Detection of mecA-mediated resistance using reference and commercial testing methods in a collection of Staphylococcus aureus expressing borderline oxacillin MICs

Diagnostic Microbiology and Infectious Disease

Volumn 58, Issue 1, 2007, Pages 33-39

  Swenson, Jana M ^a   Lonsway, David ^a   McAllister, Sigrid ^a   Thompson, Angela ^a   Jevitt, Laura ^a   Zhu, Wenming ^a   Patel, Jean B ^a  

^a NATIONAL CENTER FOR EMERGING AND ZOONOTIC INFECTIOUS DISEASES   (United States)

Author keywords

Cefoxitin; mecA; Oxacillin; Staphylococcus aureus

Indexed keywords

AGAR; CEFOXITIN; OXACILLIN; PENICILLIN BINDING PROTEIN 2A;

ANTIBIOTIC SENSITIVITY; ARTICLE; DIFFUSION; LABORATORY TEST; METHODOLOGY; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCREENING; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS AUREUS;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; CEFOXITIN; DRUG RESISTANCE, BACTERIAL; HUMANS; MICROBIAL SENSITIVITY TESTS; OXACILLIN; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; REFERENCE STANDARDS; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS AUREUS;

EID: 34247877611     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2006.10.022     Document Type: Article

References (42)

