M. D.; Autiero, M.; Wyns, S.; Plaisance, S.; Moons, L.; v. Rooijen, N.; Giacca, M.; Stassen, J.M.; Dewerchin, M.; Collen, D.; Carmeliet, P. Anti-PlGF Inhibits Growth of VEGF(R)-inhibitorresistant tumors without affecting healthy vessels. Cell, 2007, 131(3), 463-475.
12. He, M.; Cooley, N.; Jackson, A.; Taussig, M.J. Production of human single-chain antibodies by ribosome display. Methods Mol. Biol., 2004, 248, 177-189.
13. Güssow, D.; Clackson, T. Direct clone characterization from plaques and colonies by the polymerase chain reaction. Nucleic Acids Res. 1989, 17(10), 4000.
14. Taussig, M.J.; Groves, M.A.; Menges, M.; Liu, H.; He, M. ARM complexes for in vitro display and evolution of antibody combining sites in Monoclonal Antibodies: A Practical Approach; Oxford Univ. Press: UK, 2000; pp. 91-109.
15. Coia, G.; Pontes-Braz, L.; Nuttall, S.D.; Hudson, P.J.; Irving, R.A. Panning and selection of proteins using ribosome display. J. Immunol. Methods, 2001, 254(1-2), 191-197.
16. Juraja, S.M.; Mulhern, T.D.; Hudson, P.J.; Hattarki, M.K.; Carmichael, J.A.; Nuttall, S.D. Engineering of the Escherichia coli Im7 immunity protein as a loop display scaffold. Protein Eng. Des. Sel., 2006, 19(5), 231-244.
17. Holliger, P.; Hudson, P.J. Engineered antibody fragments and the rise of single domains. Nat. Biotechnol., 2005, 23(9), 1126-1136.
18. He, M.; Taussig, M.J. Eukaryotic ribosome display with in situ DNA recovery. Nat. Methods, 2007, 4(3), 281-288.
19. Zahnd, C.; Amstutz, P.; Plückthun, A. Ribosome display: selecting and evolving proteins in vitro that specifically bind to a target. Nat. Methods, 2007, 4(3), 269-279.
20. Hanes, J.; Jermutus, L.; Schaffitzel, C.; Plückthun, A. Comparison of Escherichia coli and rabbit reticulocyte ribosome display systems. FEBS Lett., 1999, 450(1-2), 105-110.
21. Beatty, D. J.; Beatty, B. G.; Vlahos, W. G. Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay. J. Immunol. Methods, 1987, 100(1-2), 173-179.