Simultaneous detection of different respiratory virus by a multiplex reverse transcription polymerase chain reaction combined with flow-through reverse dot blotting assay

Diagnostic Microbiology and Infectious Disease

Volumn 62, Issue 1, 2008, Pages 44-51

  Li, Pei qiong ^a   Yang, Zi feng ^b,c   Chen, Jing xian ^c   Muller, Claude P ^d   Zhang, Jun ^e   Wang, Dan fen ^c   Zhang, Ren ^f   He, Yun shao ^e  

^a GUANGZHOU MEDICAL UNIVERSITY   (China)

^b MACAU UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Macao)

^c GUANGZHOU MEDICAL UNIVERSITY   (China)

^d National Public Health Laboratory   (Belgium)

^e SUN YAT SEN UNIVERSITY   (China)

^f GUANGZHOU UNIVERSITY OF CHINESE MEDICINE   (China)

Author keywords

Diagnostic method; Flow through reverse dot blotting; Multiplex reverse transcription polymerase chain reaction; Respiratory virus

Indexed keywords

ADULT; ARTICLE; CONTROLLED STUDY; COXSACKIE VIRUS; FEMALE; HUMAN; HUMAN RHINOVIRUS; IMMUNOFLUORESCENCE TEST; INFLUENZA VIRUS A; INFLUENZA VIRUS B; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALE; NONHUMAN; PARAINFLUENZA VIRUS 1; PARAINFLUENZA VIRUS 3; PRIORITY JOURNAL; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; RESPIRATORY VIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRAL RESPIRATORY TRACT INFECTION; VIRUS CULTURE; VIRUS DETECTION; VIRUS STRAIN;

EID: 49549108676     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2008.04.017     Document Type: Article

References (29)

