Inhibition of latent transforming growth factor-b1 activation by lentivirus-mediated short hairpin RNA targeting the CD36 gene in NR8383 cells

Molecular Biology Reports

Volumn 37, Issue 3, 2010, Pages 1649-1655

  Wang, Xin ^a   Chen, Ying ^a   Lv, Lina ^a   Chen, Jie ^a  

^a CHINA MEDICAL UNIVERSITY   (China)

Author keywords

CD36; Latent transforming growth factor b1; Lentiviral vector; NR8383 cells; Short hairpin RNA

Indexed keywords

BLEOMYCIN; CD36 ANTIGEN; LENTIVIRUS VECTOR; MESSENGER RNA; SHORT HAIRPIN RNA; TRANSFORMING GROWTH FACTOR BETA1; OLIGONUCLEOTIDE; RNA; TGFB1 PROTEIN, RAT;

ANIMAL CELL; ARTICLE; CELL LINE; CELL STIMULATION; CELL STRAIN HEK293; CELL STRAIN NR8383; CONTROLLED STUDY; GENE EXPRESSION; GENE TARGETING; LUNG ALVEOLUS MACROPHAGE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN SECRETION; RAT; REAL TIME POLYMERASE CHAIN REACTION; WESTERN BLOTTING; ANALYSIS OF VARIANCE; ANIMAL; GENE VECTOR; GENETICS; INVERTED REPEAT; LENTIVIRINAE; METABOLISM; METHODOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

LENTIVIRUS; RATTUS;

ANALYSIS OF VARIANCE; ANIMALS; ANTIGENS, CD36; BLEOMYCIN; BLOTTING, WESTERN; CELL LINE; GENE TARGETING; GENETIC VECTORS; INVERTED REPEAT SEQUENCES; LENTIVIRUS; OLIGONUCLEOTIDES; RATS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; TRANSFORMING GROWTH FACTOR BETA1;

EID: 77951917889     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-009-9579-2     Document Type: Article

References (23)

