Random amplification of polymorphic DNA reveals serotype-specific clonal clusters among enterotoxigenic Escherichia coli strains isolated from humans

Journal of Clinical Microbiology

Volumn 35, Issue 6, 1997, Pages 1521-1525

  Pacheco, Ana B F ^a   Guth, Beatriz E C ^b   Soares, Kellen C C ^a   Nishimura, Lucilia ^b   De Almeida, Darcy F ^a   Ferreira, Luís C S ^a  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; OUTER MEMBRANE PROTEIN; VIRULENCE FACTOR;

ARTICLE; BACTERIUM ISOLATION; BIODIVERSITY; BRAZIL; ENDEMIC DISEASE; ESCHERICHIA COLI; GENETIC ANALYSIS; NONHUMAN; PHENOTYPE; POPULATION GENETICS; PRIORITY JOURNAL; RANDOM AMPLIFIED POLYMORPHIC DNA; SEROTYPE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030915103     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.35.6.1521-1525.1997     Document Type: Article

References (28)

1. Alos, J. I., T. Lambert, and P. Courvalin. 1993. Comparison of two molecular methods for tracing nosocomial transmission of Escherichia coli K1 in a neonatal unit. J. Clin. Microbiol. 31:1704-1709.
2. Armstrong, J., A. Gibbs, R. Peakall, and G. Weiller. 1994. RAPDistance programs version 1.03 for the analysis of patterns of RAPD fragments. Australian National University, Canberra, Australia.
3. Belkum, A. 1994. DNA fingerprinting of medically important microorganisms by use of PCR. Clin. Microbiol. Rev. 7:174-184.
4. Binsztein, N., M. J. Jouve, G. I. Viboud, L. L. Moral, M. Rivas, I. Orskov, C. Ähren, and A. M. Svennerholm. 1991. Colonization factors of enterotoxigenic Escherichia coli isolated from children with diarrhea in Argentina. J. Clin. Microbiol. 29:1893-1898.
5. Brikun, I., K. Suziedelis, and D. E. Berg. 1994. DNA sequence divergence among derivatives of Escherichia coli K-12 detected by arbitrary primer PCR (random amplified polymorphic DNA) fingerprinting. J. Bacteriol. 176:1673-1682.
6. Caugant, D. A., B. R. Levin, I. Orskov, F. Orskov, C. S. Eden, and R. K. Selander. 1985. Genetic diversity in relation to serotype in Escherichia coli. Infect. Immun. 49:407-413.
7. Gomes, T. A. T., V. Rassi, K. L. McDonald, S. R. T. S. Ramos, L. R. Trabulsi, M. A. M. Vieira, B. E. C. Guth, J. A. N. Candeias, C. Ivey, M. R. F. Toledo, and P. A. Blake. 1991. Enteropathogens associated with acute diarrheal disease in urban infants in São Paulo, Brazil. J. Infect. Dis. 164:331-337.
8. Guth, B. E. C., and L. R. Trabulsi. 1985. Evaluation of antisera used for detecting enterotoxigenic Escherichia coli in São Paulo. J. Clin. Microbiol. 22:626-628.
9. Guth, B. E. C., R. M. Silva, M. R. F. Toledo, T. M. O. Lima, and L. R. Trabulsi. 1989. Virulence factors and biochemical characteristics of serotype of Escherichia coli serogroup O29. J. Clin. Microbiol. 27:2161-2164.
10. Guth, B. E. C., E. G. Aguiar, P. M. Griffin, S. R. T. S. Ramos, and T. A. T. Gomes. 1994. Prevalence of colonization factor antigens (CFAs) and adherence to HeLa cells in enterotoxigenic Escherichia coli isolated from feces of children in São Paulo. Microbiol. Immunol. 38:695-699.
11. Jaccard, P. 1901. Étude comparative de la distribution florate dans une portion des Alpes et des Jura. Bull. Soc. Vaudoise Sci. Nat. 37:547-579.
12. Kumar, S., K. Tamura, and M. Nei. 1443. MEGA: molecular evolutionary genetics analysis, version 1.02. The Pennsylvania State University, University Park, Pa.
