Monitoring the genomic stability of in vitro cultured rat bone-marrow-derived mesenchymal stem cells

Chromosome Research

Volumn 17, Issue 8, 2009, Pages 1025-1039

  Foudah, Dana ^a   Redaelli, Serena ^a   Donzelli, Elisabetta ^a   Bentivegna, Angela ^a   Miloso, Mariarosaria ^a   Dalprà, Leda ^a,b   Tredici, Giovanni ^a  

^a UNIVERSITY OF MILANO BICOCCA   (Italy)

^b SAN GERARDO HOSPITAL   (Italy)

Author keywords

chromosomal status; Cytogenetic analysis; genomic stability; in vitro culture conditions; MSCs differentiation capacity; rat mesenchymal stem cells

Indexed keywords

ANEUPLOIDY; ANIMAL; ARTICLE; BONE MARROW CELL; CELL CULTURE; CELL TRANSFORMATION; COMPARATIVE GENOMIC HYBRIDIZATION; CULTURE TECHNIQUE; CYTOLOGY; GENETICS; GENOMIC INSTABILITY; MESENCHYMAL STEM CELL; RAT;

ANEUPLOIDY; ANIMALS; BONE MARROW CELLS; CELL CULTURE TECHNIQUES; CELL TRANSFORMATION, NEOPLASTIC; CELLS, CULTURED; COMPARATIVE GENOMIC HYBRIDIZATION; GENOMIC INSTABILITY; MESENCHYMAL STEM CELLS; RATS;

EID: 77950899350     PISSN: 09673849     EISSN: 15736849     Source Type: Journal    
DOI: 10.1007/s10577-009-9090-6     Document Type: Article

References (38)