1. Aguilar J.C., Perez-Brena M.P., Garcia M.L., Cruz N., Erdman D.D., and Echevarria J.E. Detection and identification of human parainfluenza viruses 1, 2, 3, and 4 in clinical samples of pediatric patients by multiplex reverse transcription-PCR. J. Clin. Microbiol. 38 (2000) 1191-1195
2. Atmar R.L., Baxter B.D., Dominguez E.A., and Taber L.H. Comparison of reverse transcription-PCR with tissue culture and other rapid diagnostic assays for detection of type A influenza virus. J. Clin. Microbiol. 34 (1996) 2604-2606
3. Bellau-Pujol S., Vabret A., Legrand L., Dina J., Gouarin S., Petitjean-Lecherbonnier J., Pozzetto B., Ginevra C., and Freymuth F. Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses. J. Virol. Methods 126 (2005) 53-63
4. Boivin G., Cote S., Dery P., De Serres G., and Bergeron M.G. Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses. J. Clin. Microbiol. 42 (2004) 45-51
5. Cate T.R., Rossen R.D., Douglas Jr. R.G., Butler W.T., and Couch R.B. The role of nasal secretion and serum antibody in the rhinovirus common cold. Am. J. Epidemiol. 84 (1966) 352-363
6. Coiras M.T., Perez-Brena P., Garcia M.L., and Casas I. Simultaneous detection of influenza A, B, and C viruses, respiratory syncytial virus, and adenoviruses in clinical samples by multiplex reverse transcription nested-PCR assay. J. Med. Virol. 69 (2003) 132-144
7. Coiras M.T., Lopez-Huertas M.R., Lopez-Campos G., Aguilar J.C., and Perez-Brena P. Oligonucleotide array for simultaneous detection of respiratory viruses using a reverse-line blot hybridization assay. J. Med. Virol. 76 (2005) 256-264
8. Corne J.M., Green S., Sanderson G., Caul E.O., and Johnston S.L. A multiplex RT-PCR for the detection of parainfluenza viruses 1-3 in clinical samples. J. Virol. Methods 82 (1999) 9-18
9. Elnifro E.M., Ashshi A.M., Cooper R.J., and Klapper P.E. Multiplex PCR: optimization and application in diagnostic virology. Clin. Microbiol. Rev. 13 (2000) 559-570
10. Fan J., Henrickson K.J., and Savatski L.L. Rapid simultaneous diagnosis of infections with respiratory syncytial viruses A and B, influenza viruses A and B, and human parainfluenza virus types 1, 2, and 3 by multiplex quantitative reverse transcription-polymerase chain reaction-enzyme hybridization assay (Hexaplex). Clin. Infect. Dis. 26 (1998) 1397-1402
11. Gunson R.N., Collins T.C., and Carman W.F. Real-time RT-PCR detection of 12 respiratory viral infections in four triplex reactions. J. Clin. Virol. 33 (2005) 341-344
12. Lin B., Blaney K.M., Malanoski A.P., Ligler A.G., Schnur J.M., Metzgar D., Russell K.L., and Stenger D.A. Using a resequencing microarray as a multiple respiratory pathogen detection assay. J. Clin. Microbiol. 45 (2007) 443-452
13. Liolios L., Jenney A., Spelman D., Kotsimbos T., Catton M., and Wesselingh S. Comparison of a multiplex reverse transcription-PCR-enzyme hybridization assay with conventional viral culture and immunofluorescence techniques for the detection of seven viral respiratory pathogens. J. Clin. Microbiol. 39 (2001) 2779-2783
14. Magnard C., Valette M., Aymard M., and Lina B. Comparison of two nested PCR, cell culture, and antigen detection for the diagnosis of upper respiratory tract infections due to influenza viruses. J. Med. Virol. 59 (1999) 215-220
15. Markoulatos P., Siafakas N., and Moncany M. Multiplex polymerase chain reaction: a practical approach. J. Clin. Lab. Anal. 16 (2002) 47-51
16. McLaws M.L., Murphy C., Taylor P., Ferson M., Gold J., Donnellan R., and Dalton D. Rate of seasonal spread of respiratory syncytial virus in a pediatric hospital. Infect. Control Hosp. Epidemiol. 18 (1997) 778-780
17. Osiowy C. Direct detection of respiratory syncytial virus, parainfluenza virus, and adenovirus in clinical respiratory specimens by a multiplex reverse transcription-PCR assay. J. Clin. Microbiol. 36 (1998) 3149-3154
18. Ou Z.Y., Liu N., Chen C.J., Cheng G., and He Y.S. Rapid and accurate genotyping of YMDD motif variants in the hepatitis B virus genome by an improved reverse dot blot method. J. Clin. Microbiol. 43 (2005) 5685-5689
19. Payungporn S., Chutinimitkul S., Chaisingh A., Damrongwantanapokin S., Buranathai C., Amonsin A., Theamboonlers A., and Poovorawan Y. Single step multiplex real-time RT-PCR for H5N1 influenza A virus detection. J. Virol. Methods 131 (2006) 143-147
20. Perdue M.L., and Swayne D.E. Public health risk from avian influenza viruses. Avian Dis. 49 (2005) 317-327
21. Poddar S.K. Influenza virus types and subtypes detection by single step single tube multiplex reverse transcription-polymerase chain reaction (RT-PCR) and agarose gel electrophoresis. J. Virol. Methods 99 (2002) 63-70
22. Syrmis M.W., Whiley D.M., Thomas M., Mackay I.M., Williamson J., Siebert D.J., Nissen M.D., and Sloots T.P. A sensitive, specific, and cost-effective multiplex reverse transcriptase-PCR assay for the detection of seven common respiratory viruses in respiratory samples. J. Mol. Diagn. 6 (2004) 125-131
23. Templeton K.E., Scheltinga S.A., Beersma M.F., Kroes A.C., and Claas E.C. Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza a and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4. J. Clin. Microbiol. 42 (2004) 1564-1569
24. Trampuz A., Prabhu R.M., Smith T.F., and Baddour L.M. Avian influenza: a new pandemic threat?. Mayo Clin. Proc. 79 (2004) 523-530 (quiz 530)
25. van Elden L.J., van Kraaij M.G., Nijhuis M., Hendriksen K.A., Dekker A.W., Rozenberg-Arska M., and van Loon A.M. Polymerase chain reaction is more sensitive than viral culture and antigen testing for the detection of respiratory viruses in adults with hematological cancer and pneumonia. Clin. Infect. Dis. 34 (2002) 177-183
26. van Milaan A.J., Sprenger M.J., Rothbarth P.H., Brandenburg A.H., Masurel N., and Claas E.C. Detection of respiratory syncytial virus by RNA-polymerase chain reaction and differentiation of subgroups with oligonucleotide probes. J. Med. Virol. 44 (1994) 80-87
27. Weigl J.A., Puppe W., Grondahl B., and Schmitt H.J. Epidemiological investigation of nine respiratory pathogens in hospitalized children in Germany using multiplex reverse-transcriptase polymerase chain reaction. Eur. J. Clin. Microbiol. Infect. Dis. 19 (2000) 336-343
28. Zavattoni M., Percivalle E., Cattaneo E., Revello M.G., Torsellini M., and Gerna G. Optimized detection of respiratory viruses in nasopharyngeal secretions. New Microbiol. 26 (2003) 133-140
29. Zhang R., Deng Y., Muller C.P., Ou Z.Y., Ma L., Wang M., Li P.Q., and He Y.S. Determination of hepatitis B virus genotype by flow-through reverse dot blot. J. Clin. Virol. 39 (2007) 94-100