Synthesis of heterocycle-linked thioureas and their inhibitory activities of NO production in LPS activated macrophages

Bulletin of the Korean Chemical Society

Volumn 31, Issue 1, 2010, Pages 27-30

  Cheon, Ye Jin ^a   Gim, Hyo Jin ^a   Jang, Hee Ryun ^a   Ryu, Jae Ha ^a   Jeon, Raok ^a  

^a Sookmyung Women's University   (South Korea)

Author keywords

INOS; Nitric oxide; Thiourea

Indexed keywords

ACTIVATED MACROPHAGES; BENEFICIAL EFFECTS; HETEROCYCLES; INHIBITORY ACTIVITY; INOS; LIPOPOLYSACCHARIDES; MRNA EXPRESSION; RT-PCR ANALYSIS;

MACROPHAGES; NITRIC OXIDE; SYNTHESIS (CHEMICAL); THIOUREAS;

UREA;

EID: 77951865642     PISSN: 02532964     EISSN: 12295949     Source Type: Journal    
DOI: 10.5012/bkcs.2010.31.01.027     Document Type: Article

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21. 50 values.
22. 6 cells/60 mm dish) were stimulated for 6 h with LPS (1 μg/mL) in the absence or presence of test samples. Total RNA was isolated from cell pellet using an RNA isolation reagent (Trizol, Invitrogen, Carlsbad, CA). Two microgram of RNA was reverse transcribed into cDNA using reverse transcriptase (Invitrogen, Carlsbad, CA) and random hexamer (Cosmo, Seoul, Korea). The PCR samples, contained in the reaction mixture, were comprised of mixture buffer, dNTP, Taq DNA polymerase (Promega, Madison, WI) and primers (sense and antisense). The sense and antisense primers for iNOS were 5'-CCCTTCCGAAGTTTCTGGCAGCAG-3' and 5'-GGCTGTCAGAGCCTCGTGGCTTTGG-3', respectively. The sesnse and antisense primers for β-actin were 5'-TGTGATGGT GGGAATGGGTCAG-3' and 5'-TTTGATGTCACGCACGATT TCC-3', respectively. The PCR amplification was performed under following conditions; 25 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s and extension at 72 °C for 30 s, using thermal cycler (Gene Amp PCR system 2400, Applied Biosystems, Foster City, CA). The amplified PCR products were separated on a 1% agarose gel.