Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness

BMC Infectious Diseases

Volumn 10, Issue , 2010, Pages

  Szewczuk, Elektra ^a   Thapa, Kiran ^b   Anninos, Terry ^d   McPhie, Kenneth ^c   Higgins, Geoff ^d   Dwyer, Dominic E ^b,c   Stanley, Keith K ^a   Iredell, Jonathan R ^b,c  

^a AusDiagnostics Pty Ltd   (Australia)

^b UNIVERSITY OF SYDNEY   (Australia)

^c WESTMEAD HOSPITAL   (Australia)

^d INSTITUTE OF MEDICAL AND VETERINARY SCIENCE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

INFLUENZA VIRUS HEMAGGLUTININ; NUCLEOCAPSID PROTEIN; VIRUS NUCLEOPROTEIN; HEMAGGLUTININ FUSOGENIC PEPTIDE, INFLUENZA VIRUS; VIRUS HEMAGGLUTININ;

5' UNTRANSLATED REGION; ANTIGEN DETECTION; ARTICLE; CONTROLLED STUDY; DIFFERENTIAL DIAGNOSIS; FLU LIKE SYNDROME; HUMAN RHINOVIRUS; IMMUNOFLUORESCENCE; INFLUENZA A (H1N1); INFLUENZA A (H3N2); INFLUENZA A (H5N1); INFLUENZA B; INFLUENZA VIRUS A; INTERMETHOD COMPARISON; LABORATORY TEST; MULTIPLEX POLYMERASE CHAIN REACTION; NUCLEOTIDE SEQUENCE; PARAINFLUENZA VIRUS 3; QUANTITATIVE ANALYSIS; RESPIRATORY SYNCYTIAL VIRUS INFECTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIRUS IDENTIFICATION; VIRUS STRAIN; CLINICAL TRIAL; COMPARATIVE STUDY; EVALUATION; GENETICS; HUMAN; INFLUENZA; METHODOLOGY; MULTICENTER STUDY; POLYMERASE CHAIN REACTION; VIROLOGY;

DIAGNOSIS, DIFFERENTIAL; HEMAGGLUTININS, VIRAL; HUMANS; INFLUENZA A VIRUS; INFLUENZA, HUMAN; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIROLOGY;

EID: 77951942857     PISSN: None     EISSN: 14712334     Source Type: Journal    
DOI: 10.1186/1471-2334-10-113     Document Type: Article

References (36)

