Synthesis of phenylisothiourea derivatives as inhibitors of NO production in LPS activated macrophages

Bioorganic and Medicinal Chemistry Letters

Volumn 19, Issue 11, 2009, Pages 3088-3092

  Jin, Guo Hua ^a   Lee, Da Yeon ^a   Cheon, Ye Jin ^a   Gim, Hyo Jin ^a   Kim, Do Hee ^a   Kim, Hee Doo ^a   Ryu, Jae Ha ^a   Jeon, Raok ^a  

^a Sookmyung Women's University   (South Korea)

Author keywords

iNOS; Isothiourea; Nitric oxide

Indexed keywords

CARBAZOLE DERIVATIVE; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INDUCIBLE NITRIC OXIDE SYNTHASE; LIPOPOLYSACCHARIDE; MESSENGER RNA; NITRIC OXIDE; P65 PROTEIN; PHENYLISOTHIOUREA DERIVATIVE; PROTEIN; THIOUREA DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; INHIBITION KINETICS; LIPOPHILICITY; MACROPHAGE ACTIVATION; SYNTHESIS;

LIPOPOLYSACCHARIDES; MACROPHAGES; NF-KAPPA B; NITRIC OXIDE; NITRIC OXIDE SYNTHASE TYPE II; THIOUREA;

EID: 65149085044     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2009.04.001     Document Type: Article

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25. 6 cells/60 mm dish) were stimulated with LPS (1 μg/mL) in the presence or absence of test compounds. After incubation for 20 h, the cells were washed and lyzed with lysis buffer. Twenty micrograms of protein of cell lysates were applied on 8% SDS-polyacrylamide gels and transferred to PVDF membrane by a standard method. The membrane was probed with antibody for anti-mouse iNOS (Transduction Laboratories, Lexington, KY) and anti-actin (Sigma, St. Louis, MO). The bands were visualized using an enhanced chemiluminescence (ECL) detection kit (Amersham Bioscience, Piscataway, NJ) according to the manufacturer's instruction.
26. 6 cells/60 mm dish) were stimulated for 6 h with LPS (1 μg/mL) in the presence or absence of test compounds. After washing twice with phosphate buffered saline, total RNA was isolated from cell pellet, using an RNA isolation reagent (Trizol, Invitrogen, Carlsbad, CA). Two microgram of RNA was reverse transcribed into cDNA using reverse transcriptase and random hexamer. The PCR samples, contained in the reaction mixture, were comprised of mixture buffer, dNTP, Taq DNA polymerase (Promega, Madison, WI) and primers (sense and antisense). The sense and antisense primers for iNOS were 5′-ATGTCCGAAGCAAACATCAC-3′ and 5′-TAATGTCCAGGAAGTAGGTG-3′, respectively. The sense and antisense primers for β-actin were 5′-TGTGATGGTGGGAATGGGTCAG-3′ and 5′-TTTGATGTCACGCACGATTTCC-3′, respectively. The PCR amplification was performed under following conditions; 25 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s and extension at 72 °C for 30 s, using thermal cycler (Gene Amp PCR system 2400, Applied Biosystems, Foster City, CA). The amplified PCR products were separated on a 2% agarose gel.
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