1. Abdallah BM, Kassem M (2009) The use of mesenchymal (skeletal) stem cells for treatment of degenerative diseases: current status and future perspectives. J Cell Physiol 218:9-12
2. Adamovic T, Trossö F, Roshani L et al (2005) Oncogene amplification in the proximal part of chromosome 6 in rat endometrial adenocarcinoma as revealed by combined BAC/PAC FISH, chromosome painting, zoo-FISH, and allelotyping. Genes Chromosomes Cancer 44:139-153 (Pubitemid 41306642)
3. Aguilar S, Nye E, Chan J et al (2007) Murine but not human mesenchymal stem cells generate osteosarcoma-like lesions in the lung. Stem Cells 25:1586-1594 (Pubitemid 47106029)
4. Bernardo ME, Zaffaroni N, Novara F et al (2007) Human bone marrow-derived mesenchymal stem cells do not undergo transformation after long-term in vitro culture and do not exhibit telomere maintenance mechanisms. Cancer Res 67:9142-9149 (Pubitemid 47535900)
5. Brodeur GM, Seeger RC, Schwab M, Varmus HE, Bishop JM (1984) Amplification of N-myc in untreated human neuroblastomas correlates with advanced disease stage. Science 224:1121-1124 (Pubitemid 14106392)
6. Brooke G, Cook M, Blair C et al (2007) Therapeutic applications of mesenchymal stromal cells. Semin Cell Dev Biol 18:846-858 (Pubitemid 350138458)
7. Caplan AI (2007) Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. J Cell Physiol 213:341-347 (Pubitemid 47509711)
8. Committee for a Standardized Karyotype of Rattus Norvegicus (1973) Standard karyotype of the Norway rat, Rattus norvegicus. Cytogenet Cell Genet 12:199-205
9. Doerr HW, Cinatl J, Stürmer M, Rabenau HF (2003) Prions and orthopedic surgery. Infection 31:163-171 (Pubitemid 36737038)
10. Dominici M, Le Blanc K, Mueller I et al (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8:315-317 (Pubitemid 44269669)
11. Donzelli E, Salvadè A, Mimo P et al (2007) Mesenchymal stem cells cultured on a collagen scaffold: in vitro osteogenic differentiation. Arch Oral Biol 52:64-73 (Pubitemid 44853923)
12. Furlani D, Li W, Pittermann E et al (2009) A transformed cell population derived from cultured mesenchymal stem cells has no functional effect after transplantation into the injured heart. Cell Transplant 18:319-331
13. Goffredo D, Conti L, Di Febo F et al (2008) Setting the conditions for efficient, robust and reproducible generation of functionally active neurons from adult subventricular zone-derived neural stem cell. Cell Death Diff 15 (12):1847-1856
14. Guryev V, Saar K, Adamovic T et al (2008) Distribution and functional impact of DNA copy number variation in the rat. Nat Genet 40:538-545 (Pubitemid 351601223)
15. Izadpanah R, Kaushal D, Kriedt C et al (2008) Long-term in vitro expansion alters the biology of adult mesenchymal stem cells. Cancer Res 68:4229-4238
16. Lee KD, Kuo TK, Whang-Peng J et al (2004) In vitro hepatic differentiation of human mesenchymal stem cells. Hepatology 40:1275-1284 (Pubitemid 39657001)
17. Liebman J, Goldberg RL (2001) Chondrocyte culture and assay. Curr Protoc Pharmacol 12:12.2.1-12.2.18
18. Mannello F, Tonti GA (2007) Concise review: no breakthroughs for human mesenchymal and embryonic stem cell culture: conditioned medium, feeder layer, or feeder-free; medium with fetal calf serum, human serum, or enriched plasma; serum-free, serum replacement nonconditioned medium, or ad hoc formula? All that glitters is not gold! Stem Cells 25:1603-1609 (Pubitemid 47121836)
19. Meuleman N, Tondreau T, Delforge A et al (2006) Humanmarrow mesenchymal stem cell culture: serum-free medium allows better expansion than classical alpha-MEM medium. Eur J Haematol 76:309-316 (Pubitemid 43362939)
20. Meza-Zepeda LA, Noer A, Dahl JA, Micci F, Myklebost O, Collas P (2008) High-resolution analysis of genetic stability of human adipose tissue stem cells cultured to senescence. J Cell Mol Med 12:553-563 (Pubitemid 351555134)
21. Miura M, Miura Y, Padilla-Nash HM et al (2006) Accumulated chromosomal instability in murine bone marrow mesenchymal stem cells leads to malignant transformation. Stem Cells 24:1095-1103 (Pubitemid 44464742)
22. Neuhuber B, Swanger SA, Howard L, Mackay A, Fischer I (2008) Effects of plating density and culture time on bone marrow stromal cell characteristics. Exp Hematol 36:1176-1185
23. Pal R, Hanwate M, Jan M, Totey S (2009) Phenotypic and functional comparison of optimum culture conditions for upscaling of bone marrow-derived mesenchymal stem cells. J Tissue Eng Regen Med 3:163-174
24. Røsland GV, Svendsen A, Torsvik A et al (2009) Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation. Cancer Res 69:5331-5339
25. Rubie H, Hartmann O, Michon J et al (1997) N-Myc gene amplification is a major prognostic factor in localized neuroblastoma: results of the French NBL 90 study. Neuroblastoma Study Group of the Société Francaise d'Oncologie Pédiatrique. J Clin Oncol 15:1171-1182 (Pubitemid 27106305)
26. Rubio D, Garcia-Castro J, Martín MC et al (2005) Spontaneous human adult stem cell transformation. Cancer Res 65:3035-3039 (Pubitemid 40524580)
27. Shahdadfar A, Frønsdal K, Haug T, Reinholt FP, Brinchmann JE (2005) In vitro expansion of human mesenchymal stem cells: choice of serum is a determinant of cell proliferation differentiation, gene expression, and transcriptome stability. Stem Cells 23:1357-1366 (Pubitemid 41504923)
28. Shakhova O, Leung C, van Montfort E, Berns A, Marino S (2006) Lack of Rb and p53 delays cerebellar development and predisposes to large cell anaplastic medulloblastoma through amplification of N-Myc and Ptch2. Cancer Res 66:5190-5200 (Pubitemid 43844942)
29. Sotiropoulou PA, Perez SA, Salagianni M, Baxevanis CN, Papamichail M (2006) Cell culture medium composition and translational adult bone marrow-derived stem cell research. Stem Cells 24:1409-1410 (Pubitemid 44464710)
30. Spiliotopoulos D, Goffredo D, Conti L et al (2009) An optimized experimental strategy for efficient conversion of embryonic stem (ES)-derived mouse neural stem (NS) cells into a nearly homogeneous mature neuronal population. Neurobiol Dis 34 (2):320-331
31. Tolar J, Nauta AJ, Osborn MJ et al (2007) Sarcoma derived from cultured mesenchymal stem cells. Stem Cells 25:371-379 (Pubitemid 46232791)
32. Tondreau T, Dejeneffe M, Meuleman N et al (2008) Gene expression pattern of functional neuronal cells derived from human bone marrow mesenchymal stromal cells. BMC Genomics 9:166
33. Tonti GA, Mannello F (2008) From bone marrow to therapeutic applications: different behaviour and genetic/epigenetic stability during mesenchymal stem cell expansion in autologous and foetal bovine sera? Int J Dev Biol 52:1023-1032
34. Wang Y, Huso DL, Harrington J et al (2005) Outgrowth of a transformed cell population derived from normal human BM mesenchymal stem cell culture. Cytotherapy 7:509-519 (Pubitemid 43078081)
35. Wexler SA, Donaldson C, Denning-Kendall P, Rice C, Bradley B, Hows JM (2003) Adult bone marrow is a rich source of human mesenchymal 'stem' cells but umbilical cord and mobilized adult blood are not. Br J Haematol 121:368-374 (Pubitemid 36560606)
36. Yu D, Silva GA (2008) Stem cell sources and therapeutic approaches for central nervous system and neural retinal disorders. Neurosurg Focus 24(3-4):E11
37. Zhang ZX, Guan LX, Zhang K et al (2007) Cytogenetic analysis of human bone marrow-derived mesenchymal stem cells passaged in vitro. Cell Biol Int 31:645-648 (Pubitemid 46660640)
38. Zhou YF, Bosch-Marce M, Okuyama H et al (2006) Spontaneous transformation of cultured mouse bone marrow-derived stromal cells. Cancer Res 66:10849-10854 (Pubitemid 44876981)