1. Nicholson KG. Impact of influenza and respiratory syncytial virus on mortality in England and Wales from January 1975 to December 1990. Epidemiol Infect 1996, 116:51-63. 10.1017/S0950268800058957, 2271234, 8626004.
2. Sander B, Nizam A, Garrison LP, Postma MJ, Halloran ME, Longini IM. Economic Evaluation of Influenza Pandemic Mitigation Strategies in the United States Using a Stochastic Microsimulation Transmission Model. Value Health 2008,
3. Hien TT, de Jong M, Farrar J. Avian influenza--a challenge to global health care structures. N Engl J Med 2004, 351:2363-2365. 10.1056/NEJMp048267, 15575048.
4. Pabbaraju K, Tokaryk KL, Wong S, Fox JD. Comparison of the Luminex xTAG respiratory viral panel with in-house nucleic acid amplification tests for diagnosis of respiratory virus infections. J Clin Microbiol 2008, 46:3056-3062. 10.1128/JCM.00878-08, 2546709, 18632902.
5. Playford EG, Dwyer DE. Laboratory diagnosis of influenza virus infection. Pathology 2002, 34:115-125. 10.1080/003130201201117909, 12009091.
6. Hurt AC, Alexander R, Hibbert J, Deed N, Barr IG. Performance of six influenza rapid tests in detecting human influenza in clinical specimens. J Clin Virol 2007, 39:132-135. 10.1016/j.jcv.2007.03.002, 17452000.
7. Mehlmann M, Bonner AB, Williams JV, Dankbar DM, Moore CL, Kuchta RD, Podsiad AB, Tamerius JD, Dawson ED, Rowlen KL. Comparison of the MChip to viral culture, reverse transcription-PCR, and the QuickVue influenza A+B test for rapid diagnosis of influenza. J Clin Microbiol 2007, 45:1234-1237. 10.1128/JCM.02202-06, 1865827, 17301287.
8. Smit M, Beynon KA, Murdoch DR, Jennings LC. Comparison of the NOW Influenza A & B, NOW Flu A, NOW Flu B, and Directigen Flu A+B assays, and immunofluorescence with viral culture for the detection of influenza A and B viruses. Diagn Microbiol Infect Dis 2007, 57:67-70. 10.1016/j.diagmicrobio.2006.11.003, 17178298.
9. Blyth CC, Iredell JR, Dwyer DE. Rapid-Test Sensitivity for Novel Swine-Origin Influenza A (H1N1) Virus in Humans. N Engl J Med 2009, 361:1925. 10.1056/NEJMc0909049, 19815860.
10. Kok J, Blyth CC, Foo H, Patterson J, Taylor J, McPhie K, Ratnamohan VM, Iredell JR, Dwyer DE. Comparison of a Rapid Antigen Test with Nucleic Acid Testing During Co-circulation of Pandemic Influenza A/H1N1 2009 and Seasonal Influenza A/H3N2. J Clin Microbiol 2009, 48:290-291. 10.1128/JCM.01465-09, 19889892.
11. Stanley KK, Szewczuk E. Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection. Nucleic Acids Res 2005, 33:e180. 10.1093/nar/gni182, 1298933, 16314310.
12. Stelzer-Braid S, Escott R, Baleriola C, Kirkland P, Robertson P, Catton M, Rawlinson WD. Proficiency of nucleic acid tests for avian influenza viruses, Australasia. Emerg Infect Dis 2008, 14:1126-1128. 10.3201/eid1407.071098, 2600363, 18598638.
13. Foo H, Blyth CC, van Hal S, McPhie K, Ratnamohan M, Fennell M, Ba Alawi F, Rawlinson W, Adamson S, Armstrong P, Dwyer DE. Laboratory test performance in young adults during influenza outbreaks at World Youth Day 2008. J Clin Virol 2009, 46:384-386. 10.1016/j.jcv.2009.09.019, 19828366.
14. Rozen S, Skaletsky HJ. Primer3 on the www for general users and for biologist programmers. Bioinformatics Methods and Protocols: Methods in Molecular Biology 2000, 365-386. Totowa, NJ: Humana Press, Krawetz S, Misener S.
15. Druce J, Tran T, Kelly H, Kaye M, Chibo D, Kostecki R, Amiri A, Catton M, Birch C. Laboratory diagnosis and surveillance of human respiratory viruses by PCR in Victoria, Australia, 2002-2003. J Med Virol 2005, 75:122-129. 10.1002/jmv.20246, 15543580.
16. Stone B, Burrows J, Schepetiuk S, Higgins G, Hampson A, Shaw R, Kok T. Rapid detection and simultaneous subtype differentiation of influenza A viruses by real time PCR. J Virol Methods 2004, 117:103-112. 10.1016/j.jviromet.2003.12.005, 15041206.
17. Hoffmann E, Stech J, Guan Y, Webster RG, Perez DR. Universal primer set for the full-length amplification of all influenza A viruses. Archives of Virology 2001, 146:2275-2289. 10.1007/s007050170002, 11811679.
18. Kong F, Gordon S, Gilbert GL. Rapid-Cycle PCR for Detection and Typing of Mycoplasma pneumoniae in Clinical Specimens. J Clin Microbiol 2000, 38:4256-4259. 87576, 11060103.