1. Andrews J., Brenwald N., Brown D.F.J., Perry J., King A., Gemmell C., and for the B.W.P.o.S.T. Evaluation of a 10 μg cefoxitin disc for the detection of methicillin resistance in Staphylococcus aureus by BSAC methodology. J. Antimicrob. Chemother. 56 (2005) 599-600
2. Barg N., Chambers H., and Kernodle D. Borderline susceptibility to antistaphylococcal penicillins is not conferred exclusively by the hyperproduction of beta-lactamase. Antimicrob. Agents Chemother. 35 (1991) 1975-1979
3. Bignardi G.E., Woodford N., Chapman A., Johnson A.P., and Speller D.C.E. Detection of the mec-A gene and phenotypic detection of resistance in Staphylococcus aureus isolates with borderline or low-level methicillin resistance. J. Antimicrob. Chemother. 37 (1996) 53-63
4. Boutiba-Ben Boubaker I., Ben Abbes R., Ben Abdallah H., Mamlouk K., Mahjoubi F., Kammoun A., Hammami A., and Ben Redjeb S. Evaluation of a cefoxitin disk diffusion test for the routine detection of methicillin-resistant Staphylococcus aureus. Clin. Microbiol. Infect. 10 (2004) 762-765
5. Bressler A.M., Williams T., Culler E.E., Zhu W., Lonsway D., Patel J.B., and Nolte F.S. Correlation of penicillin binding protein 2a detection with oxacillin resistance in Staphylococcus aureus and discovery of a novel penicillin binding protein 2a mutation. J. Clin. Microbiol. 43 (2005) 4541-4544
6. Cauwelier B., Gordts B., Descheemaecker P., and Van Landuyt H. Evaluation of a disk diffusion method with cefoxitin (30 mg) for detection of methicillin-resistant Staphylococcus aureus. Eur. J. Clin. Microbiol. Infect. Dis. 23 (2004) 389-392
7. Chambers H.F., Hartman B.J., and Tomasz A. Increased amounts of a novel penicillin-binding protein in a strain of methicillin-resistant Staphylococcus aureus exposed to nafcillin. J. Clin. Invest. 76 (1985) 325-331
8. Chambers H.F., Archer G., and Matsuhashi M. Low-level methicillin resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 33 (1989) 424-428
9. Chambers H.F. Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin. Microbiol. Rev. 10 (1997) 781-791
10. Clinical and Laboratory Standards Institute/NCCLS. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard-Sixth Edition. NCCLS Document M7-A6 (2003), Clinical and Laboratory Standards Institute, Wayne, PA
11. Clinical and Laboratory Standards Institute/NCCLS. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard-Eighth Edition. NCCLS Document M2-A8 (2003), Clinical and Laboratory Standards Institute, Wayne, PA
12. Fang H., and Hedin G. Use of cefoxitin-based selective broth for improved detection of methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 44 (2006) 592-594
13. Felten A., Grandry B., Lagrange P.H., and Casin I. Evaluation of three techniques for detection of low-level methicillin-resistant Staphylococcus aureus (MRSA): a disk diffusion method with cefoxitin and moxalactam, the Vitek 2 system, and the MRSA-Screen latex agglutination test. J. Clin. Microbiol. 40 (2002) 2766-2771
14. Fernandes C.J., Fernandes L.A., Collignon P., and on behalf of the Australian Group on Antimicrobial Resistance. Cefoxitin resistance as a surrogate marker for the detection of methicillin-resistant Staphylococcus aureus. J. Antimicrob. Chemother. 55 (2005) 506-510
15. Flayhart D., Hindler J.F., Bruckner D.A., Hall G., Shrestha R.K., Vogel S.A., Richter S.S., Howard W., Walther R., and Carroll K.C. Multicenter evaluation of BBL CHROMagar MRSA medium for direct detection of methicillin-resistant Staphylococcus aureus from surveillance cultures of the anterior nares. J. Clin. Microbiol. 43 (2005) 5536-5540
16. Hackbarth C.J., Kocagoz T., Kocagoz S., and Chambers H.F. Point mutations in Staphylococcus aureus PBP 2 gene affect penicillin-binding kinetics and are associated with resistance. Antimicrob. Agents Chemother. 39 (1995) 103-106
17. Hedin G., and Fang H. Evaluation of Two New Chromogenic Media, CHROMagar MRSA and S. aureus ID, for Identifying Staphylococcus aureus and Screening Methicillin-Resistant S. aureus. J. Clin. Microbiol. 43 (2005) 4242-4244
18. Hirano L., and Bayer A.S. Beta-Lactam-beta-lactamase-inhibitor combinations are active in experimental endocarditis caused by beta-lactamase-producing oxacillin-resistant staphylococci. Antimicrob. Agents Chemother. 35 (1991) 685-690
19. Killgore G.E., Holloway B., and Tenover F.C. A 5′ nuclease PCR (TaqMan) high-throughput assay for detection of the mecA gene in staphylococci. J. Clin. Microbiol. 38 (2000) 2516-2519
20. Kline M.W., Mason Jr. E.O., and Kaplan S.L. Outcome of heteroresistant Staphylococcus aureus infections in children. J. Infect. Dis. 156 (1987) 205-208
21. Massidda O., Montanari M.P., and Varaldo P.E. Evidence for a methicillin-hydrolyzing β-lactamase in Staphylococcus aureus strains with borderline susceptibility to this drug. FEMS Microbiol. Lett. 92 (1992) 223-228
22. McDougal L.K., and Thornsberry C. The role of beta-lactamase in staphylococcal resistance to penicillinase-resistant penicillins and cephalosporins. J. Clin. Microbiol. 23 (1986) 832-839