13. Levine, M. M. 1987. Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, enteroadherent. J. Infect. Dis. 155:377-389.
14. Nishimura, L. S., L. C. S. Ferreira, A. B. F. Pacheco, and B. E. C. Guth. 1996. Relationship between outer membrane protein and lipopolysaccharide profiles and serotypes of enterotoxigenic Escherichia coli isolated in Brazil. FEMS Microbiol. Lett. 143:253-258.
15. Ochman, H., and R. K. Selander. 1984. Evidence for clonal population structure in Escherichia coli. Proc. Natl. Acad. Sci. USA 81:198-201.
16. Orskov, F., I. Orskov, D. J. Evans, R. B. Sack, D. A. Sack, and T. Wadström. 1976. Special Escherichia coli serotypes among enterotoxigenic strains from diarrhoea in adults and children. Med. Microbiol. Immunol. 162:73-80.
17. Orskov, F., and I. Orskov. 1983. Summary of a workshop on the clone concept in the epidemiology, taxonomy, and evolution of the enterobacteriaceae and other bacteria. J. Infect. Dis. 148:346-357.
18. Orskov, F., T. S. Whittam, A. Cravioto, and I. Orskov. 1990. Clonal relationships among classic enteropathogenic Escherichia coli (EPEC) belonging to different O groups. J. Infect. Dis. 162:76-81.
19. Orskov, I., and F. Orskov. 1977. Special O:K:H serotypes among enterotoxigenic E. coli strains from diarrhea in adults and children. Occurrence of the CF (colonization factor) antigen and of hemagglutinating abilities. Med. Microbiol. Immunol. 163:99-110.
20. Pacheco, A. B. F., B. E. C. Guth, D. F. de-Almeida, and L. C. S. Ferreira. 1996. Characterization of enterotoxigenic Escherichia coli by random amplification of polymorphic DNA. Res. Microbiol. 147:175-182.
21. Reis, M. H. L., D. P. Matos, A. F. Pestana-de-Castro, M. R. F. Toledo, and L. R. Trabulsi. 1980. Relationship among enterotoxigenic phenotypes, serotypes and sources of strains in enterotoxigenic Escherichia coli. Infect. Imnum. 28:24-27.
22. Reis, M. H. L., B. E. C. Guth, T. A. T. Gomes, J. Murahovischi, and L. R. Trabulsi. 1482. Frequency of of Escherichia coli strains producing heat-labile toxin and heat-stable toxin or both in children with and without diarrhea in São Paulo. J. Clin. Microbiol. 15:1062-1064.
23. Selander, R. K., T. K. Korhonen, V. Väisänen-Rhen, P. H. Williams, P. E. Pattison, and D. A. Caugant. 1986. Genetic relationships and clonal structure of strains of Escherichia coli causing neonatal septicemia and meningitis. Infect. Immun. 52:213-222.
24. Sommerfelt, H., H. Steinsland, H. M. S. Grewal, G. I. Viboud, N. Bhandari, W. Gaastra, A. M. Svennerholm, and M. K. Bhan. 1996. Colonization factors of enterotoxigenic Escherichia coli isolated from children in north India. J. Infect. Dis. 174:768-776.
25. Wang, G., T. S. Whittam, C. M. Berg, and D. E. Berg. 1993. RAPD (arbitrary primer) PCR is more sensitive than multilocus enzyme electrophoresis for distinguishing related bacterial strains. Nucleic Acids Res. 21:5930-5433.
26. Welsh, J., and M. McClelland. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18:7213-7218.
27. Whittam, T. S., and R. A. Wilson. 1988. Genetic relationships among pathogenic Escherichia coli of serogroup O157. Infect. Immun. 56:2467-2473.
28. Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski, and S. V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531-6535.