1 mm ID poly(styrene-co-divinylbenzene) monolithic columns for high-peak capacity one- and two-dimensional liquid chromatographic separations of intact proteins

Journal of Separation Science

Volumn 32, Issue 15-16, 2009, Pages 2504-2509

  Eeltink, Sebastiaan ^a   Dolman, Sebastiaan ^a   Detobel, Frederik ^b   Desmet, Gert ^b   Swart, Remco ^a   Ursem, Mario ^a  

^a Dionex Corporation   (Netherlands)

^b VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

Author keywords

Automated offline 2D LC; Loadability; Peak capacity

Indexed keywords

ANALYSIS TIME; AUTOSAMPLERS; COLUMN TEMPERATURE; FRACTION COLLECTION; GRADIENT TIME; INTACT PROTEINS; LIQUID CHROMATOGRAPHIC SEPARATIONS; LOADABILITY; MIN PROTEINS; MONOLITHIC COLUMNS; OFF-LINE 2D-LC; OFFLINE; OPTIMIZED CONDITIONS; PEAK CAPACITY; PEAK HEIGHT; PEAK WIDTHS; POLY(STYRENE-CO-DIVINYLBENZENE);

ESCHERICHIA COLI; FRACTIONATION; ION EXCHANGE; LIQUID CHROMATOGRAPHY; LIQUID CRYSTAL POLYMERS; STYRENE; TWO DIMENSIONAL;

PROTEINS;

CYTOCHROME C; POLY(STYRENE CO DIVINYLBENZENE); POLYMER; UNCLASSIFIED DRUG; CARBONATE DEHYDRATASE; DIVINYLBENZENE POLYSTYRENE COPPOLYMER; DIVINYLBENZENE-POLYSTYRENE COPPOLYMER; ESCHERICHIA COLI PROTEIN; MYOGLOBIN; PANCREATIC RIBONUCLEASE; POLYSTYRENE DERIVATIVE;

ACCURACY; ANION EXCHANGE; ARTICLE; CHEMICAL STRUCTURE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; DENSITY GRADIENT; ESCHERICHIA COLI; FLOW RATE; LIQUID CHROMATOGRAPHY; MONOLITHIC CHROMATOGRAPHY; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; REVERSED PHASE LIQUID CHROMATOGRAPHY; SEPARATION TECHNIQUE; TEMPERATURE; CHEMISTRY; EVALUATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INSTRUMENTATION; ISOLATION AND PURIFICATION; METHODOLOGY;

ESCHERICHIA COLI;

CARBONIC ANHYDRASES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ESCHERICHIA COLI PROTEINS; MYOGLOBIN; POLYSTYRENES; RIBONUCLEASE, PANCREATIC;

EID: 70350502142     PISSN: 16159306     EISSN: 16159314     Source Type: Journal    
DOI: 10.1002/jssc.200900068     Document Type: Article

References (27)

1. Kubin, M., Spacek, P., Chromecek, R., Collect. Czech. Chem. Commun. 1967, 32, 3881-3887.
2. Hansen, L. C., Sievers, R. E., J. Chromatogr. A 1974, 99, 123-133.
3. Xie, S., Svec, F., Fréchet, J. M. J., J. Chromatogr. A 1997, 775, 65-72.
4. Svec, F., J. Sep. Sci. 2004, 27, 747-766.
5. Josic, D., Clifton, J. G., J. Chromatogr. A 2007, 1144, 2-13.
6. Levkin, P. A., Eeltink, S., Stratton, T. R., Brennen, R., Robotti, K., Yin, H., Killeen, K., Svec, F., Fréchet, J. M. J., J. Chromatogr. A 2008, 1200, 55-61.
7. Koide, T., Uneno, K., J. Chromatogr. A 2001, 909, 305-315.
8. Courtois, J., Szumski, M., Bystrom, E., Iwasiewicz, A., Shchukarev, A., Irgum, K., J. Sep. Sci. 2006, 29, 14-24.
9. Courtois, J., Szumski, M., Georgsson, F., Irgum, K., Anal. Chem. 2007, 79, 335-344.
10. Chambers, S. T., Glenn, K. M., Lucy, C. A., J. Sep. Sci. 2007, 30, 1628-1645.
11. Mallik, R., Hage, D. S., J. Sep. Sci. 2006, 29, 1682-1704.
12. Svec, F., Electrophoresis 2006, 27, 947-961.
13. Peters, E. C., Petro, M., Svec, F., Fréchet, J. M. J., Anal. Chem. 1998, 70, 2288-2295.
14. Schlemmer, B., Gatschelhofer, C., Pieber, T. R., Sinner, F. M., Buchmeiser, M. R., J. Chromatogr. A 2006, 1132, 124-131.
15. Viklund, C., Svec, F., Fréchet, J. M. J., Irgum, K., Chem. Mater. 1996, 8, 744-750.
16. Arrua, R. D., del Barrio, J. S. R., Ruiz, A. G., Strumia, M., Igarzabal, C. I. A., Mater. Chem. Phys. 2008, 112, 1055-1060.
17. Bisjak, C. P., Trojer, L., Lubbad, S. H., Wieder, W., Bonn, G. K., J. Chromatogr. A 2007, 1154, 229-276.
18. Wieder, W., Lubbad, S. H., Trojer, L., Bisjak, C. P., Bonn, G. K., J. Chromatogr. A 2008, 1191, 253-262.
19. Eeltink, S., Herrero-Martinez, J. M., Rozing, G. P., Schoenmakers, P. J., Kok, W. Th., Anal. Chem. 2005, 77, 7342-7347.
20. Eeltink, S., Svec, F., Electrophoresis 2007, 28, 137-147.
21. Wang, Q. C., Svec, F., Fréchet, J. M. J., Anal. Chem. 1993, 65, 2243-2248.
22. Petro, M., Svec, F., Fréchet, J. M. J., J. Chromatogr. A 1996, 752, 59-66.
23. Huber, C. G., Kleindienst, G., Bonn, G., Chromatographia 1997, 44, 438-448.
24. Premstaller, A., Oberacher, H., Huber, C. G., Anal. Chem. 2000, 72, 4386-4393.
25. Premstaller, A., Oberacher, H., Walcher, W., Huber, C. G., Anal. Chem. 2001, 73, 2390-2396.
26. Ivanov, A. R., Zang, L., Karger, B. L., Anal. Chem. 2003, 75, 5306-5316.
27. Eeltink, S., Dolman, S., Swart, R., Ursem, M., Schoenmakers, P. J., J. Chromatogr. A, in press.