Evaluation of different methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the northeast of Brazil

Brazilian Journal of Microbiology

Volumn 41, Issue 2, 2010, Pages 316-320

  de Sousa Júnior, Francisco Canindé ^a   Néri, Gildelane da Silva ^a   Silva, Ana Karine ^a   de Araújo, Bibiana Priscila Rodrigues Câmara ^a   Guerra, Myrian Júlia de Paiva Dourado ^a   Fernandes, Maria José de Britto Costa ^a   Milan, Eveline Pipolo ^a   de Melo, Maria Celeste Nunes ^a  

^a FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE   (Brazil)

Author keywords

Cefoxitin; Heterogeneous resistance; Methicillin resistance; MRSA; PBP2a

Indexed keywords

METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS AUREUS;

EID: 77950979046     PISSN: 15178382     EISSN: 16784405     Source Type: Journal    
DOI: 10.1590/s1517-83822010000200009     Document Type: Article

References (24)

1. Anand, K.B.; Agrawal, P.; Kumar, S.; Kapila, K. (2009). Comparison of cefoxitin disc diffusion test, oxacillin screen agar, and PCR for mecA gene for detection of MRSA. Indian J. Med. Microbiol. 27 (1), 27-29.
2. Baddour, M.M.; AbuElKheir, M.M.; Fatani, A.J. (2007). Comparison of mecA polymerase chain reaction with phenotypic methods for the detection of methicillin-resistant Staphylococcus aureus. Curr. Microbiol. 55 (6), 473-479.
3. Boutiba-Ben Boubaker, I.; Ben Abbes, R.; Ben Abdallah, H.; Mamlouk, K.; Mahjoubi, F.; Kammoun, A.; Hammami, A.; Ben Redjeb, S. (2004). Evaluation of a cefoxitin disk diffusion test for the routine detection of methicillin-resistant Staphylococcus aureus. Clin. Microbiol. Infect. 10 (8), 762-765.
4. Cauwelier, B.; Gordts, B.; Descheemaecker, P.; Van Landuyt, H. (2004). Evaluation of a disk diffusion method with cefoxitin (30 microg) for detection of methicillin-resistant Staphylococcus aureus. Eur. J. Clin. Microbiol. Infect. Dis. 23 (5), 389-392.
5. CLSI (Clinical and Laboratory Standards Institute). (2007). Performance standards for antimicrobial disk susceptibility tests, 9th edn. Approved Standard M2-A9.Wayne, PA: document M100-S17.
6. Felten, A.; Grandry, B.; Lagrange, P.H.; Casin, I. (2002). Evaluation of three techniques for detection of low-level methicillin-resistant Staphylococcus aureus (MRSA): a disk diffusion method with cefoxitin and moxalactam, the vitek 2 system, and the MRSA-screen latex agglutination test. J. Clin. Microbiol. 40 (8), 2766-2771.
7. Fernandes, C.J.; Fernandes, L.A.; Collignon, P. (2005). Cefoxitin resistance as a surrogate marker for the detection of methicillin-resistant Staphylococcus aureus. J. Antimicrob. Chemother. 55 (4), 506-510.
8. Hamdad, F.; Donda, F.; Lefebvre, J.F.; Laurans, G.; Biendo, M.; Thomas, D.; Canarelli, B.; Rousseau, F.; Eb, F. (2006). Detection of methicillin/oxacillin resistance and typing in aminoglycoside-susceptible methicillin-resistant and kanamycin-tobramycin-resistant methicilin- susceptible Staphylococcus aureus. Microb. Drug Resist. 12 (3), 177-185.
9. Hanssen, A.M.; Ericson Sollid, J.U. (2006). SCCmec in staphylococci: genes on the move. FEMS Immunol Med Microbiol. 46(1), 8-20.
10. Kluytmans, J.; Struelens, M. (2009). Meticillin resistant Staphylococcus aureus in the hospital. BMJ. 12 (1), 338-364.
11. Melo, M.C.N.; Silva-Carvalho, M.C.; Ferreira, R.L.; Coelho, L.R.; Souza, R.R.; Gobbi, C.N.; Rozenbaum, R.; Solari, C.A.; Ferreira-Carvalho, B.T.; Figueiredo, A.M.S. (2004). Detection and molecular characterization of a gentamicin-susceptible, methicillin-resistant Staphylococcus aureus (MRSA) clone in Rio de Janeiro that resembles the NewYork/Japanese clone. J. Hosp. Infec. 58 (4), 276-285.
