Purification and characterization of a viral chitinase active against plant pathogens and herbivores from transgenic tobacco

Journal of Biotechnology

Volumn 147, Issue 1, 2010, Pages 1-6

  Di Maro, Antimo ^a   Terracciano, Irma ^b   Sticco, Lucia ^a   Fiandra, Luisa ^c   Ruocco, Michelina ^b   Corrado, Giandomenico ^b   Parente, Augusto ^a   Rao, Rosa ^b  

^a SECOND UNIVERSITY OF NAPLES   (Italy)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^c UNIVERSITY OF MILAN   (Italy)

Author keywords

Biopesticides; Enzymatic activity; Nicotiana tabacum; Protein purification; Recombinant protein; Transgenic plant

Indexed keywords

BIOPESTICIDES; ENZYMATIC ACTIVITIES; NICOTIANA TABACUM; PROTEIN PURIFICATION; RECOMBINANT PROTEIN; TRANSGENIC PLANTS;

AMMONIUM COMPOUNDS; ANIMALS; ENZYMES; GELS; GENETICALLY MODIFIED PLANTS; INDUSTRIAL APPLICATIONS; PURIFICATION;

TOBACCO;

AMMONIUM SULFATE; AUTOGRAPHA CALIFORNICA NUCLEOPOLYHEDROVIRUS CHITINASE A; CHITINASE; UNCLASSIFIED DRUG; METHYLENE BLUE; RECOMBINANT PROTEIN;

ALTERNARIA ALTERNATA; ANION EXCHANGE; ARTICLE; BIOLOGICAL PEST CONTROL; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SYNTHESIS; FUNGAL SPORE GERMINATION; FUNGUS GROWTH; GEL FILTRATION CHROMATOGRAPHY; HELIOTHIS VIRESCENS; LARVA; LEPIDOPTERA; MEMBRANE PERMEABILITY; NONHUMAN; PLANT HERBIVORE INTERACTION; PRECIPITATION; PRIORITY JOURNAL; SERRATIA MARCESCENS; SILKWORM; TOBACCO; TRANSGENIC PLANT; ANIMAL; BACULOVIRIDAE; BIOSYNTHESIS; DRUG EFFECTS; ENZYMOLOGY; FEEDING BEHAVIOR; FUNGUS SPORE; GEL CHROMATOGRAPHY; GENETIC TRANSFORMATION; GENETICS; GERMINATION; INSECT; ISOLATION AND PURIFICATION; KINETICS; MEMBRANE; METABOLISM; MICROBIOLOGY; PH; PHYSIOLOGY; TEMPERATURE;

ANIMALS; CHITINASE; CHROMATOGRAPHY, GEL; ENZYME STABILITY; FEEDING BEHAVIOR; GERMINATION; HYDROGEN-ION CONCENTRATION; INSECTS; KINETICS; MEMBRANES; METHYLENE BLUE; NUCLEOPOLYHEDROVIRUS; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; SPORES, FUNGAL; TEMPERATURE; TOBACCO; TRANSFORMATION, GENETIC;

ALTERNARIA ALTERNATA; AUTOGRAPHA CALIFORNICA NUCLEOPOLYHEDROVIRUS; BOMBYX MORI; FUNGI; HELIOTHIS VIRESCENS; LEPIDOPTERA; NICOTIANA TABACUM; SERRATIA MARCESCENS;

EID: 77951975397     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.03.005     Document Type: Article

References (30)

