Parameters affecting the separation of intact proteins in gradient-elution reversed-phase chromatography using poly(styrene-co-divinylbenzene) monolithic capillary columns

Journal of Chromatography A

Volumn 1217, Issue 18, 2010, Pages 3085-3090

  Detobel, Frederik ^a   Broeckhoven, Ken ^a   Wellens, Joke ^a   Wouters, Bert ^a   Swart, Remco ^b   Ursem, Mario ^b   Desmet, Gert ^a   Eeltink, Sebastiaan ^a  

^a VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

^b Dionex Corporation   (Netherlands)

Author keywords

Column technology; High performance liquid chromatography; Proteomics

Indexed keywords

ANALYSIS TIME; COLUMN LENGTHS; COLUMN TECHNOLOGY; CONSTANT GRADIENTS; EXPERIMENTAL STUDIES; GRADIENT TIME; HOLDUP TIME; INTACT PROTEINS; MACROPORE SIZE; MONOLITHIC CAPILLARY COLUMN; MONOLITHIC COLUMNS; PEAK CAPACITY; PEAK WIDTHS; PER UNIT; POLY(STYRENE-CO-DIVINYLBENZENE); POLYMER MONOLITHS; POROUS SILICA; PROTEOMICS; REVERSED-PHASE CHROMATOGRAPHY; SQUARE ROOTS; TIME FRAME;

LIQUID CHROMATOGRAPHY; LIQUIDS; MOLECULAR BIOLOGY; PROTEINS; SILICA; STYRENE;

COLUMN CHROMATOGRAPHY;

COPOLYMER; POLY(STYRENE CO DIVINYLBENZENE); PROTEIN; SILICON DIOXIDE; UNCLASSIFIED DRUG;

ARTICLE; CAPILLARY CHROMATOGRAPHY; CONTROLLED STUDY; ELUTION; EXPERIMENTAL STUDY; FLOW RATE; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEOMICS; REVERSED PHASE LIQUID CHROMATOGRAPHY; SEPARATION TECHNIQUE; TEMPERATURE SENSITIVITY;

ANIMALS; CATTLE; CHICKENS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; HORSES; POLYSTYRENES; PROTEINS; PROTEOMICS;

EID: 77951207114     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2010.03.002     Document Type: Article

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