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Mosquito larvicidal activity: In brief, concentrations of test compounds were prepared by serial dilution of a stock solution of the compounds in DMSO. Batches of 20-early fourth instar mosquito larvae were placed in 245 mL of degassed distilled water, followed by the addition of 5 mL of DMSO solution containing the test compound in a 500 mL beaker cup, shaken lightly to ensure a homogeneous test solution, and incubated at the room temperature. All the experiments were replicated three times. The control was prepared with 245 mL of degassed distilled water and 5 mL of DMSO solution to which larvae were added. Mortality was recorded after 24 h of exposure, during which no food was given to the larvae and percentage of mortality was corrected if necessary using the formula as per the reference.
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