12. Oliveira, D.C.; de Lencastre, H. (2002). Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 46 (7), 2155-2161.
13. Pacheco, A.B.; Guth, B.E.; Soares, K.C.; Nishimura, L.; Almeida, D.F.; Ferreira, L.C. (1997). Random amplification of polymorphic DNA reveals serotype-specific clonal clusters among enterotoxigenic Escherichia coli strains isolated from humans. J. Clin. Microbiol. 35 (6), 1521-1525.
14. Rezende, C.A.; Figueiredo, A.M.S. (1997). Discrimination of methicillin- resistant Staphylococcus aureus from borderline-resistant and susceptible isolates by different methods. J. Med. Microbiol. 46 (2), 145-149.
15. Ribeiro, A.; Coronado, A.Z.; Silva-Carvalho, M.C.; Ferreira-Carvalho, B.T.; Dias, C.; Rozenbaum, R.; Del Peloso, P.F.; da Costa Ferreira Leite, C.; Teixeira, L.A.; Figueiredo, A.M. (2007). Detection and characterization of international community-acquired infections by methicillin-resistant Staphylococcus aureus clones in Rio de Janeiro and Porto Alegre cities causing both community- and hospital-associated diseases. Diagn. Microbiol. Infect. Dis. 59 (3), 339-345.
16. Sharp, S.E.; Warren, J.A.; Thomson, R.B. Jr. (2005). Cefoxitin disk diffusion screen for confirmation of oxacillin-resistant Staphylococcus aureus isolates and utility in the clinical laboratory. Diagn. Microbiol. Infect. Dis. 51 (1), 69-71.
17. Silva-Carvalho, M.C.; Teixeira, L.A.; Ferreira, F.A.; Ribeiro, A.; Ferreira-Carvalho, B.T.; Figueiredo, A.M. (2009). Comparison of different methods for detecting methicillin resistance in MRSA isolates belonging to international lineages commonly isolated in the American continent. Microbiol. Immunol. 53 (2), 117-122.
18. Skov, R.; Smyth, R.; Clausen, M.; Larsen, A.R.; Frimodt-Muller, N.; Olsson-Liljequist, B.; Kahlmeter, G. (2003). Evaluation of a cefoxitin 30 g disc on Iso-Sensitest agar for detection of methicillin-resistant Staphylococcus aureus. J. Antimicrob. Chemother. 52 (2), 204-207.
19. Skov, R.; Smyth, R.; Larsen, A.R.; Bolmstrôm, A.; Karlsson, A.; Mills, K.; Frimodt-Moller, N.; Kahlmeter, G. (2006). Phenotypic detection of methicillin resistance in Staphylococcus aureus by disk diffusion testing and Etest on Mueller-Hinton agar. J. Clin. Microbiol. 44 (12), 4395-4399.
20. Sousa-Junior, F.C.; Silva-Carvalho, M.C.; Fernandes, M.J.B.C.; Vieira, M.F.P.; Pellegrino, F.L.P.C.; Figueiredo A.M.S.; Melo, M.C.N.; Milan, E.P. (2009). Genotyping of methicillin-resistant Staphylococcus aureus isolates obtained in the Northeast region of Brazil. Braz. J. Med. Biol. Res. 42 (10): 877-881.
21. Swenson, J.M.; Lonsway, D.; McAllister, S.; Thompson, A.; Jevitt, L.; Zhu, W.; Patel, J.B. (2007). Detection of mecA-mediated resistance using reference and commercial testing methods in a collection of Staphylococcus aureus expressing borderline oxacillin MICs. Diagn. Microbiol. Infect. Dis. 58 (1), 33-39.
22. Tomasz, A.; Nachman, S.; Laef, H. (1991). Stable classes of phenotypic expression in methicilin-resistant clinical isolates of staphylococci. Antimicrob. Agents Chemother. 35 (1), 124-129.
23. Velasco, D.; Tomas, M.M.; Cartelle, M.; Beceiro, A.; Perez, A.; Molina, F.; Moure, R.; Villanueva, R.; Bou, G. (2005). Evaluation of different methods for detecting methicillin (oxacillin) resistance in Staphylococcus aureus. J. Antimicrob. Chemother. 55 (3), 379-382.
24. Witte, W.; Pasemann, B.; Cuny, C. (2007). Detection of low-level oxacillin resistance in mecA-positive Staphylococcus aureus. Clin. Microbiol. Infect. 13 (4), 408-412.