Eukaryotic ribosome display with in situ DNA recovery

Nature Methods

Volumn 4, Issue 3, 2007, Pages 281-288

  He, Mingyue ^a   Taussig, Michael J ^a  

^a BABRAHAM INSTITUTE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; CELL EXTRACT; COMPLEMENTARY DNA; ENZYME; IMMUNOGLOBULIN; LIGAND; MESSENGER RNA; PEPTIDE; PROTEIN;

ARTICLE; BINDING AFFINITY; BINDING SITE; CELL FREE SYSTEM; COMPLEX FORMATION; DNA LIBRARY; EUKARYOTE; GENE AMPLIFICATION; GENE DELETION; GENOTYPE; IN VITRO SELECTION; MOLECULAR EVOLUTION; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROKARYOTE; PROTEIN EXPRESSION; PROTEIN ISOLATION; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RIBOSOME; RIBOSOME DISPLAY; RNA TRANSLATION; STOP CODON; TECHNOLOGY;

CELL-FREE SYSTEM; DIRECTED MOLECULAR EVOLUTION; DNA; POLYMERASE CHAIN REACTION; PROTEOME; RIBOSOMAL PROTEINS; RIBOSOMES;

EUKARYOTA; PROKARYOTA;

EID: 33847630761     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth1001     Document Type: Article

References (11)

1. He, M. & Taussig, M.J. ARM complexes as efficient selection particles for in vitro display and evolution of antibody combining sites. Nucleic Acids Res. 25, 5132-5134 (1997).
2. Hanes, J. & Plückthun, A. In vitro selection and evolution of functional proteins by using ribosome display. Proc. Natl. Acad. Sci. USA 94, 4937-4942 (1997).
3. He, M. & Khan, F. Ribosome display: Next-generation of display technologies for production of antibodies in vitro. Expert Rev. Proteomics 2, 421-430 (2005).
4. He, M. & Taussig, M.J. Ribosome display of antibodies: Expression, specificity and recovery in a eukaryotic system. J. Immunol. Methods. 297, 73-82 (2005).
5. He, M. et al. Selection of a human anti-progesterone antibody fragment from a transgenic mouse library by ARM ribosome display. J. Immunol. Methods 231, 105-117 (1999).
6. He, M, Cooley, N., Jackson, A. & Taussig, M. Production of human single-chain antibodies by ribosome display. Methods Mol. Biol. 248,177-189 (2004).
7. Douthwaite, J.A., Groves, M.A., Dufner, P. & Jermutus, L. An improved method for an efficient and easily accessible eukaryotic ribosome display technology. Protein Eng. Des. Sel. 19, 85-90 (2006).
8. Lipovsek, D. & Plückthun, A. In vitro protein evolution by ribosome display and mRNA display. J. Immunol. Methods 290, 51-67 (2004).
9. Zahnd, C., Amstutz, P. & Plückthun, A. Ribosome display selecting and evolving proteins in vitro that specifically bind to a target. Nat. Methods 4, 269-279 (2007).
10. Irving, R.A, Coia, G., Roberts, A., Nuttall, S.D. & Hudson, P.J. Ribosome display and affinity maturation: From antibodies to single V-domains and steps towards cancer therapeutics. J. Immunol. Methods 248, 31-45 (2001).
11. Rungpragayphan, S. et al. Cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression. J. Mol. Biol. 318, 395-405 (2002).