1. Khalil N, Whitman C, Zuo L, Danielpour D, Greenberg A (1993) Regulation of alveolar macrophage transforming growth factorbeta secretion by corticosteroids in bleomycin induced pulmonary inflammation in the rat. J Clin Invest 92:1812-1818. doi: 10.1172/JCI116771
2. Cutroneo KR, White SL, Phan SH, Ehrlich HP (2007) Therapies for bleomycin induced lung fibrosis through regulation of TGFbeta1 induced collagen gene expression. J Cell Physiol 211:585-589. doi:10.1002/jcp.20972
3. Chen Y, Chen J, Dong J, Liu W (2005) Antifibrotic effect of interferon gamma in silicosis model of rat. Toxicol Lett 155:353-360. doi:10.1016/j.toxlet. 2004.10.011
4. Daniels CE, Wilkes MC, Edens M, Kottom TJ, Murphy SJ, Limper AH, Leof EB (2004) Imatinib mesylate inhibits the profibrogenic activity of TGF-β and prevents bleomycin mediated lung fibrosis. J Clin Invest 114:1308-1316
5. Yehualaeshet T, O'Connor R, Green-Johnson J, Mai S, Silverstein R, Murphy-Ullrich JE, Khalil N (1999) Activation of rat alveolar macrophage-derived latent transforming growth factorb1 by plasmin requires interaction with thrombospondin-1 and its cell surface receptor, CD36. Am J Pathol 155:841-851
6. Yang YL, Lin SH, Chuang LY, Guh JY, Liao TN, Lee TC, Chang WT, Chang FR, Hung MY, Chiang TA, Hung CY (2007) CD36 is a novel and potential anti-fibrogenic target in albumin-induced renal proximal tubule fibrosis. J Cell Biochem 101:735-744. doi: 10.1002/jcb.21236
7. Yehualaeshet T, O'Connor R, Begleiter A, Murphy-Ullrich JE, Silverstein R, Khalil N (2000) A CD36 synthetic peptide inhibits bleomycin-induced pulmonary inflammation and connective tissue synthesis in the rat. Am J Respir Cell Mol Biol 23:204-212
8. Gartel AL, Kandel ES (2006) RNA interference in cancer. Biomol Eng 23:17-34. doi:10.1016/j.bioeng.2006.01.002
9. Lu PY, Xie F, Woodle MC (2005) In vivo application of RNA interference: From functional genomics to therapeutics. Adv Genet 54:117-142
10. Abbas-Terki T, Blanco-Bose W, Déglon N, Pralong W, Aebischer P (2002) Lentiviral-mediated RNA interference. Hum Gene Ther 13:2197-2201. doi:10.1089/104303402320987888
11. Li M, Rossi JJ (2008) Lentiviral vector delivery of siRNA and shRNA encoding genes into cultured and primary hematopoietic cells. Methods Mol Biol 433:287-299. doi:10.1007/978-1-59745- 237-3-18
12. Zielske SP, Stevenson M (2005) Importin 7 may be dispensable for human immunodeficiency virus type 1 and simian immunodeficiency virus infection of primary macrophages. J Virol 79: 11541-11546. doi:10.1128/JVI.79.17.11541-11546. 2005
13. Pullmann R Jr, Juhaszova M, López de Silanes I, Kawai T, Mazan-Mamczarz K, Halushka MK, Gorospe M (2005) Enhanced proliferation of cultured human vascular smooth muscle cells linked to increased function of RNA-binding protein HuR. J Biol Chem 280:22819-22826. doi:10.1074/jbc.M501106 200
14. Sakoda T, Kasahara N, Hamamori Y, Kedes L (1999) A high-titer lentiviral production system mediates efficient transduction of differentiated cells including beating cardiac myocytes. J Mol Cell Cardiol 31:2037-2047. doi:10.1006/jmcc.1999.1035
15. Soneoka Y, Cannon PM, Ramsdale EE, Griffiths JC, Romano G, Kingsman SM, Kingsman AJ (1995) A transient three-plasmid expression system for the production of high titer retroviral vectors. Nucleic Acids Res 23:628-633. doi:10.1093/nar/23.4.628
16. Denholm EM, Rollins SM (1993) Expression and secretion of transforming growth factor-beta by bleomycin-stimulated rat alveolar macrophages. Am J Physiol Lung Cell Mol Physiol 264:L36-L42
17. Xu Y, Mirmalek-Sani SH, Lin F, Zhang J, Oreffo RO (2007) Adipocyte differentiation induced using nonspecific siRNA controls in cultured human mesenchymal stem cells. RNA 13:1179-1183. doi:10.1261/rna.527207
18. Febbraio M, Hajjar DP, Silverstein RL (2001) CD36: A class B scavenger receptor involved in angiogenesis, atherosclerosis, inflammation, and lipid metabolism. J Clin Invest 108:785-791
19. Pfeifer A, Eigenbrod S, Al-Khadra S, Hofmann A, Mitteregger G, Moser M, Bertsch U, Kretzschmar H (2006) Lentivector-mediated RNAi efficiently suppresses prion protein and prolongs survival of scrapie-infected mice. J Clin Invest 116:3204-3210. doi:10.1172/JCI29236
20. Liu L, Zhang Q, Zhang Y, Wang S, Ding Y (2006) Lentivirusmediated silencing of Tiam1 gene influences multiple functions of a human colorectal cancer cell line. Neoplasia 8:917-924. doi: 10.1593/neo.06364
21. Katayama K, Wada K, Miyoshi H, Ohashi K, Tachibana M, Furuki R, Mizuguchi H, Hayakawa T, Nakajima A, Kadowaki T, Tsutsumi Y, Nakagawa S, Kamisaki Y, Mayumi T (2004) RNA interfering approach for clarifying the PPARgamma pathway using lentiviral vector expressing short hairpin RNA. FEBS Lett 560:178-182. doi:10.1016/S0014-5793(04)00100-0
22. Kuchibhotla S, Vanegas D, Kennedy DJ, Guy E, Nimako G, Morton RE, Febbraio M (2008) Absence of CD36 protects against atherosclerosis in ApoE knock-out mice with no additional protection provided by absence of scavenger receptor A I/II. Cardiovasc Res 78:185-196. doi:10.1093/cvr/cvm093
23. Khalil N (2001) Post translational activation of latent transforming growth factor beta (L-TGF-βeta): Clinical implications. Histol Histopathol 16:541-551