23. Mougeot C., Guillaumat-Taillet J., and Libert J.M. Staphylococcus aureus: nouvelle detection de la resistance intrinseque par la methode de diffusion. Pathol. Biol. 49 (2001) 199-204
24. Perry J.D., Davies A., Butterworth L.A., Hopley A.L.J., Nicholson A., and Gould F.K. Development and evaluation of a chromogenic agar medium for methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 42 (2004) 4519-4523
25. Pottumarthy S., Fritsche T.R., and Jones R.N. Evaluation of alternative disk diffusion methods for detecting mecA-mediated oxacillin resistance in an international collection of staphylococci: validation report from the SENTRY antimicrobial surveillance program. Diagn. Microbiol. Infect. Dis. 51 (2005) 57-62
26. Sakoulas G., Gold H.S., Venkataraman L., DeGirolami P., Eliopoulos G.M., and Qian Q. Methicillin-resistant Staphylococcus aureus: comparison of susceptibility testing methods and analysis of mecA-positive susceptible strains. J. Clin. Microbiol. 39 (2001) 3946-3951
27. Sharp S.E., Warren J.A., and Thomson Jr. R.B. Cefoxitin disk diffusion screen for confirmation of oxacillin-resistant Staphylococcus aureus isolates and utility in the clinical laboratory. Diagn. Microbiol. Infect. Dis. 51 (2005) 69-71
28. Sierra-Madero J.G., Knapp C., Karaffa C., and Washington J.A. Role of beta-lactamase and different testing conditions in oxacillin-borderline-susceptible staphylococci. Antimicrob. Agents Chemother. 32 (1988) 1754-1757
29. Skov R., Smyth R., Clausen M., Larsen A.R., Frimodt-Moller N., Olsson-Liljequist B., and Kahlmeter G. Evaluation of a cefoxitin 30 mg disc on Iso-Sensitest agar for detection of methicillin-resistant Staphylococcus aureus. J. Antimicrob. Chemother. 52 (2003) 204-207
30. Skov R., Smyth R., Larsen A.R., Frimodt-Moller N., and Kahlmeter G. Evaluation of cefoxitin 5 and 10 μg discs for the detection of methicillin resistance in staphylococci. J. Antimicrob. Chemother. 55 (2005) 157-161
31. Skov R.L., Palleson L.V., Poulsen R.L., and Espersen F. Evaluation of a new 3-h hybridization method for detecting the mecA gene in Staphylococcus aureus and comparison with existing genotypic and phenotypic susceptibility testing methods. J. Antimicrob. Chemother. 43 (1999) 467-475
32. Smyth R.W., and Kahlmeter G. Mannitol salt agar-cefoxitin combination as a screening medium for methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 43 (2005) 3797-3799
33. Spanu T., Sanguinetti M., D'Inezo T., Ciccaglione D., Romano L., Leone F., Mazzella P., and Fadda G. Identification of methicillin-resistant isolates of Staphylococcus aureus and coagulase-negative staphylococci responsible for bloodstream infections with the Phoenix system. Diagn. Microbiol. Infect. Dis. 48 (2004) 221-227
34. Stoakes L., Reyes R., Daniel J., Lennox G., John M.A., Lannigan R., and Hussain Z. Prospective Comparison of a New Chromogenic Medium, MRSASelect, to CHROMagar MRSA and Mannitol-Salt Medium Supplemented with Oxacillin or Cefoxitin for Detection of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 44 (2006) 637-639
35. Swenson J.M., Tenover F.C., and The Cefoxitin Disk Study Group. Results of disk diffusion testing with cefoxitin correlate with presence of mecA in Staphylococcus spp. J. Clin. Microbiol. 43 (2005) 3818-3823
36. Thauvin-Eliopoulos E., Rice L.B., Eliopoulos G.M., and Moellering Jr. R.C. Efficacy of oxacillin and ampicillin-sulbactam combination in experimental endocarditis caused by β-lactamase-hyperproducing Staphylococcus aureus. Antimicrob. Agents Chemother. 34 (1990) 728-732
37. Tomasz A., Drugeon H.B., de Lencastre H.M., Jabes D., McDougal L., and Bille J. New mechanism for methicillin resistance in Staphylococcus aureus: clinical isolates that lack the PBP 2a gene and contain modified penicillin-binding proteins with modified penicillin-binding capacity. Antimicrob. Agents Chemother. 33 (1989) 1869-1874
38. Tonin E., and Tomasz A. β-Lactam-specific resistant mutants of Staphylococcus aureus. Antimicrob. Agents Chemother. 30 (1986) 577-583
39. Urbaskova P., Melter O., Mackova B., Jakubu V., and Wunschova M. Detection of MRSA in a group of 752 strains of S. aureus using a cefoxitin disk. Epidemiol. Mikrobiol. Imunol. 53 (2004) 62-65
40. Velasco D., del Mar Tomas M., Cartelle M., Becceiro A., Perez A., Molina F., Moure R., Villanueva R., and Bou G. Evaluation of different methods for detecting methicillin (oxacillin) resistance in Staphylococcus aureus. J. Antimicrob. Chemother. 55 (2005) 379-382
41. Wu C.Y., Alborn Jr. W.E., Flokowitsch J.E., Hoskins J., Unal S., Blaszczak L.C., Preston D.A., and Skatrud P.L. Site-directed mutagenesis of the mecA gene from a methicillin-resistant strain of Staphylococcus aureus. J. Bacteriol. 176 (1994) 443-449
42. Yamazumi T., Marshall S.A., Wilke W.W., Diekema D.J., Pfaller M.A., and Jones R.N. Comparison of the Vitek gram-positive susceptibility 106 card and the MRSA-Screen latex agglutination test for determining oxacillin resistance in clinical bloodstream isolates of Staphylococcus aureus. J. Clin. Microbiol. 39 (2001) 53-56