Phenotypic detection of methicillin resistance in Staphylococcus aureus by disk diffusion testing and etest on Mueller-Hinton agar

Journal of Clinical Microbiology

Volumn 44, Issue 12, 2006, Pages 4395-4399

  Skov, R ^a   Smyth, R ^b   Larsen, A R ^a   Bolmstrom A ^c   Karlsson, A ^c   Mills, K ^c   Frimodt Moller, N ^a   Kahlmeter, G ^b  

^a STATENS SERUM INSTITUT   (Denmark)

^b CENTRAL HOSPITAL   (Sweden)

^c AB BIODISK   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

AGAR; CEFOXITIN; METICILLIN; MUELLER HINTON AGAR; OXACILLIN;

ACCURACY; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATE; CONTROLLED STUDY; DISK DIFFUSION; HUMAN; INCUBATION TIME; MECA GENE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PENICILLIN RESISTANCE; PHENOTYPE; PRIORITY JOURNAL; TEMPERATURE;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; CEFOXITIN; CULTURE MEDIA; FALSE NEGATIVE REACTIONS; METHICILLIN RESISTANCE; MICROBIAL SENSITIVITY TESTS; QUALITY CONTROL; REPRODUCIBILITY OF RESULTS; STAPHYLOCOCCUS AUREUS; TEMPERATURE; TIME FACTORS;

METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS AUREUS;

EID: 33845591534     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.01411-06     Document Type: Article

References (24)