19. McDonough EA, Barrozo CP, Russell KL, Metzgar D. A multiplex PCR for detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in clinical specimens. Molecular and Cellular Probes 2005, 19:314-322. 10.1016/j.mcp.2005.05.002, 16024220.
20. Stone B, Burrows J, Schepetiuk S, Higgins G, Hampson A, Shaw R, Kok T. Rapid detection and simultaneous subtype differentiation of influenza A viruses by real time PCR. J Virol Methods 2004, 117:103-112. 10.1016/j.jviromet.2003.12.005, 15041206.
21. Thawatsupha P, Waicharoen S, Maneewong P, Prasittikhet K, Chittaganapitch M, Sawanpanyalert P. Isolation and identification of influenza virus strains circulating in Thailand in 2001. Southeast Asian J Trop Med Public Health 2003, 34:94-97.
22. Jackson LA, Cherry JD, Wang SP, Grayston JT. Frequency of serological evidence of Bordetella infections and mixed infections with other respiratory pathogens in university students with cough illnesses. Clin Infect Dis 2000, 31:3-6. 10.1086/313911, 10913388.
23. Yuen KY, Wong SS. Human infection by avian influenza A H5N1. Hong Kong Med J 2005, 11:189-199.
24. Ng EK, Cheng PK, Ng AY, Hoang TL, Lim WW. Influenza A H5N1 detection. Emerg Infect Dis 2005, 11:1303-1305.
25. Moore C, Hibbitts S, Owen N, Corden SA, Harrison G, Fox J, Gelder C, Westmoreland D. Development and evaluation of a real-time nucleic acid sequence based amplification assay for rapid detection of influenza A. J Med Virol 2004, 74:619-628. 10.1002/jmv.20221, 15484279.
26. Whiley DM, Sloots TP. A 5'-nuclease real-time reverse transcriptase-polymerase chain reaction assay for the detection of a broad range of influenza A subtypes, including H5N1. Diagn Microbiol Infect Dis 2005,
27. Li PQ, Yang ZF, Chen JX, Muller CP, Zhang J, Wang DF, Zhang R, He YS. Simultaneous detection of different respiratory virus by a multiplex reverse transcription polymerase chain reaction combined with flow-through reverse dot blotting assay. Diagn Microbiol Infect Dis 2008, 62:44-51. 10.1016/j.diagmicrobio.2008.04.017, 18639996.
28. Kong F, Gilbert GL. Multiplex PCR-based reverse line blot hybridization assay (mPCR/RLB)--a practical epidemiological and diagnostic tool. Nat Protoc 2006, 1:2668-2680. 10.1038/nprot.2006.404, 17406523.
29. Lam WY, Yeung AC, Tang JW, Ip M, Chan EW, Hui M, Chan PK. Rapid multiplex nested PCR for detection of respiratory viruses. J Clin Microbiol 2007, 45:3631-3640. 10.1128/JCM.00280-07, 2168518, 17804659.
30. Legoff J, Kara R, Moulin F, Si-Mohamed A, Krivine A, Belec L, Lebon P. Evaluation of the one-step multiplex real-time reverse transcription-PCR ProFlu-1 assay for detection of influenza A and influenza B viruses and respiratory syncytial viruses in children. J Clin Microbiol 2008, 46:789-791. 10.1128/JCM.00959-07, 2238076, 18057126.
31. Beigel JH, Farrar J, Han AM, Hayden FG, Hyer R, de Jong MD, Lochindarat S, Nguyen TK, Nguyen TH, Tran TH, et al. Avian influenza A (H5N1) infection in humans. N Engl J Med 2005, 353:1374-1385. 10.1056/NEJMra052211, 16192482.
32. de Jong MD, Hien TT. Avian influenza A (H5N1). J Clin Virol 2005,
33. Webb SA, Pettilä V, Seppelt I, Bellomo R, Bailey M, Cooper DJ, Cretikos M, Davies AR, Finfer S, Harrigan PW, Hart GK, Howe B, Iredell JR, McArthur C, Mitchell I, Morrison S, Nichol AD, Paterson DL, Peake S, Richards B, Stephens D, Turner A, Yung M. ANZIC Influenza Investigators Critical care services and 2009 H1N1 influenza in Australia and New Zealand. New Engl J Med 2009, 361:1925-34. 10.1056/NEJMoa0908481, 19815860, ANZIC Influenza Investigators.
34. Bradt DA, Drummond CM. Avian influenza pandemic threat and health systems response. Emerg Med Australas 2006, 18:430-443. 10.1111/j.1742-6723.2006.00906.x, 17083631.
35. Ellis SE, Coffey CS, Mitchel EF, Dittus RS, Griffin MR. Influenza- and respiratory syncytial virus-associated morbidity and mortality in the nursing home population. J Am Geriatr Soc 2003, 51:761-767. 10.1046/j.1365-2389.2003.51254.x, 12757561.
36. Thompson WW, Shay DK, Weintraub E, Brammer L, Cox N, Anderson LJ, Fukuda K. Mortality associated with influenza and respiratory syncytial virus in the United States. JAMA 2003, 289:179-186. 10.1001/jama.289.2.179, 12517228.