1. Balasubramanian N., Juliet G.A.n.n.i.e., Srikalaivani P., Lalithakumari D. Release and regeneration of protoplasts from the fungus Trichothecium roseum. Can. J. Microbiol. 2003, 49:263-268.
2. Bhattacharya D., Nagpure A., Gupta R.K. Bacterial chitinases: properties and potential. Crit. Rev. Biotechnol. 2007, 27:21-28.
3. Byerlee D., de Janvry A., Sadoulet E. Agriculture for development: toward a new paradigm. Ann. Rev. Res. Econ. 2009, 1:15-31.
4. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
5. Claparols M.I., Bassie L., Miro B., Del Duca S., Rodriguez-Montesinos J., Christou P., Serafini-Fracassini D., Capell T. Transgenic rice as a vehicle for the production of the industrial enzyme transglutaminase. Trans. Res. 2004, 13:195-199.
6. Corrado G., Arciello A., Fanti P., Fiandra L., Garonna A., Digilio M.C., Lorito M., Giordana B., Pennacchio F., Rao R. The Chitinase A from the baculovirus AcMNPV enhances resistance to both fungi and herbivorous pests in tobacco. Trans. Res. 2008, 17:557-571.
7. Corrado G., Bovi P.D., Ciliento R., Gaudio L., Di Maro A., Aceto S., Lorito M., Rao R. Inducible expression of a phytolacca heterotepala ribosome-inactivating protein leads to enhanced resistance against major fungal pathogens in tobacco. Phytopathology 2005, 95:206-215.
8. de Wilde C., Uzan E., Zhou Z., Kruus K., Andberg M., Buchert J., Record E., Asther M., Lomascolo A.T. Transgenic rice as a novel production system for Melanocarpus and Pycnoporus laccases. Trans. Res. 2008, 17:515-527.
9. Di Maro A., Ferranti P., Mastronicola M., Polito L., Bolognesi A., Stirpe F., Malorni A., Parente A. Reliable sequence determination of ribosome-inactivating proteins by combining electrospray mass spectrometry and Edman degradation. J. Mass Spectrom. 2001, 36:38-46.
10. Dow J.A. pH gradients in Lepidopteran midgut. J. Exp. Biol. 1992, 172(1):355-375.
11. Ding X., Gopalakrishnan B., Johnson L., White F., Wang X., Morgan T.D., Kramer K.J., Muthukrishnan S. Insect resistance of transgenic tobacco expressing an insect chitinase gene. Trans. Res. 1998, 7:77-84.
12. Epstein L., Bassein S. Patterns of pesticide use in California and the implications for strategies for reduction of pesticides. Ann. Rev. Phytopathol. 2003, 41:351-375.
13. Hawtin R.E., Arnold K., Ayres M.D., Zanotto P.M., Howard S.C., Gooday G.W., Chappell L.H., Kitts P.A., King L.A., Possee R.D. Identification and preliminary characterization of a chitinase gene in the Autographa californica nuclear polyedrosis virus genome. Virology 1995, 212:673-685.
14. Howie W., Joe L., Newbigin E., Suslow T., Dunsmuir P. Transgenic tobacco plants which express the chiA gene from Serratia marcescens have enhanced tolerance to Rhizoctonia solani. Trans. Res. 1994, 3:90-98.
15. Hynes R.K., Boyetchko S.M. Research initiatives in the art and science of biopesticide formulations. Soil Biol. Biochem. 2006, 38:845-849.
16. Fiandra L., Casartelli M., Cermenati G., Burlini N., Giordana B. The intestinal barrier in lepidopteran larvae: permeability of the peritrophic membrane and of the midgut epithelium to two biologically active peptides. J. Insect Physiol. 2009, 55:10-18.
17. Kramer K.J., Koga D. Insect chitin: physical state, synthesis, degradation and metabolic regulation. Insect Biochem. 1986, 16:851-877.
18. Krokeide I.M., Synstad B., Gåseidnes S., Horn S.J., Eijsink V.G., Sørlie M. Natural substrate assay for chitinases using high-performance liquid chromatography: a comparison with existing assays. Anal. Biochem. 2007, 363:128-134.
19. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
20. Lineweaver H., Burk D. The determination of enzyme dissociation constants. J. Am. Chem. Soc. 1934, 56:658-666.
21. Lorito M., Hayes C.K., Di Pietro A., Woo S.L., Harman G.E. Purification, characterization and synergistic activity of a glucan 1,3-b-glucosidase and an N-acetyl-b-glucosaminidase from Trichoderma harzianum. Phytopathology 1994, 84:398-405.
22. McCreath K.J., Gooday G.W. A rapid and sensitive micro-assay for determination of chitinolytic activity. J. Microbiol. Methods 1992, 14:229-237.
23. Mizuno K., Kimura O., Tachiki T. Protoplast formation from Schizophyllum commune by a culture filtrate of Bacillus circulans KA-304 grown on a cell-wall preparation of S. commune as a carbon source. Biosci. Biotechnol. Biochem. 1997, 61:852-857.
24. Nikolov Z.L., Woodard S.L. Downstream processing of recombinant proteins from transgenic feedstock. Curr. Opin. Biotechnol. 2004, 15:479-486.
25. Patel M., Johnson J.S., Brettell R.I.S., Jacobsen J., Xue G.P. Transgenic barley expressing a fungal xylanase gene in the endosperm of the developing grains. Mol. Breed. 2000, 6:113-123.
26. Pichyangkura R., Kudan S., Kultiyawong K., Sukwattanasinitt M., Aiba S. Quantitative production of 2-acetamido-2-deoxy-d-glucose from crystalline chitin by bacterial chitinase. Carbohydr. Res. 2002, 337:557-559.
27. Rao R., Fiandra L., Giordana B., de Eguileor M., Congiu T., Burlini N., Arciello S., Corrado G., Pennacchio F. AcMNPV ChiA protein disrupts the peritrophic membrane and alters midgut physiology of Bombyx mori larvae. Insect Biochem. Mol. Biol. 2004, 34:1205-1213.
28. Twyman R.M., Stoger E., Schillberg S., Christou P., Fischer R. Molecular farming in plants: host systems and expression technology. Trends Biotechnol. 2003, 21:570-578.
29. Vyas P., Deshpande M.V. Enzymatic hydrolysis of chitin by Myrothecium verrucaria chitinase complex and its utilization to produce SCP. J. Gen. Appl. Microbiol. 1991, 37:265-275.
30. Ziegler M.T., Thomas S.R., Danna K.J. Accumulation of a thermostable endo-1,4-β-d-glucanase in the apoplast of Arabidopsis thaliana leaves. Mol. Breed. 2000, 6:37-46.