1. Berger-Bachi, B., A. Strassle, J. E. Gustafson, and F. H. Kayser. 1992. Mapping and characterization of multiple chromosomal factors involved in methicillin resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 36:1367-1373.
2. Boutiba-Ben Boubaker, I., R. Ben Abbes, H. Ben Abdallah, K. Mamlouk, F. Mahjoubi, A. Kammoun, A. Hammami, and S. Ben Redjeb. 2004. Evaluation of a cefoxitin disk diffusion test for the routine detection of methicillin-resistant Staphylococcus aureus. Clin. Microbiol. Infect. 10:762-765.
3. Boyce, J. M., B. Cookson, K. Christiansen, S. Hori, J. Vuopio-Varkila, S. Kocagoz, A. Y. Oztop, C. M. Vandenbroucke-Grauls, S. Harbarth, and D. Pittet. 2005. Methicillin-resistant Staphylococcus aureus. Lancet Infect. Dis. 5:653-663.
4. Cauwelier, B., B. Gordts, P. Descheemaecker, and H. Van Landuyt. 2004. Evaluation of a disk diffusion method with cefoxitin (30 μg) for detection of methicillin-resistant Staphylococcus aureus. Eur. J. Clin. Microbiol. Infect. Dis. 23:389-392.
5. Chambers, H. F. 1997. Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin. Microbiol. Rev. 10:781-791.
6. Clinical and Laboratory Standards Institute. 2005. Performance standards for antimicrobial susceptibility testing. 15th informational supplement M100-S15. Clinical and Laboratory Standards Institute, Wayne, PA.
7. Enright, M. C., N. P. Day, C. E. Davies, S. J. Peacock, and B. G. Spratt. 2000. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J. Clin. Microbiol. 38:1008-1015.
8. Felten, A., B. Grandry, P. H. Lagrange, and I. Casin. 2002. Evaluation of three techniques for detection of low-level methicillin-resistant Staphylococcus aureus (MRSA): a disk diffusion method with cefoxitin and moxalactam, the Vitek 2 system, and the MRSA-screen latex agglutination test. J. Clin. Microbiol. 40:2766-2771.
9. Fernandes, C. J., L. A. Fernandes, and P. Collignon. 2005. Cefoxitin resistance as a surrogate marker for the detection of methicillin-resistant Staphylococcus aureus. J. Antimicrob. Chemother. 55:506-510.
10. Gueudet, T., and C. Lemble. 2004. Comparison of five usual techniques for detection of methicillin-resistant Staphylococcus aureus. Pathol. Biol. (Paris) 52:617-621.
11. Harmsen, D., H. Claus, W. Witte, J. Rothganger, H. Claus, D. Turnwald, and U. Vogel. 2003. Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J. Clin. Microbiol. 41:5442-5448.
12. Heneine, N., and P. R. Stewart. 1986. Physiological determination of methicillin resistance in Staphylococcus aureus: comparison of clinical and genetically derived isolates. J. Antimicrob. Chemother. 17:705-715.
13. McDougal, L. K., C. D. Steward, G. E. Killgore, J. M. Chaitram, S. K. McAllister, and F. C. Tenover. 2003. Pulsed-field gel electrophoresis typing of oxacillin-resistant Staphylococcus aureus isolates from the United States: establishing a national database. J. Clin. Microbiol. 41:5113-5120.
14. Mougeot, C., J. Guillaumat-Tailliet, and J. M. Libert. 2001. Staphylococcus aureus: new detection of intrinsic resistance using the diffusion method. Pathol. Biol. (Paris) 49:199-204.
15. Murchan, S., M. E. Kaufmann, A. Deplano, R. de Ryck, M. Struelens, C. E. Zinn, V. Fussing, S. Salmenlinna, J. Vuopio-Varkila, N. El Solh, C. Cuny, W. Witte, P. T. Tassios, N. Legakis, W. van Leeuwen, A. van Belkum, A. Vindel, I. Laconcha, J. Garaizar, S. Haeggman, B. Olsson-Liljequist, U. Ransjo, G. Coombes, and B. Cookson. 2003. Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J. Clin. Microbiol. 41:1574-1585.
16. Pottumarthy, S., T. R. Fritsche, and R. N. Jones. 2005. Evaluation of alternative disk diffusion methods for detecting mecA-mediated oxacillin resistance in an international collection of staphylococci: validation report from the SENTRY Antimicrobial Surveillance Program. Diagn. Microbiol. Infect. Dis. 51:57-62.
17. Sabath, L. D., and S. J. Wallace. 1971. The problems of drug-resistant pathogenic bacteria. Factors influencing methicillin resistance in staphylococci. Ann. N. Y. Acad. Sci. 182:258-266.
18. Skov, R., L. V. Pallesen, R. L. Poulsen, and F. Espersen. 1999. Evaluation of a new three hours hybridisation method for detection of the mecA gene in Staphylococcus aureus and correlation to existing genotypic and phenotypic susceptibility testing methods. J. Antimicrob. Chemother. 43:467-475.
19. Skov, R., R. Smyth, M. Clausen, A. R. Larsen, N. Frimodt-Moller, B. Olsson-Liljequist, and G. Kahlmeter. 2003. Evaluation of a cefoxitin 30 μg disc on Iso-Sensitest agar for detection of methicillin-resistant Staphylococcus aureus. J. Antimicrob. Chemother. 52:204-207.
20. Skov, R., R. Smyth, A. R. Larsen, N. Frimodt-Moller, and G. Kahlmeter. 2005. Evaluation of cefoxitin 5 and 10 μg discs for the detection of methicillin resistance in staphylococci. J. Antimicrob. Chemother. 55:157-161.
21. Swenson, J. M., and F. C. Tenover. 2005. Results of disk diffusion testing with cefoxitin correlate with presence of mecA in Staphylococcus spp. J. Clin. Microbiol. 43:3818-3823.
22. Technical Committee ISO/TC 212 and Technical Committee CEN/TC 140. 2006. Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices - part 1: reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. International Organization for Standardization, Geneva, Switzerland.
23. Urbaskova, P., O. Melter, B. Mackova, V. Jakubu, and M. Wunschova. 2004. Detection of MRSA in a group of 752 strains of S. aureus using a cefoxitin disk. Epidemiol. Mikrobiol. Imunol. 53:62-65.
24. Velasco, D., T. M. del Mar, M. Cartelle, A. Beceiro, A. Perez, F. Molina, R. Moure, R. Villanueva, and G. Bou. 2005. Evaluation of different methods for detecting methicillin (oxacillin) resistance in Staphylococcus aureus. J. Antimicrob. Chemother. 55:379-382.